Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Preparation and Biocompatibility of 6-amino-6-deoxychitosan for Immobilization of Epidermal Growth Factor

세포성장인자 고정화를 위한 6-amino-6-deoxychitosan의 제조와 생체적합성

  • Son, Tae Il (Department of Biotechnology, University of Chung-Ang) ;
  • Park, Se Hoon (Department of Biotechnology, University of Chung-Ang) ;
  • Kang, Hahk Soo (Department of Biotechnology, University of Chung-Ang) ;
  • Jang, Eui Chan (Department of Orthopaedic Surgery, College of Medicine, University of Chung-Ang)
  • 손태일 (중앙대학교 생명공학과) ;
  • 박세훈 (중앙대학교 생명공학과) ;
  • 강학수 (중앙대학교 생명공학과) ;
  • 장의찬 (중앙대학교 의과대학 정형외과)
  • Received : 2004.09.20
  • Accepted : 2005.02.21
  • Published : 2005.04.10
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Abstract

Chitosan derivatives, 6-amino-6-deoxychitosan (6A6DC) was successively prepared as a reactive carbohydrate for the stabilization of epidermal growth factor (EGF) by the reactions of chitosan with tosyl chloride, sodium azide, and lithium aluminum tetrahydride. The structure of 6A6DC was confirmed by EA, FT-IR, $^1H-NMR$ and $^{13}C\{^1H\}-NMR$. The degree of substitution (ds) of amino groups in 6A6DC was determined to be 0.7. 6A6DC did not show any cytotoxicity on the normal human dermal fibroblast (NHDF) proliferation at least in the range tested (0.3 g/mL 600 g/mL) and was considered as a suitable material for the stabilization of EGF against proteolytic degradation due to its non-cytotoxicity and high reactivity.

Chitosan유도체인 6-amino-6-deoxychitosan (6A6DC)은 상피세포 성장인자(EGF)를 안정화시키기 위한 하나의 당으로써, tosyl chloride, sodium azide 그리고 lithium aluminum tetrahydride와의 반응으로부터 성공적으로 제조되었다. 이것의 구조는 원소분석, FT-IR, $^1H$ NMR 및 $^{13}C\{^1H\}$ NMR에 의해 확인되었다. 6A6DC는 amino기의 치환율이 0.7로 나타났으며, $0.3{\mu}g/mL{\sim}600{\mu}g/mL$의 농도범위에서 normal human dermal fibroblast (NHDF)가 증식하는데 어떠한 세포독성도 나타내지 않았다. 따라서, 6A6DC는 자체의 세포무독성과 높은 반응성으로 인하여 단백질 분해효소로부터 EGF를 안정화시키는데 적합한 재료라고 사료된다.

Keywords

Acknowledgement

Supported by : 중앙대학교

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