Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Apoptotic Effects and Mechanism Study of Silymarin in DU145 Cells

전립선 암세포에서 silymarin의 고사 유도 효과 및 기전 연구

  • Kwon Kang-Beom (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim Eun-Kyung (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Park Sung-Joo (Department of herbology, School of Oriental Medicine, Wonkwang University) ;
  • Song Ho-Joon (Department of herbology, School of Oriental Medicine, Wonkwang University) ;
  • Lee Young-Rae (Department of Biochemistry, Institute for Healthcare Technology Development, Medical School, Chonbuk National University) ;
  • Park Byung-Hyun (Department of Biochemistry, Institute for Healthcare Technology Development, Medical School, Chonbuk National University) ;
  • Park Jin-Wzoo (Department of Biochemistry, Institute for Healthcare Technology Development, Medical School, Chonbuk National University) ;
  • Ryu Do-Gon (Department of Physiology, School of Oriental Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실) ;
  • 송호준 (원광대학교 한의과대학 본초학교실) ;
  • 이영래 (전북대학교 의과대학 생화학교실, 전북대학교 헬스케어기술개발사업단) ;
  • 박병현 (전북대학교 의과대학 생화학교실, 전북대학교 헬스케어기술개발사업단) ;
  • 박진우 (전북대학교 의과대학 생화학교실, 전북대학교 헬스케어기술개발사업단) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2005.12.01
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Abstract

Silymarin has been known to exert anti-tumoral activity in Korea. However, its molecular mechanism of action is not understood. In this study, we found that silymarin induced apoptosis in androgen-independent prostate cancer DU145 cells as confirmed by DNA fragmentation. Our data demonstrated that silymarin-induced apoptotic cell death was accompanied by activation of caspase-3 and subsequent cleavages of its substrates, poly(ADP-ribose) polymerase (PARP) in a time- and concentration-dependent manner. Also, silymarin-induced apoptotic mechanism of DU145 cells involved the induction of Par-4 protein expression. Taken together, these results suggest that silymarin induces the activation of caspase-3, degradation of PARP, increase of Par-4 expression, and eventually leads to apoptotic cell death.

Keywords

References

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