In vitro Growth Inhibition and Apoptotic Effects of Hang-baek-Tang on HL-60 Cells

  • Park Jun-Ho (Division of Biological Science, Wonkwang University) ;
  • Ju Sung-Min (Department of Pathology, College of Oriental Medicine, Wokwang University) ;
  • Kim Kun-Jung (Department of Pathology, College of Oriental Medicine, Wokwang University) ;
  • Jeon Byung-Hoon (Department of Pathology, College of Oriental Medicine, Wokwang University) ;
  • Oh Jung-Mi (Division of Bio-Nano Chemistry Wonkwang University) ;
  • Lee Chae-Ho (Division of Bio-Nano Chemistry Wonkwang University) ;
  • Han Dong-Min (Division of Biological Science, Wonkwang University) ;
  • Kim Won-Sin (Division of Biological Science, Wonkwang University)
  • Published : 2005.12.01

Abstract

To develop novel anti-leukemic medicine, we have prepared a Korean traditional medicine, named Hang-baek-Tang, which is composed of 8 kinds of anti-leukemic medicinal plants. The water extracts was examined anti-leukemic activity using the human leukemia cell line, HL-60 cells. HL-60 cells showed the growth inhibition and several apoptotic features, including DNA ladders, morphological changes, by treatment of the cells with Hang-Daek-Tang. We have observed that Hang-baek-Tang induced the activation of caspase-3, caspase-8 and caspase-9. Further molecular analysis demonstrated that Hang-baek-Tang induced cleavage of PARP and increase of hypodiploid (Sub-G1) population in flow cytometric analysis. These results indicate that Hang-baek-Tang has been considered to exert anti-leukemic activity through the procaspase-3 activation pathway.

Keywords

References

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