Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Studies on the Comparison of Antibacterial Activity by Catechin Concentration in Green Tea Extract

녹차 추출물 중 카테킨 성분 농도에 따른 항균활성 비교 연구

  • Choi Kyung-Min (Department of Infection Biology, College of Medicine, Wonkwang University) ;
  • Yun Young-Gab (Department of Oriental Medicine, Graduate School of Wonkwang University) ;
  • Jiang Jing-Hua (Department of Infection Biology, College of Medicine, Wonkwang University) ;
  • Oh Sung-Su (Department of Infection Biology, College of Medicine, Wonkwang University) ;
  • Yang Hwan-Deok (Department of Orthopedic Surgery, College of Medicine, Wonkwang University) ;
  • Kim Hyoung-Jun (Department of Orthopedic Surgery, College of Medicine, Wonkwang University) ;
  • Jeon Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Park Hyun (Department of Infection Biology, College of Medicine, Wonkwang University)
  • 최경민 (원광대학교 의과대학 감염생물학교실) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실) ;
  • 강경화 (원광대학교 의과대학 감염생물학교실) ;
  • 오성수 (원광대학교 의과대학 감염생물학교실) ;
  • 양환덕 (원광대학교 의과대학 정형외과) ;
  • 김형준 (원광대학교 의과대학 정형외과) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 박현 (원광대학교 의과대학 감염생물학교실)
  • Published : 2005.10.01
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Abstract

Catechin products in green tea extract was prepared to investigate antibacterial activity on the pathogenic bacteria. Survival of pathogenic bacteria (MASA - methicillin resistant Staphylocouus aureus, E.coli O157 and S. typhimurium Sal-13) in tryptic soy agar(TSA) containing Catechin products powder incubated at various concentration was evaluated. TSA containing $0{\sim}2%(w/v)$ of Catechin products was inoculated approximately $10^4\;CFU/ml$ of pathogenic bacteria and incubated at $37^{\circ}C$ for 24 hours. The plate counting technique and clear zoon test were used to test survival effect of the Catechin products. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was derived from the survival curves of pathogenic bacteria. S. typhimurium Sal-13 was the most sensitive strain to Catechin products. This result suggested that Catechin products can be used as an effective natural antibacterial agent.

Keywords

References

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