골쇄보(骨碎補) 부탄올 추출물의 U937세포주 세포자멸사에 미치는 효과

Effects on the Apoptosis of U937 cell line by n-butanol Extracts of Drynariae Rhizoma

  • 조성연 (우석대학교 한의과대학 병리학교실) ;
  • 정한솔 (우석대학교 한의과대학 병리학교실)
  • Cho, Sung-Yeon (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University)
  • 발행 : 2006.04.25

초록

To investigate the possible mechanism of Drynariae Rhizoma extracts as a candidate of anti-cancer drug, I examined the effects of Drynariae Rhizoma extracts on the apoptosis of U937 cell line. MTT assay, flow cytometric analysis, SDS-polyacrylamide gel electrophoresis, Western blot analysis, and RT-PCR were performed. Drynariae Rhizoma extracts treatment reduced the cell viablilty of U937 cells in a dose-dependent manner, which was associated with induction of apoptotic cell death. Drynariae Rhizoma extracts treatment also reduced the levels of Bcl-xL anti-apoptotic protein expression and increased the levels of caspase-3, p53, pro-apoptotic protein, in U937 cells. RT-PCR data revealed that the level of bcl-2, bcl-xL mRNA expressions decreased in a dose-dependent manner. These findings suggest that Drynariae Rhizoma extracts may have induction of apoptotic cell death via regulation of several growth regulatory gene products. The abbreviations used are: FBS, fetal bovine serum; PBS, phosphate buffered saline; PI, propidium iodide; OD, optical density; DiOC6, 3,3-dihexyloxa carbcyanine iodide; MTT, 3 [4-5-dimethylthiazol-2-yl] -2-diphenyltetrazolium bromide.

키워드

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