Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Single Dose Toxicity Test of 'Mahwangyounpae-tang' Extract in Male ICR Mouse

마황윤폐탕(麻黃潤肺湯) 추출물의 수컷 ICR 마우스에서 경구 단회투여 독성 평가

  • Jung, Woo-Sik (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Cho, Dong-Hee (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Seo, Yeong-Ho (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Park, Mee-Yeon (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Choi, Hae-Yun (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Jong-Dae (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Jeon, Kwi-Ok (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University)
  • 정우식 (대구한의대학교 한의학과 폐계내과학교실) ;
  • 조동희 (대구한의대학교 한의학과 폐계내과학교실) ;
  • 서영호 (대구한의대학교 한의학과 폐계내과학교실) ;
  • 박미연 (대구한의대학교 한의학과 폐계내과학교실) ;
  • 최해윤 (대구한의대학교 한의학과 폐계내과학교실) ;
  • 김종대 (대구한의대학교 한의학과 폐계내과학교실) ;
  • 전귀옥 (대구한의대학교 한의학과 폐계내과학교실)
  • Published : 2006.04.25
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Abstract

To obtain the 50% lethal dose (LD50), approximated lethal dose (ALD) and approximated target organs of 'Mahwangyounpae-tang' for further study like repeat dose toxicity, genotoxicity and reproductive toxicity, single dose toxicity was tested in male ICR mouse according to KFDA Guideline 1999-61 [KFDA, 1999] at a dosage level of 2,000, 1,000, 500, 250 and $125\;mg/kg/10m{\ell}$. In this study, mortalities, clinical signs, body weight changes and body weight gains, gross findings and weight of principal organs were detected during and/or after 14 days of single dosing. After 2 or 3 days of dosing, 1 or 2 animals in 2,000 and 1,000 mg/kg-dosing groups were died. Excitation and leaping response were observed as test article-treatment related clinical signs. These abnormal signs were restricted to 2,000 and 1,000 mg/kg-dosing groups and they were recovered to normal within 4 days after dosing in case of survivors. A significant decrease of body weight were observed in some periods of observation in 2,000 and 1,000 mg/kg-dosing group from 1 days after dosing compared to those of vehicle control group. A significant decrease of body weight gains were observed in observation periods in 2,000 and 1,000 mg/kg-dosing group compared to those of vehicle control group. Hypertrophy of heart and decoloration of kidney were observed as test article-treatment related gross findings. These abnormal findings were restricted to 2,000 and 1,000 mg/kg-dosing groups. A significant increase of absolute and relative heart and kidney weight were demonstrated in 2,000 mg/kg-dosing groups. LD50 in this study was detected as 2,242.42 mg/kg. ALD in this study was detected as 1,000 mg/kg and the target organ was considered as the heart and kidney.

Keywords

References

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