Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of the Geijibokryunghwan Water Extracts on Stimulus-induced Superoxide Generation and Tyrosyl Phosphorylation in Human Neutrophils

  • Choi, Meung-Hee (Department of Diagnostics and Cardiovascular Medical Research Center, Dongguk University) ;
  • Park, Won-Han (Department of Diagnostics and Cardiovascular Medical Research Center, Dongguk University)
  • Published : 2006.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

A clinical report indicates that 'Geijibokryunghwan(GBH) is very effective in treating thrombosis in those patients who have difficulties with more conventional antithrombotic drugs. The isolation and identification of various compounds from this plant and the same genus have been reported by several groups. However, the pharmaceutical effect of the GBH on superoxide generation in human neutrophils has not been studied. In the present report, we investigated the possibility of using herbal medicine as an alternative therapy. In particular, we studied tremor in antiatheroscleosis. In this report, we shows the GBH extract can be used as a potential atherosclerosis preventive agent in human. The effect of GBH on stimulus-induced superoxide generation and phosphorylation of tyrosine residues of protein in human neutrophils was investigated. In a conclusion, GBH suppressed tyrosine phosphorylase in a dose-dependent manner, and may have pharmacoceutical applications. These data suggest that GBH extracts merits investigation as a potential anti-atherosclerogenic agent in humans.

Keywords

References

  1. Kim, J.G., Kim, C.H., Park, S.Y., Kim, J.S., Choi, J.Y., Kim, C.H., and Park, W.H. An experimental study on the effect of Geijibokryunghwan, Dangguijakyaksan and each constituent herb on inhibition of platelet aggregation. J. of the Korea Institute of Oriental Medical Diagnostics 4, 71-84, 2000
  2. Sugiyama, K., Muteki, T., and Kano, T. The Japanese herbal medicine 'saiko-keishi-to' activates GABAA receptors of rat sensory neurons in culture. Neurosci. Lett. 216, 147-150, 1996 https://doi.org/10.1016/0304-3940(96)13000-7
  3. Ishikawa, T., Funahashi, T., and Kudo. J. Effectiveness of the Kampo kami-shoyo-san (TJ-24) for tremor of antipsychotic-induced parkinsonism. Psychiatry and Clin. Neurosci. 54, 579-684, 2000 https://doi.org/10.1046/j.1440-1819.2000.00756.x
  4. Jiangsu Medical College: Encyclopedia of Chinese Materia Medica, Shanghai Science and Technology Press 1300, 1977
  5. Stossel, T.P. and Phagocytosis. N. Engl. J. Med. 290, 717-723, 1974 https://doi.org/10.1056/NEJM197403282901306
  6. Goldstein, I.M., Roos, D., Kaplan, H.B., and Weissmann, G. Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis. J. Clin. Invest. 56, 1155-1163, 1975 https://doi.org/10.1172/JCI108191
  7. Rossi, F. The O2-forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function. Biochim. Biophys. Acta 853, 65-89, 1986 https://doi.org/10.1016/0304-4173(86)90005-4
  8. Takahashi, R., Edashige, K., Sato, E.F., Inoue, M., Matsuno, T., and Utsumi, K. Luminol chemiluminescence and active oxygen generation by activated neutrophils. Arch. Biochem. Biophys. 285, 325-330, 1991 https://doi.org/10.1016/0003-9861(91)90367-R
  9. McColl, S.R., Dipersio, J.F., Caon, A.C., and Naccache, H.P. Involvement of tyrosine kinase in the activation of human peripheral blood neutrophils by granulocyte-macrophage colony-stimulating factor. Blood 78, 1842-1852, 1991
  10. Watanabe, Y., Sagara, Y., Sugahara, K., and Kodama, H. Iminodipeptides containing proline with C-terminal and N-terminal residues prime the stimulation of human neutrophil superoxide generation by fMLP. Biochem. Biophys. Res. Commun. 205, 758-764, 1994 https://doi.org/10.1006/bbrc.1994.2730
  11. Zhang, J., Sugahara, K., Sagara, Y., Fontana, M., Dupre, S., and Kodama, H. Effect of cystathionine ketimine on the stimulus coupled responses of neutrophils and their modulation by various protein kinase inhibitors. Biochem. Biophys. Res. Commun. 218, 371-376, 1996 https://doi.org/10.1006/bbrc.1996.0065
  12. Kodama, H., Zhang, J. and Sugahara, K. Novel priming compounds of cystathionine metabolites on superoxide generation in human neutrophils. Biochem. Biophys. Res. Commun. 269, 297-301, 2000 https://doi.org/10.1006/bbrc.1999.1970
  13. Lee, S.K. Effect of the Geijibokryunghwan on human hepatocarcinoma cells. A thesis of degree of master, Dongguk Univ., 2001
  14. Kim, J.G., Kim, C.H., Park, S.Y., Kim, J.S., Choi, J.Y., Kim, C.H. and Park, W.H., An experimental study on the effect of Geijibokryunghwan, Dangguijakyaksan and each constituent herb on inhibition of platelet aggregation, The J. of the Korea institute of oriental medical diagnostics, 4, 71-84, 2000
  15. 杜雨茂, 張聯惠, 金匱要略闡釋, 陜西省; 陜西科技, pp 235-252, 1987
  16. 李尙仁外 5人, 漢藥臨床應用, 서울; 成輔社, pp 37, 108, 151-153, 155-156, 267-269, 285, 320-322, 357-361, 1990
  17. 陳可翼外 3人, 血瘀證與活血化瘀硏究, 上海; 上海科技, p 642, 1990
  18. 李克光, 金匱要略, 서울; 아울로스출판사, pp 460-482, 562-569, 1994
  19. Weiss, S.J., Klein, R., Slivka, A. and Wei, M. Chlorination of taurine by human neutrophils. Evidence for hypochlorous acid generation. J. Clin. Invest. 70, 598-607, 1982 https://doi.org/10.1172/JCI110652
  20. Yamamoto, M., Saeki, K., and Utsumi, K. Isolation of salivary polymorphonuclear leukocytes and their stimulation-coupled responses. Arch. Biochem. Biophys. 289, 76-82, 1992
  21. Zhang, J. Sagara, Y. Fontana, M. Dupre, S. Cauallini, D. and Kodama, H. Effect of cystathionine and cystathionine metabolites on the phosphorylation of tyrosine residues in human neutrophils. Biochem. Biophys. Res. Commun. 224, 849-854, 1996 https://doi.org/10.1006/bbrc.1996.1111
  22. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227, 680-685, 1970 https://doi.org/10.1038/227680a0