Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Yeongyupaedog-san on Cytokine Levels of Mouse Th1/Th2 Cells and Anti-allergic Activity in Ovalbumin-sensitized Allergic Inflammation Model

연교적패독산(連翹敗毒散) 물 추출물(抽出物)의 마우스 Th1/Th2 사이토카인 조절(調節)에 의한 항알레르기 효과

  • Khwag, Nyo-Gyu (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Kang, Hee (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Myung, Eu-Gene (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Park, Sung-Min (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Shim, Bum-Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Choi, Seung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
  • 곽노규 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 강희 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 명유진 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 박성민 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 심범상 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 김성훈 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 안규석 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소)
  • Published : 2006.08.25
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Abstract

This study was to evaluate the effect of Yeongyupaedog-san (YGPDS) on mouse Thl and Th2 cells' differentiation and ovalbumin (OVA)-induced allergic inflammation. The proliferation of mouse CD4 T cells and the secretion of Th1/Th2 cytokines under the influence of YGPDS extract were measured as well as the amount of ${\beta}-hexosaminidase$ in RBL-2H3 cells and the levels of $TNF-{\alpha}$ and 1L-6 secretion in Raw264.7 cells. BALB/c mice were orally administered with YGPDS extract and simultaneously inoculated with OVA to induce allergic reaction and measure the level of total IgE, OVA-specific IgE and the production of IFN- g, IL-4, IL-5 by the spleen cells. When mouse CD4 T cell were stimulated with anti-CD3 and anti-CD28 for 48 hours in various concentrations of YGPDS extract, it increased proliferation of CD4 cells by 11% in $100\;{\mu}g/^{ml}$ concentration but it showed an inhibition by 37% at $200\;{\mu}g/^{ml}$ CD4 T cells under Th1/Th2 polarizing conditions for 3 days with YGPDS resulted in mild decrease of IFN- g in Thl cells and significant decrease of IL-4 in Th2 cells at $500\;{\mu}g/^{ml}\;and\;100\;{\mu}g/^{ml}$ by 18% and 21%, respectively. YGPDS extract had a dose-dependent inhibitory effect on antigen-induced release of ${\beta}-hexosaminidase$ in RBL-2H3 cells. Treatment of YGPDS extract on LPS stimulated Raw 264.7 cells showed dose-dependent decrease in TNF-n production. Oral administration of YGPDS extract on OVA-induced allergic mice showed an inhibitory effect on the levels of total serum IgE and OVA-specific IgE by 25% and 34% , respectively. Culture of spleen cells with OVA resulted in significant increase of IFN- g by 44% and significant decrease of IL-4 and IL-5 by 56%, and 24%, respectively. The results show that YGPDS does not strongly induce mouse T cells to transform into Thl or Th2 but it has an anti-allergic effect in vitro, and that it also corrects the unbalance between the reactions of Th cells in allergic diseases.

Keywords

References

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