갈근 추출물의 항산화와 수복효과에 관한 연구

In Vitro Antioxidant and Repaired Effects of the Extracts from Pueraria thunbergiana Roots

  • 신선우 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 신정미 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 육찬남 (원광보건대학 식품영양과) ;
  • 나영순 (건양대학교 미용과학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Shin, Sun-Woo (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Shin, Joung-Mi (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yook, Chan-Nam (Department of Food Nutrition, Wonkwang Health Science College) ;
  • Na, Young-Soon (Department of Beauty Science, Konyang University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 발행 : 2006.08.25

초록

The antioxidative activity was observed in the ethyl acetate extract $(IC_{50},\;119{\pm}0.16\;{\mu}g/mL)$ from Pueraria thunbergiana. The DPPH radical scavenging effect of this extract was comparable with that of synthetic antioxdant, BHA $(IC_{50},\;88.39{\pm}1.1\;{\mu}g/mL){\times}\;10^{-3}\;{\mu}M$. Pueraria thunbergiana extracts against microorganisms were evaluated in terms of the minimum inhibitory concentrations (MIC). In general, C. albicans was stronger antimicrobial activity than the other microorganisms. in vitro the antitoxic activity of extracts of Pueraria thunbergiana on NIH 373 fibroblasts was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) method. Generally, detoxification effect by the extracts of Pueraria thunbergiana increased in proportion to the concentrations. When $10\;{\mu}g/mL$ of the hexane extract of Pueraria thunbergiana was treated it showed the highest antitoxic effects (p<0.01). These results suggest that the crude extract of P. thunbergiana has a potential therapeutic activity.

키워드

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