흑삼의 제조 및 항암효과

Preparation of Black Ginseng and its Antitumor Activity

  • 이지현 (충남대학교 약학대학) ;
  • 신귀남 (충남대학교 약학대학) ;
  • 김의검 (충남대학교 약학대학) ;
  • 신현중 ((주)금산천년홍삼) ;
  • 명창선 (충남대학교 약학대학) ;
  • 오한진 (성균관대학교 의과대학) ;
  • 김동희 (대전대학교 한의과대학 병리학교실) ;
  • 노성수 (대전대학교 한의과대학 본초학교실) ;
  • 조원 (대전대학교 한의과대학 본초학교실) ;
  • 서영배 (대전대학교 한의과대학 본초학교실) ;
  • 박용진 (백남한의원) ;
  • 강철우 (백남한의원) ;
  • 송규용 (충남대학교 약학대학)
  • Lee, Jee-Hyun (College of Pharmacy, Chungnam National University) ;
  • Shen, Gui-Nan (College of Pharmacy, Chungnam National UniversityCollege of Pharmacy, Chungnam National University) ;
  • Kim, Eui-Keom (College of Pharmacy, Chungnam National University) ;
  • Shin, Hyun-Joong (Geum-San Chun-Yun Red Ginseng Co. Ltd.) ;
  • Myung, Chang-Seon (College of Pharmacy, Chungnam National University) ;
  • Oh, Han-Jin (Cheil General Hospital & Women's Health Care Center, Sungkyunkwan University) ;
  • Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejon University) ;
  • Roh, Seong-Soo (Department of Herbalogy, College of Oriental Medicine, Daejon University) ;
  • Cho, Won (Department of Herbalogy, College of Oriental Medicine, Daejon University) ;
  • Seo, Young-Bae (Department of Herbalogy, College of Oriental Medicine, Daejon University) ;
  • Park, Yong-Jin (Baeknam Oriental Medical Hospital) ;
  • Kang, Cheol-Woo (Baeknam Oriental Medical Hospital) ;
  • Song, Gyu-Yong (College of Pharmacy, Chungnam National University)
  • 발행 : 2006.08.25

초록

The purpose of this study is to prepare black ginseng and evaluate its antitumor activity. In order to achieve such aim, 5 year fresh ginsenges were steamed at 95'E for 3 hr in pottery apparatus and dried at $60^{\circ}C$ for 12-36 hr. This process was repeated again nine times in same condition. Among the ginseng saponins in black ginseng, the amount of Ginsenoside $Rg_3$ was examined by HPLC. 10.05 mE of Ginsenoside $Rg_3$ was obtained from 1 g of dried black ginseng prepared. The extract of black ginseng exhibited stronger cytotoxic activity against MCF-1, HT-1080 and Hepa 1C1C7 tumor cell lines in vitro than the extract of red ginseng. Also, the extract of black ginseng exhibited stronger antitumor activity(33%) in BDFl mice bearing Lewis lung carcinoma cells(LLC) than the extract of red ginseng(23%). From these results, it was concluded that Black ginseng had antitumor activity suggesting its application for the prevention and treatment of cancer.

키워드

참고문헌

  1. 이시진, 본초강목, 중국의학대계 41책, 서울, 정담, pp 231- 232, 1998
  2. 제4회 국제인삼심포지움, 1984
  3. 김기환, 정인성, 홍삼다당체의 항암면역증강작용 연구, Korea J. Ginseng Sci, 21(2):78-84, 1997
  4. 서성옥, 정철헌, 소화기계암의 수술후 면역기능에 대한 고려홍삼의 효과, Korea J. Ginseng Sci, 22(1):32-42, 1998
  5. Saito, H. et al. Prooc. 2nd International Ginseng symposium, 109, 1978
  6. Okamura, N., Kobayashi, K., Akaike, A., Yagi, A. Protective effect of ginseng saponins against impaired brain growth in neonatal rats exposed to ethanol. Biol Pharm Bull, 17(2):270-274, 1994 https://doi.org/10.1248/bpb.17.270
  7. 오미숙, 백기주, 전성실, 김규원, 최강주, 김남득, 홍삼 사포닌에 의한 유선상피 및 유선암세포의 분화 유도 효과 연구, Korea J. Ginseng Sci, 24(4):188-195, 2000
  8. 이혜연, 이한수, 홍삼 추출물의 임파구 증식 및 활성 촉진효과, Korea J. Ginseng Sci, 22(1):60-65, 1998
  9. 최영득, 박진아, 최형기, 남기열, 토끼와 흰쥐 음경해면체 이완작용에 홍삼사포닌 분획별 효과, Korea J. Ginseng Sci, 23(1):13-20, 1999
  10. Keum, Y.S., Park, K.K., Lee, J.M., Chun ,K.S., Park, J.H., Lee, S.K., Kwon, H., Surh, Y.J. Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng, Cancer Lett. 150(1):41-48, 2000 https://doi.org/10.1016/S0304-3835(99)00369-9
  11. Rubinstein, L.V., Paull, K.D., Shoemaker, R.H., Simmon, R.M., Skehan, P. and Boyd, M.R. Correlation of screening data generated with a tetrazolium assay (MTT) versus a protein assay (SRB) against a broad panel of human tumor cell lines, Proceedings of the American Association for Cancer Research, 30, p 2418, 1989
  12. Kim, Y., Kim, S.B., You, Y.J., Ahn, B.Z. Deoxypodophyllotoxin; the cytotoxic and antiangiogenic component from Pulsatilla koreana, Planta Med., 68(3):271-274, 2003
  13. Kim, H.S., Lee, E.H., Ko, S.R., Choi, K.J., Park, J.H., Im, D.S. Effects of ginsenosides Rg3 and Rh2 on the proliferation of prostate cancer cells. 27(4):429-435, 2004 https://doi.org/10.1007/BF02980085
  14. Kim, J.H., Lee, J.H., Jeong, S.M., Lee, B.H., Yoon, I.S., Lee, J.H., Choi, S.H., Kim, D.H., Park, T.K., Kim, B.K., Nah, S.Y. Stereospecific effects of ginsenoside Rg3 epimers on swine coronary artery contractions. 29(2):365-370, 2006 https://doi.org/10.1248/bpb.29.365
  15. Cha, H.Y., Park, J.H., Hong, J.T., Yoo, H.S., Song, S., Hwang, B.Y., Eun, J.S., Oh, K.W. Anxiolytic-like effects of ginsenosides on the elevated plus-maze model in mice. 28(9):1621-1625, 2005 https://doi.org/10.1248/bpb.28.1621