Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis

Gel filtration chromatography와 propionic acid/urea polyacrylamide gel electrophoresis를 이용한 봉독 성분의 분리

  • Choi, Young-Chon (Dept. of Acupuncture & Moxibustion, Oriental Medical College, Sangji University) ;
  • Choi, Suk-Ho (Devision of Animal resourses and life science, Sangji University) ;
  • Kwon, Ki-Rok (Dept. of Acupuncture & Moxibustion, Oriental Medical College, Sangji University)
  • 최영권 (상지대학교 한의과대학 침구학교실) ;
  • 최석호 (상지대학교 동물생명자원학부) ;
  • 권기록 (상지대학교 한의과대학 침구학교실)
  • Published : 2006.06.30
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Abstract

Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase $A_2$ and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography. Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom.

Keywords

References

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