Applied Biological Chemistry

  • 제49권4호
  • /
  • Pages.339-342
  • /
  • 2006
  • /
  • 2468-0834(pISSN)
  • /
  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

칸나(Canna generalis)의 뿌리로부터 지질화합물의 분리.동정

Isolation and Identification of Lipids from the Roots of Canna generalis

  • 방면호 (강화군 특화작목연구소) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이대영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 양혜정 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 한민우 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이윤형 (경희대학교 생명과학대학)
  • Bang, Myun-Ho (Ganghwa Agricultural R&D Center) ;
  • Song, Myoung-Chong (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Dae-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Yang, Hye-Jung (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Han, Min-Woo (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Youn-Hyung (Major in Horticultural Biotechnology, Kyung Hee University)
  • 발행 : 2006.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

칸나의 뿌리를 80% MeOH용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획과 n-BuOH 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography로 정제하여 4종의 화합물을 분리하였다. 각 화합물의 화학구조는 NHR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, $\beta$-sitosterol(1), linoleic aicd methyl ester(2), monoglycosyldiglyceride(3), daucosterol(4)으로 동정하였다. 이 화합물들은 칸나에서 처음으로 분리되었다.

Canna generalis was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. from the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated silica gel and ODS column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as $\beta$-sitosterol(1), linoleic acid methyl ester(2),1-O-oleoyl-2-O-linoleoyl-3-O-$\beta$-D-galactopyranosyl-sn-glycerol(3), and daucosterol(4). They were the first to be isolated from Canna generalis.

키워드

참고문헌

  1. Lee, T. B. (2003) In Coloured Flora of Korea, Hyang Mun Sa, Seoul, Korea
  2. Jun, D. L. and Qin, Z. L. (2002) Study on the stability of red pigment from Canna flowers. Food Sci. Biotechnol. 7, 40-42
  3. Qiang, Z. Z. (2005) Technique of extraction on the red pigment from flower of Canna india. Appl. Chem. Ind. 34, 54-62
  4. Kim, D. H., Song, M. Ch., Han, K. M., Bang, M. H., Kwon, B. M., Kim, S. H., Kim, D. K., Chung, I. J., Park, M. H., Baek, N.-I. (2004) Development of biologically active compounds from edible plant sources-X. Isolation of lipids from the flower of Campsis grandiflora K. Schum. and their inhibitory effect on FPTase. J. Korea Soc. Appl. Biol. Chem. 47, 357-360
  5. Song, M. Ch., Hong, Y. H., Kim, D. H., Kim, D. K., Chung, I. S., Lee, Y. H., Kim, S. H., Park, M. H., Kim, I. H., Kwon, B. M. and Baek, N.-I. (2003) Development of biologically active compounds from edible plant sources-VI. Isolation of sterol compounds from the aerial parts of garland (Chrysanthemum coronarium L.). J. Korea Soc. Agric. Chem. Biotechnol. 46, 376-379
  6. Zheng, C. J., Yoo, J. S., Lee, T. G., Cho, H. Y., Kim, Y. H. and Kim, W. G. (2005) Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids. FEBS Lett. 579, 5157-5162 https://doi.org/10.1016/j.febslet.2005.08.028
  7. Wakako, T. (1995) Conformation analysis of glycerides by nuclear magnetic resonance. Phytochemistry 76, 93-97
  8. Ahn, E. M., Lee, M. H., Rho, Y. D., and Baek N.-I. (1998) Isolation of daucosterol from the rice hull of Oryza sativa L. J. Korea Soc. Agric. Chem. Biotechnol. 41, 486-488
  9. Hyun, J. W., Yang, Y, M., Sung, M. S., Chung, H. S., Paik, W. H., Kang, S. S., Park, J. G. (1996) The cytotoxic activity of sterol derivatives from Pulsatilla chinensis regal. J. Korean Cancer 28, 145-150
  10. Carlos, R. P., Matias, G., Jose, C. G., Marta, E. S. and Carlis, E. T. (2005) Insecticidal and antifeedant effects of Junellia aspera (Verbenaceae) triterpenes and derivatives on Sitophilus oryzae. J. Stored Prod. Res. 41, 433-443 https://doi.org/10.1016/j.jspr.2004.07.001