Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Evaluation of human exposure to polychlorinated biphenyls using human serum by congener-specific analysis

혈액 중 PCBs 이성질체별 분석에 의한 인체 노출 평가

  • Park, Hyo-Keun (School of Environmental Science & Engineering, Pohang University of Science and Technology) ;
  • Lee, Se-Jin (School of Environmental Science & Engineering, Pohang University of Science and Technology) ;
  • Jin, Guang-Zhu (School of Environmental Science & Engineering, Pohang University of Science and Technology) ;
  • Kang, Jung-Ho (School of Environmental Science & Engineering, Pohang University of Science and Technology) ;
  • Baek, Song-E (School of Environmental Science & Engineering, Pohang University of Science and Technology) ;
  • Chang, Yoon-Seok (School of Environmental Science & Engineering, Pohang University of Science and Technology)
  • 박효근 (포항공과대학교 환경공학부) ;
  • 이세진 (포항공과대학교 환경공학부) ;
  • 김광수 (포항공과대학교 환경공학부) ;
  • 강정호 (포항공과대학교 환경공학부) ;
  • 백송이 (포항공과대학교 환경공학부) ;
  • 장윤석 (포항공과대학교 환경공학부)
  • Received : 2005.11.02
  • Accepted : 2006.01.23
  • Published : 2006.02.27
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Abstract

Blood serum concentrations of polychlorinated biphenyls (PCBs) were measured in employees who worked at a municipal solid waste incinerator (MSWI), members of residential community who lived near the MSWI (<0.3km) and members of residential community lived far from the MSWI (>10 km). Human blood serum samples were analyzed for all PCB congeners using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). The mean levels of total PCBs and dioxin-like PCBs in 87 serum samples were 242.77 ng/g lipid and 8.83 TEQ pg/g lipid, respectively. The PCB homologue profiles showed that penta-, hexa-, hepta-chlorinated biphenyls contributed more than 80% of the total PCBs concentration. The most abundant congeners were PCB153, PCB138, PCB180, PCB187, PCB118. A statistical analysis was performed to determine whether there were significant correlations between PCB concentrations and specific variables such as age, gender, smoking habits, occupation, BMI (Body Mass Index) and time of residence. As a result, the age was found to be strongly correlated with serum PCB concentrations. In addition, there were strong correlations between total PCBs and PCB153 (r=0.93, p<.0001), dioxin-like PCBs and PCB118 (r=0.98, p<.0001). So these two congeners are satisfactory indicators for total PCB concentrations and dioxin-like PCBs in human blood respectively.

고분해능 가스크로마토그래피/고분해능 질량분석기를 이용하여 자원회수시설 근로자와 자원회수시설 주변 지역 주민(<0.3 km), 자원회수시설과 관련이 없는 지역주민(>10 km)의 혈액으로부터 PCBs의 모든 이성질체를 분석하였다. 전체 87개의 혈액시료 중에서 PCBs의 평균농도와 다이옥신 유사 PCBs의 평균농도는 각각 242.77 ng/g lipid, 8.83 TEQ pg/g lipid 이었다. 5, 6, 7 염소 치환 동족체가 전체 농도의 80%이상을 차지하였으며 가장 많은 농도 분포를 보이는 이성질체는 PCB153, PCB138, PCB180, PCB187, PCB118이었다. PCBs의 농도와 설문을 통해 조사된 나이, 성별, 흡연여부, 직업, BMI (Body Mass Index), 거주기간 등과 같은 변수와의 유의한 상관관계를 알기위해 통계분석을 한 결과 나이만이 혈액 내 PCBs농도와 상관관계가 있었다. 또한, 총 PCBs 농도와 PCB153 농도(r=0.93, p<.0001), dioxin-like PCBs와 PCB118의 농도(r=0.98, p<.0001)는 상관관계가 매우 높았다. 따라서 이 두 개의 이성질체는 인체 혈액에서 각각 PCBs농도와 dioxin-like PCBs의 농도의 지표물질(indicator)이 될 수 있다.

Keywords

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