수종의 한약 복합 추출물의 항균, 항염 및 항알레르기 효과에 대한 실험적 연구

Study on the Anti-microbacterial Activity, Anti-inflammatory and Anti-allergic Effects of Several Herb-Extract

  • 김진만 (원광대학교 한의과대학 한방안이비인후피부과) ;
  • 오한철 (원광대학교 한의과대학 한방안이비인후피부과) ;
  • 송성필 (원광대학교 한의과대학 한방안이비인후피부과) ;
  • 김남권 (원광대학교 한의과대학 한방안이비인후피부과) ;
  • 황충연 (원광대학교 한의과대학 한방안이비인후피부과)
  • Kim Jin-Man (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University) ;
  • Oh Han-Cheol (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University) ;
  • Song Seong-Pil (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Nam-Kwen (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University) ;
  • Hwang Chung-Yeon (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University)
  • 발행 : 2006.02.01

초록

Herbal mixture water extract of (Chrysanthemum morifolium, Portulaca oleracea, Sanguisorba officinalis, Sophora flavescens, Phellodendron amurense, Cnidium monnieri) which exhibit several beneficial effects including acne and skin diseases, was tested for anti-microbial activity and anti-inflammation effects. The herbal mixture extract showed antimicrobial activity against Stapylococcus epidermis, and Propionibacterium acne. The growth of Stapylococcus epidermis, and Propionibacterium acne was inhibited completely by addition of 1.0% of the extract. Also in the present study we examined the mixture extract on compound 48/80 induced allergy and LPS induced cyclooxygenase-2(COX-2) gene expression in RAW 264.7 macrophage. The results indicated the ear swelling and histamine release induced by compound 48/80 were dose-dependently reduced, ranging 28-60%, and 48-72% , respectively. Furthermore the extract inhibited the expression of LPS-induced COX-2 proteins and mRNAs without an appreciable cytotoxic effects on RAW 264.7 cells. The LPS-induced cytokine gene expression including IL-$1{\beta}$, TNF-$\alpha$, and IL-6 were dose-dependently suppressed by the mixture extract. Based on these results, it is concluded that the herbal mixture water extract can be applied to the acne and skin diseases therapy.

키워드

참고문헌

  1. 金圭烮. 黃帝內經素問校注滙粹. 서울, 일중사, 上 pp 379, 436-447, 中 pp 284-288, 1998
  2. 鄭遇悅. 韓方病理學. 이리, 서울공판사. pp 5-34, 1983
  3. Kim, E.J., Jin, H.K., Kim, K.,Lee, H.Y., Lee, S.Y.,. Lee, .K.R., Zee, O.P., Han, J.W., Lee, H.W. Suppression by a sesquiterpene lactone from Carpesium divaricantum of inducible nitric oxide synthase by inhibiting nuclear factor-kB activation. Biochem. Pharmarol. 61, 903-910, 2001 https://doi.org/10.1016/S0006-2952(01)00538-X
  4. 신연상. 유백피의 항염 및 조직재생에 대한 실험적 연구. 대한외관과학회지 14(1):129-153, 2001
  5. 신민교. 임상본초학. 영림사. p 400, 405, 785, 1997
  6. 范成玉외 2인. 中藥治愈. 応激. 変態性 頑固濕疹 5例. 四川中醫. 19(10):58-59, 2001
  7. 대한피부과학회 교과서 편찬위원회. 피부과학. 서울, pp 161-166, 461-464, 2001
  8. Peter, G. Smith, A.L. Group A Streptococcal infections of the skin and pharynx. N Engl J Med. 297, 311-315, 1977 https://doi.org/10.1056/NEJM197708112970606
  9. 홍석훈, 노석선. 청상방풍탕가미가 면포에 미치는 영향. 대한안이비인후과학회지 15(1):315-335, 2002
  10. 최은규, 노석선. 용담사간탕가미방이 염증치료 및 예방에 미치는 영향에 대한 연구. 17(2):39-47, 2004
  11. Kanemoto, T., Kasugai, T., Yamatodani, A., Ushio, H., Mochizuki, T.K., Kimura, M., Nishimura, M., Kitamura, Y. Supernormal histamine release and normal cytoxic activity of beige rat mast cells with giant granules. Int. Arch. Allergy Immunol. 100, 99-106, 1993 https://doi.org/10.1159/000236395
  12. Yurt, R.W., Leid, R.W., Austen, K.F. Native heparin from rat peritoneal mast cells. J. Biol. chem. 252, 518-521, 1977
  13. Shore, P.A., Burkhalter, A., Cohn, V.H. A method for fluorometric assay of histamine in tissues. J. Pharmacol. Exp. Ther. 127, 182-186, 1959
  14. Kawabata, T.T., Babcock, L.S. Measurement of murine ovalbumin-specific IgE by a rat basophil leukemia cell serotonin release assay. J. Immunol. Method. 162, 9-15, 1993 https://doi.org/10.1016/0022-1759(93)90401-R
  15. Katayama, S., Shinoya, H., Ohtake, S. A new method for extraction of extravasated dye in the skin and the influence of fasting stress on passive cutaneous anaphylaxis in gunea pigs and rats. Microbiol. Immunol. 22, 89-101, 1978 https://doi.org/10.1111/j.1348-0421.1978.tb00352.x
  16. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65, 55-63, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  17. 이은방 외. 사상자의 항염증작용. 생약학회지 29(4):384-390, 1998
  18. 박광균. 구강생화학 12장 면역과 염증. 군자출판사. pp 318-325, 1999
  19. 巢元方. 巢氏諸病源候論. 台中, 昭人出版社. pp 10-11, 1982
  20. 戴思恭. 證治要訣. 傳方. 中醫免疫思想急成就. 중의잡지 11(25):56, 1884
  21. 王琦 외. 黃帝內經 素問今釋. 서울, 成輔社. p 14, 1983
  22. 吳謙 외. 醫宗金鑑. 台北, 大中國圖書公司. p 125, 1981
  23. 柳志允. 外科 皮膚科의 辨證論治. 부천, 서원당. pp 232-233, 1987
  24. 李用粹. 證治彙補. 台北, 선풍출판사. p 229, 1977
  25. 宋太宗命撰. 太平聖惠方 券四十. 서울, 윤성사. pp 1207-1219, 1979
  26. 李梴. 醫學入門. 서울, 윤성사. 1983: 405-6, 1983
  27. 龔延賢. 萬病回春 券下. 서울, 행림서원. 1972: 9-10, 1972
  28. 許俊. 東醫寶鑑. 서울, 남산당. p 209, 239, 284, 1976
  29. 홍남두 외. 지실의 항염증 성분연구. 생약학회지. 1991
  30. 지형준. 본초에서 건강식품의 개발. 한국식품개발연구원. 7(3):42, 1994
  31. Nam, S.H., Choi, J.S., Jang, D.S., Choi, S.U., Yang, M.S. Effects of sesquiterpene lactone isolated from Chrysanthemum boreale M. against sarcoma 180 implanted in ICR mice. J.Korean Soc. Food Sci. Nutr. 26, 144-147, 1997
  32. Bastow, K.F., Bori, I.D., Fukushima, Y., Kashiwada, Y., Tanaka, T., Nonaka, G., Nishioka,, I., Lee, K.H. Inhibition of DNA topoisomerases by sanguiin H-6, a cytotoxic dimeric ellagitanin from Sanguisorba officinalis. planta Med. 59, 240-245, 1993 https://doi.org/10.1055/s-2006-959659
  33. 朴捅基 외. 薏苡仁과 苦蔘의 抗酸化 作用에 關한 硏究. 서울, 대한본초학회지 15(2):57-68, 2000
  34. 難波恒雄. 和漢藥百科圖鑑(I). 大阪, 保育社. pp 230-231, 1993
  35. 이건겁 외 6인. 진단미생물학(3판). 서울, 고려의학. pp 322-323, 359-374, 1999
  36. McGinley, K.J. et al. Regional variations in density of cutaneous propionibacteria: correlation of Propionobacterium acnes populations with sebaceous secretion, J Clin Microbiol. 12, 672-675, 1980
  37. 최승만 외 4인. Propionibacterium acnes에 대한 천연물의 항균효과 검색. 대한약학회지 42, 89-94, 1998
  38. Faergemann J. Pityrosporum species as a cause of allergy and infection. Allergy 54, 413-419, 1999 https://doi.org/10.1034/j.1398-9995.1999.00089.x
  39. 康秉秀. 한방임상 알레르기. 서울, 성보사. pp 196-201, 22-23, 61-68, 1988
  40. Metcalfe, D.D., Baram, D., Mekori, Y.A. Mast cells. Physiol. Rev. 77, 1033-1079, 1977
  41. Okayama, Y.,Petit-Frere, C., Kassel,O., Semper, A., Quint, D., Tunon-de-Lara, M.J. Bradding, P., Holgate, S.T., Church, M.K.. Ig E-independent expression of mRNA for IL-4 and IL-5 HUMAN LUNG MAST CELLS. J.Immunol. 155, 1796-808, 1995
  42. Moller, A., Heng, B.M., Grutzkau, A., Lippert, U., Aragane, Y., Schwarz, T., Kruger-Krasagakes, S. Comparative cytokine gene expression: regulation and release by human mast cells. Immunology. 93, 289-295, 1998 https://doi.org/10.1046/j.1365-2567.1998.00425.x
  43. Holgate, S.T., Robinson, C., Church, M.K. Allergy, Pincples and Pactice. 3rded, Mosby, St. Louis. pp 135-136, 1988
  44. 서영준. 발암과정에 있어서 Cyclooxygenase-2의 역할 및 그 저해를 통한 화학 암예방. 분자생물학뉴스. 13, pp 8-17, 213-347, 2002
  45. Baeuerle, P.A. The inducible transcription activator NF-$\kappa$B Regulation by distinct subunits. Biochim. Biophys. Acta. 1072, 63-70, 1991
  46. Benjamin, R.V., Shijun, Y., James, J.L. Induction of proinflammatory cytokines by a soluble factor of Propionibacterium acnes. Infection and Immunity 63, 3158-3165, 1995