한국환경성돌연변이발암원학회지 (Environmental Mutagens and Carcinogens)

한국환경성돌연변이발암원학회 (Korean Environmental Mutagen Society)
권호 표지

홍합 [Mytilus coruscus]으로부터 항균활성 펩타이드의 정제

Purification of a Antimicrobial Peptide from the Marine Mussel, Mytilus coruscus

  • 김인혜 (신라대학교 공과대학 제약공학과) ;
  • 김진욱 (신라대학교 공과대학 제약공학과) ;
  • 이재화 (신라대학교 공과대학 제약공학과)
  • Kim In-Hae (Department of Pharmaceutical Engineering, College of Engineering, Silla University) ;
  • Kim Jin-Wook (Department of Pharmaceutical Engineering, College of Engineering, Silla University) ;
  • Lee Jae-Hwa (Department of Pharmaceutical Engineering, College of Engineering, Silla University)
  • 발행 : 2006.03.01
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초록

흥합 (Mytilus coruscus) 추출물 RM 60을 사용하여 E. coli D31을 대상으로 항균활성 펩타이드를 분리 정제하였다. 정제한 펩타이드는 MALDI-TOF Mass spectrometry를 사용하여 분자량을 측정한 결과, 1464.92 Da로 나타났으며 Edman 분해법을 이용한 peptide sequencer를 사용하여 일차구조를 분석 중에 있다.

Antimicrobial peptides (AMPs) play an important role in this response by rapidly killing invading microorganisms. In this study antimicrobial peptide has been isolated from acidified whole body extract of a bivalve mollusk, the marine mussel (Mytilus coruscus). This peptide purified to homogeneity by gel-filtration and reversed-phase high performance liquid chromatography. The molecular weight was 1464.92 Da, determined by MALDI-TOF Mass spectrometry. In addition to growth inhibition of Escherichia coli D31.

키워드

참고문헌

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