Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effect of Hwansodan in Serum and Glucose Deprivation Induced-apoptotic Death of PC12 Cells Via Ho-1 Expression

영양혈청 결핍성 PC12 세포고사에서 HO-1의 발현 증가를 통한 환소단의 보호 효과

  • Jung, Jae-Eun (Department of internal Medicine, School of Oriental Medicine) ;
  • Kim, Jin-Kyung (Department of Microbiology, School of Medicine, Wonkwang University) ;
  • Kang, Baek-Gyu (Department of internal Medicine, School of Oriental Medicine) ;
  • Park, Chan-Ny (Department of Microbiology, School of Medicine, Wonkwang University) ;
  • Park, Rae-Kil (Department of Microbiology, School of Medicine, Wonkwang University) ;
  • Moon, Byung-Soon (Department of internal Medicine, School of Oriental Medicine)
  • 정재은 (원광대학교 한의과대학 심계내과학교실) ;
  • 김진경 (원광대학교 의과대학 미생물학교실) ;
  • 강백규 (원광대학교 한의과대학 심계내과학교실) ;
  • 박찬희 (원광대학교 의과대학 미생물학교실) ;
  • 박래길 (원광대학교 의과대학 미생물학교실) ;
  • 문병순 (원광대학교 한의과대학 심계내과학교실)
  • Published : 2006.12.25
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Abstract

The water extract of Hwansodan has been traditionally used for treatment of ischemic brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of Hwansodan rescues cells from neurodegenerative disease. PC12 pheochromocytoma cells have been used extensively as a model for studying the cellular and molecular mechanisms of neuronal cell damages. Under deprivation of growth factor and ischemic injury, PC12 cells spontaneously undergoes apoptotic cell death. Serum and glucose deprivation markedly decreased the viability of PC12 cells, which was characterized with apparent apoptotic features such as membrane blebbing as well as fragmentation of genomic DNA and nuclei. However, the aqueous extract of Hwansodan significantly reduced serum and glucose deprivation-induced cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Pretreatment of Hwansodan also ingibited the activation of caspase-3, in turn, degradation of ICAD/DFF45 was completely abolished in serum and glucose deprivated cells. Furthermore, pretreatment of Hwansodan obviously increased heme oxygenase 1 (HO-1) expression in PC12 cells. Taken together, the data suggest that the protective effects of Hwansodan against serum and glucose deprivation induced oxidative injuries may be achieved through the scavenging of reactive oxygene species accompanying with HO-1 induction.

Keywords

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