Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-cancer Effects of Bujeonghangam-tang on Human Neuroblastoma Cell Line LAN5

인간 신경모세포종 세포주 LAN5에 대한 부정항암탕(扶正抗癌湯)의 항종양효과

  • Cho, Young-Kee (Wonkwang University Jeonju Oriental Medicine Hospital) ;
  • Lee, Seong-Kyun (Wonkwang University Jeonju Oriental Medicine Hospital) ;
  • Lee, Jung-Sup (Wonkwang University Jeonju Oriental Medicine Hospital) ;
  • Nam, Sang-Kyu (Wonkwang University Jeonju Oriental Medicine Hospital) ;
  • Jeong, Hyun-Ae (Wonkwang University Iksan Oriental Medicine Hospital) ;
  • Moon, Goo (Wonkwang University Jeonju Oriental Medicine Hospital) ;
  • Moon, Mi-Hyun (Wonkwang University Jeonju Oriental Medicine Hospital)
  • 조영기 (원광대학교 전주 한방병원) ;
  • 이성균 (원광대학교 전주 한방병원) ;
  • 이정섭 (원광대학교 전주 한방병원) ;
  • 남상규 (원광대학교 전주 한방병원) ;
  • 정현애 (익산 한방병원) ;
  • 문구 (원광대학교 전주 한방병원) ;
  • 문미현 (원광대학교 전주 한방병원)
  • Published : 2006.12.25
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Abstract

Bujeonghangam-tang(BHT) has been used as an anticancer agent in oriental medicine, but the mechanism by which it induces cell death in cancer cells is still unclear. To investigate cell death mechanism by BHT in cancer cells, the activities of apoptosis signaling pathway were tested in human neuroblastoma cell line LAN5. Viability of LAN5 cells was markedly decreased by treatment of the water extract of BHT in a dose-dependent manner. BHT induced cell death was confirmed as apoptosis characterized by chromatin condensation. We tested whether the water extract of BHT affects the anti-apoptotic protein such as Bcl-2 and Bcl-XL, and the pro-apoptotic protein such as Bax. Both Bcl-2 and Bcl-XL were gradually decreased but Bas was increased in a time-dependent manner after the addition of the water extract of BHT. Cleavage of Bid by activation of caspase-8 protease was also observed in LAN-5 cells by the treatment of the water extract of BHT. Taken together, these results suggest that the water extract of BHT exerts anti-cancer effects on human neuroblastoma LAN-5 cells by inducing the apoptotic death via down-regulation of anti-apoptotic proteins such as Bcl-2 and Bcl-XL, up-regulation of pro-apoptotic protein such as Bax, and activation of intrinsic caspase cascades.

Keywords

References

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