Inhibitory Effect of Extract of Chelidonii Harba on Sebum Synthesis

백굴채(白屈菜) 추출물의 피지생성 억제효과

  • Choi, Doo-Ho (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Park, Si-Jun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Kim, Ho-Min (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • No, Seong-Taek (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Yoo, Il-Soo (Department of Bio & Applied Chemistry, Iksan national College) ;
  • Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Lim, Kyu-Sang (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
  • 최두호 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 박시준 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김호민 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 노성택 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 유일수 (익산대학 생명응용화학계열) ;
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 임규상 (원광대학교 한의과대학 안이비인후피부과교실) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2006.12.25

Abstract

Sebum is secreted due to the effect of androgen, which start to be secreted at puberty. Androgens have profound effects on the physiology of the sebaceous gland, Using the human sebocyte cell line SZ95, we investigated the inhibitory effect of Chelidonii Harba (CH) on the subum production. Our results showed that numerous cytoplasmic lipid droplets were examined by Oil red staining and lipid droplets were increased markedly by testosterone. Cell viability was dose-dependently decreased by CH as compared with untreated cells, while total lipid content and cholesterol slightly were increased by CH. Testosterone significantly stimulated the synthesis of total lipid and the synthesis of specific sebaceous lipids such as cholesterol and triglyceride. Combined treatment with CH and testosterone resulted in a lower lipid synthesis than with testosterone alone. Especially cholesteol content was reduced by combined treatment with CH and testosterone. These results indicate that CH inhibits the testosterone-induced lipid synthesis in SZ95 cells and acts antagonistically to androgen at the cellular level.

Keywords

References

  1. 신민교, 임상본초학, 서울, 영림사 p 723, 1997
  2. 대한피부과학회 간행위원회: 피부과학, 서울, 여문각, p 383, 1994
  3. Nicolaides, N. Human skin furface lipids-orgin, composition and possible function, in Montagna W, Ellis RA, Silver AF(eds) : The Sebaceous glands, Advances in Biology of Skin. Vol. 4, Pergamon Press, London, pp 167-187, 1963
  4. Thiboutot, D. Regulation of human sebaceous glands. J. Investigative Dermatology 123:1-12, 2004 https://doi.org/10.1111/j.1523-1747.2004.t01-2-.x
  5. De Raeve, L., De Schepper, J., Smitz, J. Prepubertal acne : A cutaneous marker of androgen excess? J Am Acad Dermatol 32:181-184, 1995 https://doi.org/10.1016/0190-9622(95)90122-1
  6. Wu, S.F., Klinder, B.N., Trunnell, T.N., Fulton, J.E. Role of anxiety and anger in acne patients: a relationship with the severity of the disorder, J Am Acad Dermatol 18(2Pt 1):325-333, 1988 https://doi.org/10.1016/S0190-9622(88)70047-X
  7. 최상진 외, 애기똥풀의 세포독성 성분. 생약학회지 32(1): 10-14, 2001
  8. 소진백 외, 백굴체의 항암 효과에 관한 연구. 대한본초학회지 12(2), 1997
  9. 김용득 외, 백굴체 수침, 전탕액 투여 및 침자가 Indomethacin으로 유발된 백서의 위궤양에 미치는 영향. 대한침구학회지 12(2), 1995
  10. 김민수 외, 애기똥풀 과실의 Alkaloid 성분. 생약학회지 31(4): 390-393, 2000
  11. Gollnick, H., Zouboulis, C.C., Akamatsu, H., Kurokawa, I., Schulte, A. Pathogenesis and pathogenesis related treatment of acne, J Dermatol 18:489-499, 1991 https://doi.org/10.1111/j.1346-8138.1991.tb03122.x
  12. Akamatsu, H., Zoubolis, C.C., Orfanos, C.E. Spironolactone directly inhibits proliferation of cultured human facial sebocytes and 5$\alpha$-Dihydrotestosterone in vitro, J Invest Dermatol 100:660-662, 1993 https://doi.org/10.1111/1523-1747.ep12472325
  13. 문연자, 김윤석, 권강주, 이희섭, 노성택, 김양진, 이장천, 우원홍. 피지선세포에서 Retinoic Acid의 피지생성억제효과. 동의생리병리학회지 18(5):1317-1321, 2004
  14. Mosmann, T. Rapid colorimetric assay for the cellular growth and survival; application to proliferation and cytotoxic assay. J. Immun. Methods. 65:55, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  15. 대한피부과학회 간행위원회: 피부과학, 서울, 여문각, p 383, 1994
  16. 巢元方, 諸病源候論, 臺中, 昭仁出版社, pp 10-11, 1958
  17. 채병윤, 한방외과, 서울, 고문사, p 310, 1975
  18. 粱劍輝, 常見皮膚病中醫治療簡便, 北京, 人民衛生出版社, p 57, 1986
  19. 陳實功, 外科正宗, 上海, 上海科學技術出版社, p 289, 1989
  20. 안덕균, 한국본초도감, 서울, 교학사, 1998
  21. 顧伯康, 中醫外科學, 人民衛生出版社, 北京, pp 305-307, 1987
  22. 廷賢, 萬病回春, 北京, 人民衛生出版社, p 265, 1988
  23. 上海中醫學院, 中醫外科學, 香港, 商務印書官, p 43, 1979
  24. 上海中醫學院, 中醫外科學講義, 香港, 中藥衛生出版社, pp 230-231, 1966
  25. 안성구, 이승헌, 박윤기: 흔히 보는 피부질환, 서울, 고려의학, p 59, 1993
  26. 吳謙 외, 醫宗金鑒(下), 서울, 대성출판사, p 53, 1983
  27. 許俊, 東醫寶鑑, 서울, 南山堂, p 209, 239, 284, 1976
  28. 王肯堂, 六科准繩(외과), 서울, 대성문화사, pp 237-239, 1982
  29. 上海中醫學院, 中醫學基礎, 香港, 商務印書官, p 43, 1979
  30. 瘀血綜合科學硏究會, 瘀血硏究, 東京, p 17, 23, 1982
  31. 徐宜厚, 王保方: 皮膚病中醫診療學, 北京, 人民衛生出版社, pp 104-108, 1997
  32. 龔信纂 외, 古今醫鑑, 南昌, 江西科學技術出版社, pp 233-234, 1990
  33. 龔延賢, 萬病回春 卷下, 서울, 杏林書院, pp 9-10, 1972
  34. 龔延賢, 壽世保元, 北京, 人民衛生出版社, pp 421-422, 1996
  35. 沈金鰲, 雜病源流犀燭, 北京, 中國中醫藥出版社, p 346, 1994
  36. 樓英, 醫學綱目 卷二十, 臺南, 北一出版社, pp 4-5, 17, 1984
  37. 羅天益, 衛生寶鑑, 香港, 商務印書館, pp 126-127
  38. 申天浩, 病症診治, 서울, 成輔社, pp 592-594, 1990
  39. 中醫硏究院, 中醫症狀鑑別診斷學, 北京, 人民衛生出版社, pp 656-657, 1981
  40. 顧伯華 외, 實用中醫外科學, 上海, 上海科學技術出版社, pp 535-536, 1985
  41. 吳謙 외, 醫宗金鑒, 臺北, 大中國圖書公司, p 125, 1981
  42. 蔡炳允, 漢方外科學, 서울, 高文社, pp 90-91, 1975
  43. 李用粹,: 證治彙補, 臺北, 文光圖書有限公司, p 217, 1979
  44. 陳實功, 外科正宗, 北京, 人民衛生出版社, p 255, 1964
  45. 朴炳昆, 增補 漢方臨床四十年, 서울, 大光文化社, pp 460-461, 1971
  46. Leyden, J.J., Shalita, A. Rational therapy for acne vularis. an update on topical treatment. J Am Acad Dermatol 15:907-915, 1986 https://doi.org/10.1016/S0190-9622(86)70250-8
  47. Kligman, A.M., Fulton, J.E., Plewig, G. Topical vitamin A acid in acne vularis. Arch Dermato 199:469-476, 1969
  48. Thomsen, R.J., Stranieri, A., Knuson, D. et al. Topical clindamycin treatment of acne. Arch Dermatol 116:1031-1034, 1980 https://doi.org/10.1001/archderm.116.9.1031
  49. 홍석훈, 淸上防風湯加味가 면포에 미치는 실험적 연구. 대전대학교 대학원 석사논문. 1998
  50. 서형식, 升麻胃風湯加味가 면포에 미치는 실험적 연구. 대전대학교 대학원 석사논문. 1998
  51. 김성범, 김경준, 連翹敗毒散加味方이 염증상태의 면포에 미치는 영향, 대한안이비인후피부과학회지, 15(1):50-62, 2001
  52. 두인선, 서윤정, 우원홍, 오한철, 박민철, 황충연, 임규상, 김남권, 5% 魚腥草 추출물 수용액의 외용요법이 여드름에 미치는 임상적 연구. 동의생리병리학회지 18(2):612-620, 2004
  53. Downing, D.T. Synthesis and composition of surface lipids of lipids of the skin. J Invest Dermatol 62:228-234, 1974 https://doi.org/10.1111/1523-1747.ep12676793
  54. Zouboulis, C.C., Krieter, A., Gollnick, H., Mischke, D., Orfanos, C. Progressive differentiation of human sebocytes in vitro is characterized by increasing cell size and altering antigen expression and is regulated by culture duration and retinoids, Exp Dermatol. 3(4):151-1601, 1994 https://doi.org/10.1111/j.1600-0625.1994.tb00271.x
  55. Darley, C.R., Moore, J.W., Besser, G.M., Munro, D.D., Edwards, C.R., Rees, L.H., Kirby, J.D. Androgen status in women with late onset or persistent acne vulgaris, Clin Exp Dermatol 9:28-35, 1984 https://doi.org/10.1111/j.1365-2230.1984.tb00751.x
  56. Schmidt, J.B., Spona, J. Hormone receptors in normal skin and acne, Endocrinol Exp 17:137-144, 1983
  57. Sperling, L.C., Heimer, W.L. Androgen biology as a basis for the diagnosis and treatment of androgenic disorders in women. I, J Am Acad Dermatol 28(6):901-916, 1993 https://doi.org/10.1016/0190-9622(93)70129-H
  58. Strauss, J.S., Kligman, A.M., Pochi, P.E. The effect of androgens and estrogen on human sebaceous glands. J Invest Dermatol 39:139-155, 1962 https://doi.org/10.1038/jid.1962.22
  59. Pochi, P.E., Strauss, J.S., Mescon, H. The role of adrenocortical steroids in the control of human sebaceous gland activity. J Invest Dermatol 41:391-399, 1963 https://doi.org/10.1038/jid.1963.73
  60. Schmidt, J.B., Spona, J. Hormone receptors in normal skin and acne, Endocrinol Exp 17:137-144, 1983
  61. Sansone, G., Reisner, R.M. Differential rates of conversion of testosterone to dihydrotestosterone in acne and in normal human skin-A possible pathogenic factor in acne, J Invest Dermatol 56:366-372, 1971 https://doi.org/10.1111/1523-1747.ep12261252
  62. Webster, G.F. Inflammation in acne vulgaris, J Am Acad Dermatol 33:247-253, 1995 https://doi.org/10.1016/0190-9622(95)90243-0
  63. Geiger, J.M. Retinoids and sebaceous gland activity, Dermatology 191:305-310, 1995 https://doi.org/10.1159/000246581
  64. Pochi, P.E., Shalita, A.R., Strauss, J.S. et al. Report of the Consensus conference on acne classification. J Am Acad Dermatol 24:495-500, 1990
  65. Emerson, G.W., Strauss, J.S. Acne and acne care a trend survey. Arch Dermatol. 105:407-411, 1972 https://doi.org/10.1001/archderm.105.3.407
  66. Greene, R.S., Downing, D.T., Pochi, P.E., et al, Anatomical variation in the amount and composition of human skin surface lipids. J invest Dermatol. 54:240-247, 1970 https://doi.org/10.1111/1523-1747.ep12280318