Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Induction of Apoptotic Cell Death by Methanol Extract of Houttuynia cordata Thunb. in A549 Human Lung Carcinoma Cells

어성초 메탄올 추출물에 의한 A549 인체 폐암세포 사멸유도에 관한 연구

  • Hong, Su-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control, Dongeui University Graduate School, (BK21 program)) ;
  • Park, Cheol (Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control, Dongeui University Graduate School, (BK21 program)) ;
  • Hong, Sang-Hoon (Department of Internal Medicine, Dongeui University College of Oriental Medicine and Department of Biomaterial Control, dongeui University Graduate School) ;
  • Choi, Byung-Tae (Department of Anatomy, Dongeui University College of Oriental Medicine and Department of Biomaterial Control, dongeui University Graduate School) ;
  • Lee, Yong-Tae (Department of Physiology, Dongeui University College of Oriental Medicine and Department of Biomaterial Control, dongeui University Graduate School)
  • 홍수현 (동의대학교 한의과대학 생화학교실 및 대학원 바이오물질제어학과) ;
  • 박철 (동의대학교 한의과대학 생화학교실 및 대학원 바이오물질제어학과) ;
  • 홍상훈 (동의대학교 한의과대학 내과학교실) ;
  • 최병태 (동의대학교 한의과대학 해부학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실)
  • Published : 2006.12.25
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Abstract

Houttuynia cordata Thunb, well known as 'E-Sung-Cho' in Korea, is traditional medicinal plant generally used in Oriental medicine therapy. We previously reported that the water extract of H. cordata inhibited cell proliferation and induced apoptosis in human breast carcinoma cells. In the present study, we investigated the biochemical mechanisms of anti-proliferative effects by the methanol extract of H. cordata (MEHC) in human lung carcinoma A549 cells. It was found that MEHC could inhibit the cell growth in a dose-dependent manner, which was associated with morphological change and apoptotic cell death as determined by formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase cells. Apoptosis of A549 cells by MEHC was also connected with a down-regulation of anti-apoptotic Bcl-2 and inhibitor of apoptosis proteins (IAPs) expression. MEHC treatment induced the proteolytic activation of caspase-3, caspase-8 and caspase-9, and a concomitant inhibition of poly(ADP-ribose) polymerase (PARP), ${\beta}$-catenin and phospholipase (PLC)-${\gamma}$1 protein expression. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.

Keywords

References

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