Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-apoptotic Effect of Bojungbangam-tang Ethanol Extract on Cisplatin-Induced Apoptosis in Rat Mesangial Cells

  • Kim, Nam-Su (Department of Oriental Regabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon, Young-Dal (Department of Oriental Regabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Shin, Byung-Cheul (Department of Oriental Regabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Song, Yung-Sun (Department of Oriental Regabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • Published : 2006.12.25
Download PDF
⟨ Previous Next ⟩

Abstract

Cisplatin is a anti-neoplastic agent which is commonly used for the treatment of solid tumor. Cisplatin activates multiple signal transduction pathways involved in the stress-induced apoptosis in a variety of cell types. Cytotoxicity of cisplatin was detected in rat mesangial cells and the value of $IC_{50}$ is about 20 ${\mu}M$. The treatment of cisplatin to rat mesangial cells showed the apoptotic bodies and DNA fragmentation. The activation of caspase-3, -8, and -9 and proteolytic cleavage of PARP were observed in the rat mesangial cells treated time-dependently with cisplatin. The activation of ERK, p38 and JNK was also observed in the apoptosis induced by cisplatin in rat mesangial cells. The ethanol extract of Bojungbangam-tang (EBJT), a new hergal prescription composed of nine crude drugs, inhibited cisplatin-induced apoptosis in rat mesangial cells. EBJT reduced sub-G1 peak (apoptotic peak) in cisplatin-treated rat mesangial cells. The cisplatin-induced ERK and JNK activation in rat mesangial cells were blocked by EBJT, but EBJT had no effect on p38 activation. Taken together, these results con suggest that EBJT prevents cisplatin-induced apoptotic cell death in rat mesangial cells through inhibition of ERK and JNK activation.

Keywords

References

  1. Timmer-Bosscha, H., Mulder, N.H. and de Vries, E.G. Modulation of cis-diamminedichloroplatinum(II) resistance: a review. Br. J. Cancer. 66:227-238, 1992 https://doi.org/10.1038/bjc.1992.249
  2. Goldstein, R.S. and Mayor, G.H. Minireview. The nephrotoxicity of cisplatin. Life Sci. 32:685-690, 1983 https://doi.org/10.1016/0024-3205(83)90299-0
  3. Safirstein, R., Winston, J., Goldstein, M., Moel, D., Dikman, S. and Guttenplan, J. Cisplatin nephrotoxicity. Am. J. Kidney Dis. 8:356-367, 1986 https://doi.org/10.1016/S0272-6386(86)80111-1
  4. Eastman, A. The formation, isolation and characterization of DNA adducts produced by anticancer platinum complexes. Pharmacol. Ther. 34:155-166, 1987 https://doi.org/10.1016/0163-7258(87)90009-X
  5. Siddik, Z.H. Cisplatin: mode of cytotoxic action and molecular basis of resistance. Oncogene 22:7265-7279, 2003 https://doi.org/10.1038/sj.onc.1206933
  6. Cross, T.G., Scheel-Toellner, D., Henriquez, N.V., Deacon, E., Salmon, M. and Lord, J.M. Serine/threonine protein kinases and apoptosis. Exp. Cell Res. 256:34-41, 2000 https://doi.org/10.1006/excr.2000.4836
  7. Pearson, G., Robinson, F., Beers Gibson, T., Xu, B.E., Karandikar, M., Berman, K. and Cobb, M.H. Mitogen- activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 22:153-183, 2001 https://doi.org/10.1210/er.22.2.153
  8. Cobb, M.H. MAP kinase pathways. Prog. Biophys. Mol. Biol. 71:479-500, 1999 https://doi.org/10.1016/S0079-6107(98)00056-X
  9. Xia, Z., Dickens, M., Raingeaud, J., Davis, R.J. and Greenberg, M.E. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 270:1326-1331, 1995 https://doi.org/10.1126/science.270.5240.1326
  10. Davis, R.J. Signal transduction by the JNK group of MAP kinases. Cell 103:239-252, 2000 https://doi.org/10.1016/S0092-8674(00)00116-1
  11. Lee, S.J., Saiki, I., Hayakawa, Y., Nunome, S., Yamada, H., Kim, S.H. Antimetastatic and immunomodulating properties of a new herbal prescription, Bojung-bangam-tang. Int. Immunopharmacol. 3:147-157, 2000 https://doi.org/10.1016/S1567-5769(02)00091-7
  12. Hengartner, M.O. The biochemistry of apoptosis. Nature 407:770-776, 2000 https://doi.org/10.1038/35037710
  13. Ito, Y., Pandey, P., Sporn, M.B., Datta, R., Kharbanda, S. and Kufe, D. The novel triterpenoid CDDO induces apoptosis and differentiation of human osteosarcoma cells by a caspase-8 dependent mechanism. Mol. Pharmacol. 59:1094-1099, 2001 https://doi.org/10.1124/mol.59.5.1094
  14. Ito, Y., Pandey, P., Place, A., Sporn, M.B., Gribble, G.W., Honda, T., Kharbanda, S. and Kufe D. The novel triterpenoid 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid induces apoptosis of human myeloid leukemia cells by a caspase-8-dependent mechanism. Cell Growth. Differ. 11:261-267, 2000
  15. Guyton, K,Z,, Liu, Y., Gorospe, M., Xu, Q. and Holbrook, N.J. Activation of mitogen-activated protein kinase by H2O2. Role in cell survival following oxidant injury. J. Biol. Chem. 271:4138-4142, 1996 https://doi.org/10.1074/jbc.271.8.4138
  16. Wang, X., Martindale, J.L., Liu, Y. and Holbrook, N.J. The cellular response to oxidative stress: influences of mitogen-activated protein kinase signalling pathways on cell survival. Biochem. J. 333:291-300, 1998 https://doi.org/10.1042/bj3330291
  17. DeHaan, R.D., Yazlovitskaya, E.M., Persons, D.L. Regulation of p53 target gene expression by cisplatin-induced extracellular signal-regulated kinase. Cancer Chemother. Pharmacol. 48:383-388, 2001 https://doi.org/10.1007/s002800100318
  18. Hayakawa, J., Ohmichi, M., Kurachi, H., Ikegami, H., Kimura, A., Matsuoka, T., Jikihara, H. and Mercola, D., Murata, Y. Inhibition of extracellular signal-regulated protein kinase or c-Jun N-terminal protein kinase cascade, differentially activated by cisplatin, sensitizes human ovarian cancer cell line. J. Biol. Chem. 274:31648-31654, 1999 https://doi.org/10.1074/jbc.274.44.31648
  19. Persons, D.L., Yazlovitskaya, E.M. and Pelling, J.C. Effect of extracellular signal-regulated kinase on p53 accumulation in response to cisplatin. J. Biol. Chem. 275:35778-357785, 2000 https://doi.org/10.1074/jbc.M004267200
  20. Nowak, G. Protein kinase C-alpha and ERK1/2 mediate mitochondrial dysfunction, decreases in active Na+ transport, and cisplatin-induced apoptosis in renal cells. J. Biol. Chem. 277:43377-43388, 2002 https://doi.org/10.1074/jbc.M206373200
  21. Sanchez-Perez, I., Murguia, J.R. and Perona, R. Cisplatin induces a persistent activation of JNK that is related to cell death. Oncogene 16:533-540, 1998 https://doi.org/10.1038/sj.onc.1201578
  22. Kim, Y.K., Kim, H.J., Kwon, C.H., Kim, J.H., Woo, J.S., Jung, J.S. and Kim, J.M. Role of ERK activation in cisplatin-induced apoptosis in OK renal epithelial cells. J. Appl. Toxicol. 25:374-382, 2005 https://doi.org/10.1002/jat.1081
  23. Wang, X., Martindale, J.L. and Holbrook, N.J. Requirement for ERK activation in cisplatin-induced apoptosis. J .Biol. Chem. 275:39435-39443, 2000 https://doi.org/10.1074/jbc.M004583200
  24. Fan, M. and Chambers, T.C. Role of mitogen-activated protein kinases in the response of tumor cells to chemotherapy. Drug Resist. Updat. 4:253-267, 2001 https://doi.org/10.1054/drup.2001.0214
  25. Zanke, B.W., Boudreau, K., Rubie, E., Winnett, E., Tibbles, L.A., Zon, L., Kyriakis, J., Liu, F.F. and Woodgett, J.R. The stress-activated protein kinase pathway mediates cell death following injury induced by cis-platinum, UV irradiation or heat. Curr. Biol. 6:606-613, 1996 https://doi.org/10.1016/S0960-9822(02)00547-X
  26. Sanchez-Perez, I. and Perona, R. Lack of c-Jun activity increases survival to cisplatin. FEBS Lett. 453:151-158 https://doi.org/10.1016/S0014-5793(99)00690-0
  27. Mishima, K., Baba, A., Matsuo, M., Itoh, Y. and Oishi, R. Protective effect of cyclic AMP against cisplatin-induced nephrotoxicity. Free Radic. Biol. Med. 40:1564-1577, 2006 https://doi.org/10.1016/j.freeradbiomed.2005.12.025
  28. Brozovic, A., Fritz, G., Christmann, M., Zisowsky, J., Jaehde, U., Osmak, M. and Kaina, B. Long-term activation of SAPK/JNK, p38 kinase and fas-L expression by cisplatin is attenuated in human carcinoma cells that acquired drug resistance. Int. J. Cancer 112:974-985, 2004 https://doi.org/10.1002/ijc.20522
  29. Guadagno, T,M, and Ferrell, J.E. Jr. Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts. Science 282:1312-1315, 1998 https://doi.org/10.1126/science.282.5392.1312
  30. Wright, J.H., Munar, E., Jameson, D.R., Andreassen, P.R., Margolis, R.L., Seger, R. and Krebs, E.G. Mitogen-activated protein kinase kinase activity is required for the G(2)/M transition of the cell cycle in mammalian fibroblasts. Proc. Natl. Acad. Sci. USA. 96:11335-11340, 1999
  31. Bulavin, D.V., Higashimoto, Y., Popoff, I.J., Gaarde, W.A., Basrur, V., Potapova, O., Appella, E. and Fornace, A.J. Jr. Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase. Nature 411:102-107, 2001 https://doi.org/10.1038/35075107
  32. Wang, X., McGowan, C.H., Zhao, M., He, L., Downey, J.S., Fearns, C., Wang, Y., Huang, S. and Han, J. Involvement of the MKK6-p38gamma cascade in gamma-radiation- induced cell cycle arrest. Mol. Cell Biol. 20:4543-4552, 2000 https://doi.org/10.1128/MCB.20.13.4543-4552.2000