Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of 4 Weeks Endurance Exercise on Expression of Extracellular Signal-Regulated Kinases and c-Jun N-terminal Kinase in Rat Back Skin Hair Follicle

4주간 지구성 운동이 흰쥐의 Back Skin Hair Follicle에서 ERK 및 JNK의 활성화에 미치는 영향

  • Kim, Mo-Kyung (Graduate School of Physical Education, Kunsan National University) ;
  • Park, Han-Su (Graduate School of Physical Education, Kunsan National University) ;
  • Jo, Sung-Cho (Graduate School of Physical Education, Kunsan National University) ;
  • Chae, Jeong-Ryong (Graduate School of Physical Education, Kunsan National University) ;
  • Kim, Mo-Young (Graduate School of Public Administration, Jeonju University) ;
  • Shin, Byung-Cheul (College of Oriental Medicine, Wonkwang University)
  • 김모경 (군산대학교 자연과학대학원) ;
  • 박한수 (군산대학교 자연과학대학원) ;
  • 조성초 (군산대학교 자연과학대학원) ;
  • 채정룡 (군산대학교 자연과학대학원) ;
  • 김모영 (전주대학교 행정대학원) ;
  • 신병철 (원광대학교 한의과대학)
  • Published : 2006.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

The effect of a chronic programme of either low- or moderate-to-high-intensity treadmill running on the activation of the Extracellular-signal regulated protein kinase (ERK1/2), Phosphorylated ERK 1/2(pERK1/2) and the Phosphorylated c-Jun N-terminal kinase(pJNK) pathways was determined in rat Back skin Hair follicle. Sprague-Dawley rats were assigned to one of three groups: (i) sedentary group(NE; n=10); (ii) low-intensity exercise group (Bm/min; LIE; n=10); and (iii) moderate-high-intensity exercise group(28m1min; HIE; n=10). The training regimens were planned so that animals covered the same distance and had similar utilization for both LIE and HIE exercise sessions. The report runs as follows; A single bout of LIE or HIE following 4 weeks of exercise led to a twofold increase in the phosphorylation of ERK2, pERK2 and a threefold increase in pJNKl, pERKl. ERKI phosphorylation in LIE Back skin sampled and pJNK2 in HIE Back skin sampled 48h after the last exercise bout was similar to sedentary values, while pJNK2 phosphorylation in LIE Back skin sampled was 70-80% lower than sedentary. 48h after the last exercise bout of LIE or HIE increased ERK2, pERKl and pJNKl expression, with the magnitude of this increase being independent of prior exercise intensity or duration. PERK1/2, pJNKl expression was increased Three- to fourfold in Back skin Hair follicle sampled 48h after the last exercise bout irrespective of the prior exercise programme, but ERKI expression in HIE Back skin sampled was approximately 90% lower than sedentary values. In conclusion, exercise-training of different jntensities/durations results in selective postexercise activation of intracellular signal pathways, which may be one mechanism regulating specific adaptations induced by diverse training programmes.

Keywords

References

  1. Seger, R., Krebs, E.G. The MAPK signaling cascade. FASEB J 9: 726, 1995 https://doi.org/10.1096/fasebj.9.9.7601337
  2. Petrazzuloi, M., Goldsmith, L.A. Molecular mechanisms of cell signaling, In: Fitzpatrick, T.B., Eisen, A.Z., Wolff, K., Freedberg, I.M., et al. Dermatology in General Medicine. 5th ed. New York: McGraw-Hill, pp 114-131, 1999
  3. Cano, E., Mahadevan, L.C. Parallel signal processing among mammaliam MARKs. Trends Biochem Sci. 20: 117-122, 1995 https://doi.org/10.1016/S0968-0004(00)88978-1
  4. Assefa, Z., Garmyn, M., Bouillon, R., Merlevede, W., et al Differential stimulation of ERK and JNK activities by ultraviolet B irradiation and epidermal growth factor in human keratinocytes. J Invest Dermatol. 108: 886-891, 1997 https://doi.org/10.1111/1523-1747.ep12292595
  5. Albanell, J., Codony-Servat, J., Rojo, F., Campo, J.M., Sauleda, S., Anido, J., et al. Activated extracellular signal-regulated kinases: Association with epidermal growth factor receptor/transforming growth factor $\alpha$ expression in head and neck squamous carcinoma and inhibition by anti-epidermal growth factor receptor treatments. Cancer Res X 61: 6500-6510, 1999
  6. Geilen, C.C., Wieprecht, M., Prfanos, C.E. The mitogen- activated protein kinases systerm (MAP kinase cascade):its role in skin signal transduction. A review. J Dermatol Sci. 12: 255-262, 1996 https://doi.org/10.1016/0923-1811(95)00481-5
  7. Chen, R.H., Sarnecki, C., Blenis, J. Nuclear localization and regulation of erk-and-rsk-encoded protein kinases. Mol Cell Biol. 12: 915-27, 1992 https://doi.org/10.1128/MCB.12.3.915
  8. Gille, H., Kortenjann, M., Thomae, O., Moomaw, C., Slaughter, C., Cobb, M.H., Shaw, P.E. ERK phosphoryation potentiate Elk-1-mediated ternary complex formation and transactivation. EMBO J, 14: 951-962, 1995
  9. Zinck, R., Cahill, M.A., Kracht, M., Sachsenmaier, C., Hipskind, R.A., Nordheim, A. Protein synthesis inhibitors reveal differential regulation of mitogen-activated protein kinase stress-activated protein kinase pasthways that converge on Elk-1. Mol Cell Biol. 15: 4930-4939, 1995 https://doi.org/10.1128/MCB.15.9.4930
  10. Sgambato, V., Vanhoutte, P., Pages, C., Rogaed, M., Hipskind, R., Besson, M.J., Caboche, J. In vivo expression and regulation of Elk-1, a target of the extracellular-regulated kinase signaling pathway, in the adult rat brain. J Neurosci. 18: 214-226, 1998 https://doi.org/10.1523/JNEUROSCI.18-01-00214.1998
  11. Vanhoutte, P., Barnier, J.V., Guibert, B., Pages, C., Besson, M.J., Hipskind, R.A. Glutamate induces phosphorylation of Elk-1 and CREB, along with d-fos activation, via an extracellular signal-regulated kinase-dependent pathway in brain slices, Mol Cell Biol, 19: 136-146, 1999 https://doi.org/10.1128/MCB.19.1.136
  12. Ruiter, G.A., Zerp, S.F., Bartelink, H., van Blitterswijk, W.J., Verheij, M. Alkyl-lysopholipids activate the SAPK/JNK pathway and enhance radiation-induced apoptosis. Cancer Res;59,10: 2457-2463, 1999
  13. Schwenger, P., Alpert, C., Skolnik, E.Y., Vilcek, J. Cell-type-specific activation of c-Jun N-terminal kinase by salicylates, J Cell Physiol 179: 109-114, 1999 https://doi.org/10.1002/(SICI)1097-4652(199904)179:1<109::AID-JCP13>3.0.CO;2-W
  14. Burrerfield, L., Storey, B., Mass, L., Heasley, L.E. c-Jun N-terminal kinase regulation lf small cell oung cancer cells to ultraviolet radiation. J Biol Chem 272, 10110-10116, 1997 https://doi.org/10.1074/jbc.272.15.10110
  15. Zhang, P., Hogan, E.L., Bhat, N.R. Activation of JNK/SAPK in primary glial cultures: II Differential activation lf kinase isofprms corresponds to their differential expression, Neurochen, Neurochem Res 23: 219-225, 1998 https://doi.org/10.1023/A:1022489127107
  16. 손인범, 이원수. 인체모낭기관배양에서 substance P 가 모발성장에 미치는 영향. 대한피부과학회지 39: 190-198, 2001
  17. Seiniya, H., Mashima, T., Toho, M., Tsuruo, T. c-Jun N-terminal kinase-mediated activation of interleukin-1beta converting enzyme/CED-3-like protease diromg amticancer. J Biol Chem 272: 4631-4636, 1997 https://doi.org/10.1074/jbc.272.7.4631
  18. Xia, Z., Dickens, M., Raingeaud, J., Davis, R., Greenberg, M.E. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 270: 1326-1331, 1995 https://doi.org/10.1126/science.270.5240.1326
  19. Ortiz, M.A., Lopez-hernandez, F.J., Bayon, Y., Pfahl, M., Piedrafita, F.J. Retinoid-related molecules induce cytochrome c release and apoptosis through activation of c-Jun N-terminal kinase/p38 mitogen-activated protein kinases. Cancer Res 61: 8504-8512, 2001
  20. Winkelmann, R.K. Cutaneous sensory nerves. Semin Dermatopathol pp 236-268, 1988
  21. Lee, W.S., Sohn, I.B. Substance P prolongs human hair growth in vitro. J Dermatol Sci 33: 137-138, 2003 https://doi.org/10.1016/j.jdermsci.2003.08.003
  22. Mitsui, S., Ohuchi, A., Hotta, M., Tsuboi, R., Ogawa, H. Genes for a range of growth factors and cyclin-dependent kinase inhibitors are expressed by isolated human hair follicles. Br K Dermatol 137: 693-698, 1997 https://doi.org/10.1111/j.1365-2133.1997.tb01103.x
  23. Soma, T., Ogo, M., Suzuri, K., Takahashi, T., Hibino, T. Analysis lf apoptotic cell death in human hair flooicles in vivo and in vitro. K invest Dermatol ;111:948-954, 1998 https://doi.org/10.1046/j.1523-1747.1998.00408.x
  24. Werner, S., Smola, H., Liao, X., Longaker, M.T., Krieg, T., Hofschneider, P.H., Williams, L.T. The function lf KGF in morphogenesis of epithelium and reepithelialization of wounds. Science 266: 819-822, 1994 https://doi.org/10.1126/science.7973639
  25. Itami, S., Kurata, S., Takayasu, S. Androgen induction of follicular epithelial cell growth is mediated via insulin-like growth factor-1 from dermal papilla cells. Biochem Biophys Res Commun 212: 988-994, 1995 https://doi.org/10.1006/bbrc.1995.2067
  26. Lachgar, S., Charveron, M., Sarraute, K., Gall, Y., Bonafe, J.L. Antiandrogens and estrogens: modulators lf VEGF expression in cultured hair dermal papilla cells. Exp Dermatol 8: 336-338, 1999
  27. Hoffmann, R., Happle, R. Does interleukin-1 induce hair loss? Dermatology 191: 273-275, 1995 https://doi.org/10.1159/000246567
  28. Hamada, K., Hirotsu, S., Uchiwa, H., Yamazaki, S., Suzuki, K. Proinflammatory cytokine interleukin-1a is downregulated during anagen phase of hair cycle in vivo. K Dermatol Sci 33: 195-198, 2003 https://doi.org/10.1016/j.jdermsci.2003.08.001
  29. Hoffmann, R., Eicheler, W., Wenzel, E., Happle, R. Interleukin- 1$\beta$-induced inhibition of hair growth in vitro is mediated by cyclic AMP. J Invest Dermatol 108: 40-42, 1997 https://doi.org/10.1111/1523-1747.ep12285625
  30. Takahashi, H., Honma, M., Ishida-Yamamoto, A., Namikawa, K., et al. Expression of human cystatin A by keratinocytes is positively regulated via the Ras/MEKK1/MKK7/JNK signal transduction pathway but negatively regulated via the Ras/Raf-1/MEK1/ERK pathway. J Biol Chem. 276(39):36632-36638, 2001 https://doi.org/10.1074/jbc.M102021200
  31. Takagashi, H., Ibe, M., Nakamura, S., Ishida-Yamamoto, A., et al. Extracellular regulated kinase and c-Jun N-terminal kinase are activated in psoriatic involved epidermis. J Dermatol sci. 30: 94-99, 2002 https://doi.org/10.1016/S0923-1811(02)00064-6
  32. Elder, J.T., Fisher, G.J., Lindquist, P.B., Bennett, G.L., et al. Overexpression of transforming growth factor $\alpha$ in pworiatic epidermis. Science 243: 811-814, 1989 https://doi.org/10.1126/science.2916128
  33. Bost, F., Mckay, R., Bost, M., Potapova, O., et al. The Jun Kinase 2 isoform is preferentially required for epidermal growth factor-induced transformation of human A549 lung carcinoma cells. Mol Cell Biol 19: 1938-1949, 1999 https://doi.org/10.1128/MCB.19.3.1938
  34. Derijard, B., Hibi, M., Wu, I.H., Barrett, T., Su, B., Deng, T., et al. JNK1: A protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76: 1025-1037, 1994 https://doi.org/10.1016/0092-8674(94)90380-8
  35. Sayama, K., Hanakawa, Y., Shirakata, Y., Yamasaki, K., et al. Apoptosis signal-regulating kinase 1 (ASK1) es an intracellular inducer of keratinocyte differentiation. J Biol Chem. 276: 999-1004, 2001 https://doi.org/10.1074/jbc.M003425200
  36. Santos, M., Perez, P., Segrelles, C., Ruoz, S., et al. Impaired NF-kappa B activation and incerased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis. J Biol Chem 278: 13422-13430, 2003 https://doi.org/10.1074/jbc.M208170200
  37. Chen, Y.R., Meyer, C.F., Tan, T.H. Persistent activation of c-Jun N-terminal kinase 1(JNK1) in gamma radition-induced apoptosis. J Biol Chem. 271: 631-634, 1996 https://doi.org/10.1074/jbc.271.2.631
  38. Fuchs, S.Y., Adler, V., Pincus, M.R., Ronai, Z. MAKK1/JNK signaling stabilizes and activates p53. Proc. Natl. Acad. Sci. USA 95: 10541-10546, 1998