동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제20권5호
  • /
  • Pages.1271-1274
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  • 2006
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

한약재 12종의 열수추출물이 ${\alpha}$-melanocyte stimulating hormone에 의해 유도된 B16F10 흑색종 세포의 멜라닌형성에 미치는 영향

Effects of Aqueous Extracts from Twelve Herbs on ${\alpha}$-melanocyte Stimulating Hormone-induced Melanogenesis in B16F10 Mouse Melanoma Cell

  • 이수진 (동의대학교 한의과대학 해부학교실) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실) ;
  • 최병태 (동의대학교 한의과대학 해부학교실)
  • Lee, Soo-jin (Departments of Anatomy, College of Oriental Medicine, Dong-Eui University) ;
  • Choi, Yung-Hyun (Departments of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, Yong-Tae (Departments of Physiology, College of Oriental Medicine, Dong-Eui University) ;
  • Choi, Byung-Tae (Departments of Anatomy, College of Oriental Medicine, Dong-Eui University)
  • 발행 : 2006.10.25
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초록

We investigated the effects of aqueous extracts from twelve medical herbs on ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH)-induced melanogenesis in B16F10 mouse melanoma cell. The cells were incubated with ${\alpha}$-MSH and aqueous extracts 5 days and 2 days and then analysed melanin amount and tyrosinase activities, respectively. Nine aqueous extract of herbs examined at 1 mg/m${\ell}$ level decreased melanin synthesis in B16F10 cell, especially in Agastache rugosa, Leonurus siviricus and Murus bombycis. The significant decrease of released extracellular melanin were also observed in treated cells with aqueous extract from Leonurus siviricus, Murus bombycis and Ledebouriella seseioides. The ${\alpha}$-MSH-induced activation of tyrosinase was inhibited in cells treated with aqueous extract from Cuscutae semen, Angelica tenuissima and Agastache rugosa. These results suggest that herbs inhibiting melanogenesis through tyrosinase activity may apply to develop whitening drugs and cosmetics.

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참고문헌

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