DOI QR코드

DOI QR Code

Mapping, Tissue Distribution and Polymorphism Study of the Porcine SOCS2 and SOCS3 Genes

  • Li, X.Y. (School of Animal Science and Technology, Huazhong Agricultural University) ;
  • Liu, B. (School of Animal Science and Technology, Huazhong Agricultural University) ;
  • Fan, B. (School of Animal Science and Technology, Huazhong Agricultural University) ;
  • Yu, M. (School of Animal Science and Technology, Huazhong Agricultural University) ;
  • Zhu, M.J. (School of Animal Science and Technology, Huazhong Agricultural University) ;
  • Xiong, T.A. (School of Animal Science and Technology, Huazhong Agricultural University) ;
  • Li, K. (School of Animal Science and Technology, Huazhong Agricultural University)
  • Received : 2005.02.04
  • Accepted : 2005.07.22
  • Published : 2006.02.01

Abstract

Using the somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel, porcine SOCS2 gene was mapped at SSC5 (1/2) q21-q24 and closely linked with SW1383 marker (47 cR in distance), while SOCS3 gene was assigned to SSC12p11-(2/3p13) and closely linked with SW2490 (43 cR). The reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect the expression of these two genes in the different tissues and the results showed that both SOCS2 and SOCS3 genes were widely expressed in tissues investigated (heart, liver, spleen, lung, kidney skeletal muscle, fat and brain), although some tissues showed lower gene expression. Moreover, SOCS2 and SOCS3 genes had different expression levels at different stages, in different tissues and in different breeds. A G/A substitution, which can be recognized by restriction enzyme of Cfr421, was observed in 5' untranslated region (5'-UTR) of SOCS2 gene. The allele frequencies was investigated by PCR-restriction fragment length polymorphism (PCR-RFLP) method and it showed that the allele frequency among Dahuabai, Erhualian, Yushan, Qingping, Large white and Landrace tested were different. Association analysis in a cross experimental populations revealed no significant association between the SOCS2 gene polymorphism and the economic traits investigated. The full-length coding regions (CDs) of porcine SOCS3 gene was obtained by RT-PCR.

Keywords

References

  1. Chevalet, C., J. Gouzy and M. San Cristobal-Gaudy. 1997. Regional assignment of genetic markers using a somatic cell hybrid panel: a WWW interactive program available for the pig genome. Computer Application Bioscience 1:69-73
  2. Chowdhary, B. P., P. D. Thomsen, I. Harbitz, M. Landset and I. Gustavsson. 1994. Precise localization of the genes for glucose phosphate isomerase (GPI), calcium release channel (CRC), hormone-sensitive lipase (LIPE), and growth hormone (GH) in pigs, using nonradioactive in situ hybridization. Cytogenet. Cell Genet. 67:211-214 https://doi.org/10.1159/000133825
  3. Dey, B. R., S. L. Spence, P. Nissley and R. W. Furlanetto. 1998. Interaction of human suppressor of cytokine signaling (SOCS)-2 with the insulin-like growth factor-I receptor. J. Biol. Chem. 273:24095-24101 https://doi.org/10.1074/jbc.273.37.24095
  4. Greenhalgh, C. J., P. Bertolino, S. L. Asa, D. Metcalf, J. E. Corbin, T. E. Adams, H. W. Davey, N. A. Nicola, D. J. Hilton and W. S. Alexander. 2002. Growth enhancement in suppressor of cytokine signaling 2 (SOCS-2)-deficient mice is dependent on signal transducer and activator of transcription 5b (STAT5b). Mol. Endocrinol. 16:1394-1406 https://doi.org/10.1210/me.16.6.1394
  5. Greenhalgh, C. J., D. Metcalf, A. L. Thaus, J. E. Corbin, R. Uren, P. O. Morgan, L. J. Favri, J. G. Zhang, H. M. Martin, T. A. Willson, N. Billestrup, N. A. Micola, M. Baca, W. S. Alexander and D. J. Hilton. 2002. Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling. J. Biol. Chem. 277:40181-40184 https://doi.org/10.1074/jbc.C200450200
  6. Hilton, D. J., R. T.Richardson, W. S.Alexander, E. M. Viney, T. A. Willson, N. S. Sprigg, R. Starr, S. E. Nicholson and N. A. Nicola. 1998. Twenty proteins containing a C-terminal SOCS box form five structural classes. Proc. Natl. Acad. Sci. USA. 95:114-119
  7. Janzen, M. A., D. L.Kuhlers, S. B. Jungst and C. F. Louis. 2000. ARPP-16 mRNA is up-regulated in the longissimus muscle of pigs possessing an elevated growth rate. J. Anim. Sci. 78:1475-1484
  8. Lehmann, U., J. Schmitz, M. Weissenbach, R. M. Sobota, M. Hortner, K. Friederichs, I. Behrmann, W. Tsiaris, A. Sasaki, J. Schneider-Mergener, A.Yoshimura, B. G. Neel, P. C. Heinrich and F. Schaper. 2003. SHP2 and SOCS3 contribute to Tyr-759- dependent attenuation of interleukin-6 signaling through gp130. J. Biol. Chem. 278:661-671 https://doi.org/10.1074/jbc.M210552200
  9. Masuhara, M., H. Sakamoto, A. Matsumoto, R. Suzuki, H. Yasukawa, K. Mitsui, T. Wakioka, S. Tanimura, A. Sasaki, H. Misawa, M. Yokouchi, M. Ohtsubo and A. Yoshimura. 1997. Cloning and characterization of novel CIS family genes. Biochem. Biophys. Res. Commun. 239:439-446 https://doi.org/10.1006/bbrc.1997.7484
  10. A. Nicola, J. Douglas, D. J. Hilton and W. S. Alexander. 2000. Gigantism in mice lacking suppressor of cytokine signalling-2. Nature 405:1069-1073 https://doi.org/10.1038/35016611
  11. Milan, D., R. Hawken, C. Cabau, S. Leroux, C. Genet, Y. Lahbib, G. Tosser, A. Robic, F. Hatey, L. Alexander, C. Beattie, L. Schook, M. Yerle and J. Gellin. 2000. IMpRH server: an RH mapping server available on the web. Bioinformatics 16:558-559 https://doi.org/10.1093/bioinformatics/16.6.558
  12. Pan, P. W., S. H. Zhao, M. Yu, B. Liu, T. A. Xiong and K. Li. 2003. Identification of differentially expressed genes in the longissimus dorsi muscle tissue between duroc and erhualian pigs by mRNA differential display. Asian-Aust. J. Anim. Sci. 16:1066-1070 https://doi.org/10.5713/ajas.2003.1066
  13. Roberts, A. W., L. Robb, S. Rakar, L. Hartley, L. Cluse, N. A. Nicola, D. Metcalf, D. J. Hilton and W. S. Alexander. 2001. Placental defects and embryonic lethality in mice lacking suppressor of cytokine signaling 3.Proc. Natl. Acad. Sci. USA. 98:9324-9329
  14. Starr, R., T. A. Willson, E. M. Viney, L. J. Murray, J. R. Rayner, B. J. Jenkins, T. J. Gonda, W. S. Alexander, D. Metcalf, N. A. Nicola and D. J. Hilton. 1997. A family of cytokine-inducible inhibitors of signalling. Nature 387:917-921 https://doi.org/10.1038/43206
  15. Tollet-Egnell, P., Flores-Morales, A. Stavreus-Evers, L. Sahlin and G. Norstedt. 1999. Growth Hormone Regulation of SOCS-2, SOCS-3, and CIS Messenger Ribonucleic Acid Expression in the Rat. Endocrinol. 140:3693-3704 https://doi.org/10.1210/en.140.8.3693
  16. Yandava, C. N., A. Pillari and J. M. Drazen. 1999. Radiation hybrid and cytogenetic mapping of SOCS1 and SOCS2 to chromosomes 16p13 and 12q, respectively. Genomics 61:108-11 https://doi.org/10.1006/geno.1999.5937
  17. Yasukawa, H., M. Ohishi, H. Mori, M. Murakami, T. Chinen, D. Aki, T. Hanada, K. Takeda, S. Akira, M. Hoshijima, T. Hirano, K. R. Chien and A. Yoshimura. 2003. IL-6 induces an antiinflammatory response in the absence of SOCS3 in macrophages. Nat. Immunol. 4:551-556 https://doi.org/10.1038/ni938
  18. Yerle, M., G. Echard, A. Robic, A. Mairal, C. Dubut-Fontana, J. Riquet, P. Pinton, D. Milan, Y. Lahbib-Mansais and J. Gellin. 1996. A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet. Cell Genet. 73:194-202 https://doi.org/10.1159/000134338
  19. Yerle, M., P. Pinton, A. Robic, A. Alfonso, Y. Palvadeau, C. Delcros, R. Hawken, L. Alexander, L. B. Beattie, D. Milan and J. Gellin. 1998. Construction of a wholegenome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet. Cell Genet. 82:182-188 https://doi.org/10.1159/000015095
  20. Zeng, Y. Q., G. L. Wang, C. F. Wang, S. D. Wei, Y. Wu, L. Y. Wang, H. Wang and H. L. Yang. 2005. Genetic variation of H-FABP gene and association with intramuscular fat content in Laiwu Black and four western pig breeds. Asian-Aust. J. Anim. Sci. 18:13-16 https://doi.org/10.5713/ajas.2005.13
  21. Zhao, S. H. and C. K. Tuggle. 2005. Mapping of the porcine suppressor of cytokine signaling 3 (SOCS3) gene to chromosome 12 by using somatic cell and radiation hybrid panels. Anim. Genet. (in press)

Cited by

  1. Expression Characterization, Polymorphism and Chromosomal Location of the Porcine Calsarcin-3 Gene vol.20, pp.9, 2006, https://doi.org/10.5713/ajas.2007.1349