Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Synchronizing the Rate of Dietary Energy and Nitrogen Release on Ruminal Fermentation, Microbial Protein Synthesis, Blood Urea Nitrogen and Nutrient Digestibility in Beef Cattle

  • Chumpawadee, Songsak (Department of Agricultural Technology, Faculty of Technology, Mahasara Kham University) ;
  • Sommart, K. (Department of Animal Science, Faculty of Agriculture, Khon Kaen University) ;
  • Vongpralub, T. (Department of Animal Science, Faculty of Agriculture, Khon Kaen University) ;
  • Pattarajinda, V. (Department of Animal Science, Faculty of Agriculture, Khon Kaen University)
  • Received : 2004.12.28
  • Accepted : 2005.06.25
  • Published : 2006.02.01
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Abstract

The objective of this research was to determine the effects of synchronizing the rate of dietary energy and nitrogen release on: ruminal fermentation, microbial protein synthesis, blood urea nitrogen, and nutrient digestibility in beef cattle. Four, two-and-a-half year old Brahman-Thai native crossbred steers were selected for the project. Each steer was fitted with a rumen cannula and proximal duodenal cannula. The steers were then randomly assigned in a $4{\times}4$ Latin square design to receive four dietary treatments. Prior to formulation of the dietary treatments, feed ingredients were analyzed for chemical composition and a nylon bag technique was used to analyze the treatments various ingredients for degradability. The treatments were organized in four levels of a synchrony index (0.39, 0.50, 0.62 and 0.74). The results showed that dry matter digestibility trend to be increased (p<0.06), organic matter and acid detergent fiber digestibility increased linearly (p<0.05), while crude protein and neutral detergent fiber digestibility were not significantly different (p>0.05). Higher concentration and fluctuation of ruminal ammonia and blood urea were observed in the animal that received the lower synchrony index diets. As the levels of the synchrony index increased, the concentrations of ruminal ammonia nitrogen and blood urea nitrogen, at the 4 h post feeding, decreased linearly (p<0.05). Total volatile fatty acid and bacteria populations at the 4 h post feeding increased linearly (p<0.05). Microbial protein synthesis trend to be increase (p<0.08). The results of this research indicate that synchronizing the rate of degradation of dietary energy and nitrogen release improves ruminal fermentation, microbial protein synthesis and feed utilization.

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References

  1. AFRC. 1992. Technical Committee on Response to Nutrients No.9. Nutritive Requirements of Ruminant Animal: Protein Nutrition Abstracts and Reviews (Series B). 62:787-818
  2. AOAC. 1990. Official Methods of Analysis Association. 15th edn. Association of Official Analytical Chemist. Arlington, Virginia
  3. Arieli, A., Z. Shabi, I. Bruckental, H. Tagari, Y. Aharoni, S. Zamwell and H. Voet. 1996. Effect of the degradation of organic matter and crude protein on ruminal fermentation in dairy cows. J. Dairy Sci. 79:1774-1780 https://doi.org/10.3168/jds.S0022-0302(96)76545-1
  4. Blummel, M., A., I. Givens, H. P. S. Makkar and K. Becker. 1999. Preliminary studies on the relationship of microbial efficiencies of roughage in vitro and methane production in vivo. Proc. SOC. Nutr. Physiol. 8:76-85
  5. Bremner, J. M. and D. R. Keeney. 1965. Steam distillation methods of determination of ammonia, nitrate and nitril. Anal. Chem. Acta. 32:485-495 https://doi.org/10.1016/S0003-2670(00)88973-4
  6. Briggs, P. K., J. F. Hogan and R. L. Reid. 1957. The effect of volatile fatty acid, lactic acid, and ammonia on rumen pH in sheep. Aust. J. Agric. Res. 8:674-710 https://doi.org/10.1071/AR9570674
  7. Cecava, M. J., N. R. Merchen, L. C. Gay and L. L. Berger. 1990. Composition of ruminal bacterial harvested from steers as influenced by dietary energy-level, feeding frequency, and isolation techniques. J. Dairy Sci. 73:2480-2488 https://doi.org/10.3168/jds.S0022-0302(90)78933-3
  8. Chamberlain, D. G. and J. J. Choung. 1995. The importance of rate of ruminal fermentation of energy sources in diets for dairy cows. In Recent Advances in Animal Nutrition. (Ed. P. C. Garnsworthy and D. J. A. Cole). Nothingham: Nothingham University Press. pp. 3-27
  9. Chanjula, P., M. Wanapat, C. Wachirapakorn and P. Rowlinson. 2004. Effect of synchronizing starch sources and protein (NPN) in the rumen on feed intake, rumen microbial fermentation, nutrient utilization and performance of lactating dairy cows. Asian-Aust. J. Anim Sci. 17(10):1400-1410 https://doi.org/10.5713/ajas.2004.1400
  10. Chen, C. and J. Hsu. 1998. The effect of starch and protein degradation rates, hay sources and feeding frequency on rumen microbial fermentation in continuous culture system. In: Proceedings of National Science Council, Republic of China, Part B Life Science, 22:159-165
  11. Czerkawski, J. W. 1986. An Introduction to Ruminal Studies. Oxford: Pergamon Press (Ed. H. J. Finlayson) 1986. The effect of pH on the growth and metabolism of Streptococcus bovis in continuous culture. J. Appl. Bacteriol. 61:201-208 https://doi.org/10.1111/j.1365-2672.1986.tb04277.x
  12. Galyean, M. 1989. Laboratory Procedure in Animal Nutrition Research. Department of animal and rang sciences. New Mexico State University, USA
  13. Henning, P. H., D. G. Steyn and H. H. Meissner. 1991. The effect of energy and nitrogen supply pattern on rumen bacterial growth in vitro. Anim. Prod. 53:165-175 https://doi.org/10.1017/S0003356100020080
  14. Hoover, W. H. 1986. Chemical factors involved in ruminal fiber digestion. J. Dairy Sci. 69:2755-2766 https://doi.org/10.3168/jds.S0022-0302(86)80724-X
  15. Hoover, W. H. and S. R. Stokes. 1991. Balancing carbohydrates and proteins for optimum rumen microbial yield. J. Dairy Sci. 74:3630-3644 https://doi.org/10.3168/jds.S0022-0302(91)78553-6
  16. Joo, J. W., G. S. Bae, W. K. Min, H. S. Chai, W. J. Maeng, Y. H. Chung and M. B. Chang. 2005. Effect of protein sources on rumen microbial protein synthesis using rumen simulated continuous culture system. Asian-Aust. J. Anim. Sci. 18(3):326-331 https://doi.org/10.5713/ajas.2005.326
  17. Khorasani, G. R., G. Deboer, B. Robinson and J. J. Kennelly. 1994. Influence of dietary protein and starch on production and metabolic responses of dairy cows. J. Dairy Sci. 77:813-284 https://doi.org/10.3168/jds.S0022-0302(94)77016-8
  18. Kim, C. H. 2001. Effect of different protein sources given synchronously or asynchronously in to the rumen of consuming a beef cattle diet high in concentrate on the synthesis of microbial protein. J. Anim Sci. Technol. 43:831-840
  19. Kim, K. H., S. S. Lee and K. J. Kim. 2005. Effect of in traruminal sucrose infusion on volatile fatty acid production and microbial protein synthesis in sheep. Asian-Aust. J. Anim. Sci. 18(3):350-353
  20. Kolver, E., L. D. Muller, G. A. Varga and T. J. Cassidy. 1998. Synchronization of ruminal degradation of supplemental carbohydrate with pasture nitrogen in lactation dairy cows. J. Dairy Sci. 81:2017-2028 https://doi.org/10.3168/jds.S0022-0302(98)75776-5
  21. Nocek, J. E. and J. B. Russell. 1988. Protein and energy as on integrated system. Relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production. J. Dairy Sci. 71:2070-2083 https://doi.org/10.3168/jds.S0022-0302(88)79782-9
  22. Orskov, E. R. and I. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92:499-504 https://doi.org/10.1017/S0021859600063048
  23. SAS. 1996. SAS User's Guide: Statistics, Version 6.12th Edition. SAS Institute Inc. Cary, NC
  24. Siddons, R. C., J. Paradine, D. E. Beever and P. R. Cornell. 1985. Ytterbium acetate as a particulate-phase digesta-flow marker. Br. J. Nutr. 54:509-519 https://doi.org/10.1079/BJN19850136
  25. Shabi, Z., A. Arieli, L. Bruckental, Y. Aharoni, S. Zamwel, A. Bor and H. Tagari. 1998. Effect of the synchronization of the degradation of dietary crude protein and organic matter and feeding frequency on ruminal fermentation and flow of digesta in the abomasum of dietary cows. J. Dairy Sci. 81:1991-2000 https://doi.org/10.3168/jds.S0022-0302(98)75773-X
  26. Sinclair, L. A., P. C. Garnsworthy, J. R. Newbold and P. J. Buttery. 1993. Effect of synchronizing the rate of dietary energy and nitrogen release on rumen fermentation and microbial protein synthesis in sheep. J. Agric. Sci. 120:251-263 https://doi.org/10.1017/S002185960007430X
  27. Sinclair, L. A., P. C. Garnsworthy, J. R. Newbold and P. J. Buttery. 1995. Effects of synchronizing the rate of dietary energy and nitrogen release in diets with a similar carbohydrate composition on rumen fermentation and microbial protein synthesis in sheep. J. Agric. Sci. 124:463-472 https://doi.org/10.1017/S0021859600073421
  28. Sinclair, K. D., L. A. Sinclair and J. J. Robinson. 2000. Nitrogen metabolism and fertility in cattle: In adaptive changes in intake and metabolism to diet differing in their rate of energy release in the rumen. J. Anim. Sci. 78:2659-2669 https://doi.org/10.2527/2000.78102659x
  29. Sommart, K. 1998. The use of cassava in ruminant diets based on low quality roughages. Ph.D. Thesis, University of Newcastle upon Tyne
  30. Stern, M. D. and W. H. Hoover. 1979. Methods for determining and factors affecting rumen microbial protein synthesis: a review. J. Anim. Sci. 49:1590-1603 https://doi.org/10.2527/jas1979.4961590x
  31. Trevaskis, L. M., W. J. Fukerson and J. M. Gooden. 2001. Provision of certain carbohydrate based supplements to pasture fed sheep as well as time of harvesting of the pasture influences pH, ammonia concentration and microbial protein synthesis in the rumen. Aust. J. Exp. Agric. 41:21-27 https://doi.org/10.1071/EA00063
  32. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and Non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597 https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  33. Vongsamphan, P. and M. Wanapat. 2004. Effect of levels of cassava hay (CH) supplementation in native beef cattle feed on rice straw. In: Proceeding of the Agricultural Seminar, Animal Science/Animal husbandry. Held at Sofitel Rajaorcid hotel 27- 28 January 2004, 255-270
  34. Witt, M. W., L. A. Sinclair, R. G. Wikinson and P. J. Buttery. 1999. The effects of synchronizing the rate of dietary energy and nitrogen supply to the rumen on the production and metabolism of sheep food characterization and growth and metabolism of ewe lams given food ad libitum. Anim. Sci. 69:223-235 https://doi.org/10.1017/S1357729800051262
  35. Zinn, R. A., L. S. Bull and R. W. Hemken. 1981. Degradation of supplemental proteins in the rumen. J. Anim. Sci. 52:857-865 https://doi.org/10.2527/jas1981.524857x
  36. Zinn, R. A. and F. N. Owens. 1986. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Can. J. Anim. Sci. 66:157-166 https://doi.org/10.4141/cjas86-017

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