Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Association between Polymorphisms of Lipoprotein Lipase Gene and Chicken Fat Deposition

  • Liu, Rui (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University) ;
  • Wang, Yachun (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University) ;
  • Sun, Dongxiao (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University) ;
  • Yu, Ying (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University) ;
  • Zhang, Yuan (Department of Animal Genetics and Breeding, College of Animal Science and Technology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture China Agricultural University)
  • Received : 2006.02.02
  • Accepted : 2006.04.12
  • Published : 2006.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of this study was to screen single nucleotide polymorphisms (SNPs) of the chicken lipoprotein lipase gene (LPL), using 545 F1 hybrids developed from $4{\times}4$ diallel crossing of four chicken breeds, and to analyze the associations between polymorphisms of the LPL and chicken fat deposition traits. PCR-SSCP was used to detect SNPs in LPL. Fifteen sets of primers were designed to amplify DNA fragments covering the 5'flanking and coding regions of LPL. It showed that there existed 5 polymorphic loci in the 5'flanking region and coding region, respectively. Association analysis was carried out between 10 polymorphic loci and intermuscular fat width, abdominal fat weight, and thickness of subcutaneous fat using ANCOVA, respectively. The results indicated that, in the 5'flanking region, the loci d and e significantly affected thickness of subcutaneous fat (p<0.05), abdominal fat weight (p<0.01) and subcutaneous fat (p<0.05), while in the coding region, synonymous mutation in exon 8 was significantly associated with intermuscular fat width (p<0.05), however, the non-synonymous mutations in exon 7 and exon 9 did not show statistically significant effects on fat deposition traits in this study.

Keywords

References

  1. Choi, C. H., B. W. Cho, G. J. Jeon and H. K. Lee. 2006. Identification of Novel SNPs with Effect on Economic Traits in Uncoupling Protein Gene of Korean Native Chicken. Asian-Aust. J. Anim. Sci. 19(8):1065 https://doi.org/10.5713/ajas.2006.1065
  2. Cooper, D. A., S. C. Lu and R. Viswanath. 1992. The structure and complete nucleotide sequence of the avian lipoprotein lipase gene. Biochimica et Biophysica Acta 1129:166-171 https://doi.org/10.1016/0167-4781(92)90482-F
  3. Hermier, D. Q. B. A., I. Dugail and G. Guy. 1989.Evidence of enhanced storage capacity in adipose tissue of genetically fat chickens. J. Nutr. 119:1369-1375 https://doi.org/10.1093/jn/119.10.1369
  4. Hermier, D., M. R. Salichon and C. C. Whitehead. 1991. Relationships between plasma lipoproteins and glucose in fasted chickens selected for leanness or fatness by three criteria. Reprod. Nutr. Devel. 31(4):419-429 https://doi.org/10.1051/rnd:19910408
  5. James, R. Mead, Scott A. Irvine and Dipak P. Ramji. 2002. Lipoprotein lipase: structure, function, regulation, and role in disease. J. Mol. Med. 80:753-769 https://doi.org/10.1007/s00109-002-0384-9
  6. Lei, M. G., Y. Z. Xiong, C. Y. Deng, Z. F. Wu, I. Harbitz, B. Zuo and L. H. Dai. 2004. Sequence variation in the porcine lipoprotein lipase gene. Anim. Genet. 35(5):422-423 https://doi.org/10.1111/j.1365-2052.2004.01185.x
  7. Li, C. C., K. Li and J. Li. 2006. Polymorphism of ghrelin gene in twelve chinese indigenous chicken breeds and its relationship with chicken growth traits. Asian-Aust. J. Anim. Sci. 19(2):153
  8. Li, Chun-Yu and Li Hui. 2006. Association of MC4R gene polymorphisms with growth and body composition traits in chicken. Asian-Aust. J. Anim. Sci. 19(6):763 https://doi.org/10.5713/ajas.2006.763
  9. Lu, S. C. and A. Bensadoun. 1993. Identification of the 5'regulatory elements of avian lipoprotein lipase gene: synergistic effect of multiple factors. Biochimica et Biophysica Acta. 1216:375-384 https://doi.org/10.1016/0167-4781(93)90004-W
  10. Meng, H., J. G. Zhao, Z. H. Li and H. Li. 2005. Single nucleotide polymorphisms on peroxisome proliferator-activated receptor genes associated with fatness traits in chicken. Asian-Aust. J. Anim. Sci. 18(9):1221 https://doi.org/10.5713/ajas.2005.1221
  11. Merkel, M., R. H. Eckel and I. J. Goldberg. 2002. Lipoprotein lipase: genetics, lipid uptake, and regulation. J. Lipid Res. 43(12):1997-2006 https://doi.org/10.1194/jlr.R200015-JLR200
  12. Sato, K., Y. Akiba, Y. Chida and K. Takahashi. 1999. Lipoprotein hydrolysis and fat accumulation in chicken adipose tissues are reduced by chronic administration of lipoprotein lipase monoclonal antibodies. Poult. Sci. 78(9):1286-1291 https://doi.org/10.1093/ps/78.9.1286
  13. Sendak, R. A., K. Melford, A. Kao and A. Bensadoun. 1998. Identification of the epitope of a monoclonal antibody that inhibits heparin binding of lipoprotein lipase:new evidence for a carboxyl-terminal heparin-binding domain. J. Lipid Res. 39(3):633-646
  14. Zhang, W. and A. Bensadoun. 1999. Identification of a silencing element in the chicken lipoprotein lipase gene promoter: characterization of the silencer-binding protein and delineation of its target nucleotide sequence. Biochimica et Biophysica Acta. 1436:390-404 https://doi.org/10.1016/S0005-2760(98)00148-9

Cited by

  1. gene and its association with fatness traits in native and Cherry Valley Peking ducks vol.39, pp.5, 2008, https://doi.org/10.1111/j.1365-2052.2008.01761.x
  2. Genome-Wide Patterns of Genetic Variation in Two Domestic Chickens vol.5, pp.7, 2013, https://doi.org/10.1093/gbe/evt097
  3. Identification of SNPs of the L-BABP and L-FABP and their Association with Growth and Body Composition Traits in Chicken vol.50, pp.4, 2013, https://doi.org/10.2141/jpsa.0130005
  4. Genetic effects of polymorphisms in the prolactin receptor gene on chicken reproductive traits vol.53, pp.10, 2013, https://doi.org/10.1071/AN12178
  5. Physiological and pathophysiological aspects of peroxisome proliferator-activated receptor regulation by fatty acids in poultry species vol.72, pp.03, 2016, https://doi.org/10.1017/S0043933916000490
  6. Postnatal Expression Pattern of Adipose Type Fatty Acid Binding Protein in Different Adipose Tissues of Porcine vol.20, pp.6, 2006, https://doi.org/10.5713/ajas.2007.811
  7. Regulation of Lipoprotein Lipase by Fasting in Epididymal and Mesenteric Adipocytes of Rats vol.21, pp.5, 2006, https://doi.org/10.5713/ajas.2008.60603