Asian-Australasian Journal of Animal Sciences

  • 제19권11호
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  • Pages.1574-1579
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  • 2006
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Development of Bovine Nuclear Transfer Embryos Using Life-span Extended Donor Cells Transfected with Foreign Gene

  • Hwang, Seongsoo (Institute of Genetic Engineering, Hankyong National University) ;
  • Choi, Eun Joo (Institute of Genetic Engineering, Hankyong National University) ;
  • You, Seungkwon (College of Life and Environmental Sciences, Korea University) ;
  • Choi, Yun-Jaie (School of Agricultural Biotechnology, Seoul National University) ;
  • Min, Kwan-Sik (The Graduate School of Bio- and Information Technology) ;
  • Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
  • 투고 : 2005.12.26
  • 심사 : 2006.04.05
  • 발행 : 2006.11.01
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초록

This study was performed to determine the developmental potentials of nuclear transfer (NT) embryos using life-span extended cells transfected with a foreign gene as donor cells. A life-span extended bovine embryonic fibroblast cell line was transfected with an expression vector in which the human type II collagen (BOMAR) and ear fibroblasts were used as a donor cell. Cytogenetic analysis was performed to analyze the chromosomal abnormality of donor cells. The fusion rate of 1.8 kV/cm for $15{\mu}sec$ given twice was significantly higher than that of other groups (p<0.05) and the embryos lysed were significantly higher after 1.8 kV/cm for $20{\mu}sec$ given once compared to other groups (p<0.01). The blastocyst development in the ear cell group was statistically significant compared to both BOMAR groups (p<0.01). Both BOMAR groups cultured more than 40 passages (>40 passages) had a lower number of chromosomes; however, fresh granulosa cell (GC) and BOMAR groups cultured less than 20 passages had normal chromosome numbers. Both >40 passages BOMAR groups had numerous obscure debris in metaphase spreads. The transfected foreign gene was expressed in all BOMAR groups, but not in the GC group. Based on these results, the lower developmental potential of NT embryos using life-span extended donor cells transfected with a foreign gene might be a cause of chromosomal abnormality in donor cells.

키워드

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피인용 문헌

  1. Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts vol.20, pp.10, 2006, https://doi.org/10.5713/ajas.2007.1510
  2. Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection vol.21, pp.5, 2006, https://doi.org/10.5713/ajas.2008.70400
  3. Recent Progress in Biotechnology-based Gene Manipulating Systems to Produce Knock-In/Out Mouse Models vol.21, pp.5, 2006, https://doi.org/10.5713/ajas.2008.r.04
  4. Production of Bovine Nuclear Transfer Embryos Using Fibroblasts Transfected with Single-Chain Human Follicle-Stimulating Hormone Gene vol.22, pp.2, 2006, https://doi.org/10.5713/ajas.2009.80225