Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Egg Yolk Antibodies Produced in Response to Different Antigenic Fractions of E. coli O157:H7 on E. coli Suppression

  • Chae, H.S. (Product and Utility Division, National Livestock Research Institute) ;
  • Singh, N.K. (Product and Utility Division, National Livestock Research Institute) ;
  • Ahn, C.N. (Product and Utility Division, National Livestock Research Institute) ;
  • Yoo, Y.M. (Product and Utility Division, National Livestock Research Institute) ;
  • Jeong, S.G. (Product and Utility Division, National Livestock Research Institute) ;
  • Ham, J.S. (Product and Utility Division, National Livestock Research Institute) ;
  • Kim, D.W. (Product and Utility Division, National Livestock Research Institute)
  • Received : 2006.01.10
  • Accepted : 2006.05.04
  • Published : 2006.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of this research was to provide the characterization and method for producing anti-E. coli O157:H7 antibodies in egg-laying hens and to determine if the antibody can restrain the proliferation of E. coli O157:H7 in-vitro. Selected antigenic fractions (whole cell, outer membrane protein and lipopolysaccharide (LPS)) from E. coli O157:H7 were injected to hens in order to produce anti-E. coli O157:H7 antibodies. The immune response and the egg yolk antibodies of laying hens against the whole cell, outer membrane protein and LPS antigens were monitored by ELISA. The level of antibodies against whole cell antigen monitored through ELISA sharply increased after the initial immunization, and it was found to be maximum on day 49 however, the level was maintained up to day 70. Antibodies (5 mg/ml) directed against the whole cell inhibited E. coli proliferation 10-13 times more than outer membrane protein or LPS. The antibody response against the whole cell antigens appeared to have higher activity in restraining the proliferation of E. coli O157:H7 than antibody against outer membrane protein or LPS. Results reflected that increasing the IgY's in the egg yolk could prevent greater economic losses due to human and animal health from pathogenic bacteria i.e. E. coli O157:H7.

Keywords

References

  1. Akita, E. M. and E. C. Y. Li Chan. 1998. Isolation of bovine immunoglobulin G subclasses from milk,colostrums, and whey using immobilized egg yolk antibodies. J. Dairy Sci. 81(1):54-63 https://doi.org/10.3168/jds.S0022-0302(98)75550-X
  2. Akita, E. M. and S. Nakai. 1992. Immunoglobulins from egg yolk: isolation and purification. J. Food Sci. 57:629-634 https://doi.org/10.1111/j.1365-2621.1992.tb08058.x
  3. Akita, E. M. and S. Nakai. 1993. Comparision of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain. J. Immunol. Methods 160:207-214 https://doi.org/10.1016/0022-1759(93)90179-B
  4. Al-Haddad, S. 1999. Psoriasin (S100A7) expression and invasive breast cancer. Am. J. Path. 155(6):2057-2066 https://doi.org/10.1016/S0002-9440(10)65524-1
  5. Baldwin, T. J., L. A. Collins and J. C. Newton. 1997. Antigens of Edwardsiella icataluri recognised by serum antibodies from naturally infected channel catfish. Fish and Shellfish Immun. 7:261-271 https://doi.org/10.1006/fsim.1997.0081
  6. Barekamp, S., J. R. S. Munson and D. M. Jr. Granoff. 1981. Subtyping isolate of Haemophilus influenzae type b by outer menbrane protein profiles. J. Infect. Dis. 143:668-676 https://doi.org/10.1093/infdis/143.5.668
  7. Benkirane, R. 1998. Immunochemical characterization of an IgGbinding protein of Streptococcus suis. FEMS Immunol. Med. Microbiol. 20(2):121-127 https://doi.org/10.1111/j.1574-695X.1998.tb01118.x
  8. Chang, H. M., Y. C. Lee, C. C. Chen and Y. Y. Tu. 2002. Microencapsulation protects immunoglobulin in yolk(IgY) specific against Helicobacter pylori urease. J. Food Sci. 67(1):15-20 https://doi.org/10.1111/j.1365-2621.2002.tb11351.x
  9. Cipolla, A., J. Cordeviola, H. Terzolo, G. Combessies, J. Bardon, R. Noseda, A. Martinez, D. Medina, C. Morsella and R. Malena. 2001. Campylobacter fetus diagnosis: direct immunofluorescence comparing chicken IgY and rabbit IgG conjugates. Altex-Alternativen Zu Tierex-perimenten 18(3):165-170
  10. Di, L. A. D. 2001. Egg yolk antibodies against the E7 oncogenic protein of human papillomavirus type 16. Archi. Virol. 146(1):117-125 https://doi.org/10.1007/s007050170195
  11. Evelyn, A. D., T. Brad, W. C. Bosworth, J. R. Crayand and W. M. Harley. 1997. Pathogenicity of Escherichia coli O157:H7 in the intestines of neonatal calves. Infec. Immun. May:1842-1848
  12. Gast, R. K. and C. W. Beard. 1991. Detection of salmonella serogroup D-specific antibodies in the yolks of eggs laid by hens infected with salmonella enteritidis. Poult. Sci. 70(5):1273-1276 https://doi.org/10.3382/ps.0701273
  13. Gast, R. K., J. Guard-Petter and P. S. Holt. 2002. Characteristics of salmonella enteritis contamination in eggs after oral, aerosol, and intravenous inoculation of laying hens. Avian Dis. 46(3):629-635 https://doi.org/10.1637/0005-2086(2002)046[0629:COSECI]2.0.CO;2
  14. Gurtler, M., U. Methner, H. Kobilke and K. Fehlhaber. 2004. Effect of orally administered egg yolk antibodies on salmonella enteritidis contamination of Hen's eggs. J. Vet. Med. B. Infect Dis. Vet. Public Health 51(3):129-134 https://doi.org/10.1111/j.1439-0450.2004.00739.x
  15. Johnson, K. G. and M. B. Perry. 1976. Improved techniques for the preparation of bacterial LPS. Can. J. Microbiol. 22:29-34 https://doi.org/10.1139/m76-004
  16. Karmaill, M. A. 1989. Infection by verocytotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2:15-38 https://doi.org/10.1128/CMR.2.1.15
  17. Lee, E. N., H. H. Sunwoo, K. Menninen and J. S. Sim. 2002. In vitro studies of chicken egg yolk antibody (IgY) against salmonella enteritidis and salmonella typhimurium. Poult. Sci. 81:632-641 https://doi.org/10.1093/ps/81.5.632
  18. Li, X., T. Nakano, H. H. Sunwoo, B. H. Paek, H. S. Chae and J. S. Sim. 1998. Effects of egg and yolk weights on yolk antibody (IgY) production in laying chickens. Poult. Sci. 77:266-270 https://doi.org/10.1093/ps/77.2.266
  19. Mancini, G., A. O. Carbonora and T. F. Heremans. 1965. Immunochemical quantification of antigen by single radial immunodiffusion. Immuno-chem. 2:235-242 https://doi.org/10.1016/0019-2791(65)90004-2
  20. Penny, J. and Hitchcock. 1984. Analyses of gonococcal lipopolysaccharide in whole-cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Stable association of lipopoly-saccharide with the major outer membrane protein (protein I) of Neisseria gonorrhoeae. Infect. Immun. Oct:202-212. Press
  21. Rose, M. E. and E. Orlans. 1981. Immunoglobulins in the egg, embryo and young chick. Dev. Comp. Immun. 5:15-20, 315-319 https://doi.org/10.1016/S0145-305X(81)80003-1
  22. SAS Institute Inc. 2002. JMP, A Business Unit of SAS. Version 5, First edition, SAS Institute., SAS Campus Drive, Cary, NC 27513, USA
  23. Shimizu, M., S. Nakai and R. C. Fitzsimmons. 1988. An E.coli immunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient. J. Food Sci. 5:1360-1366
  24. Sim, S. J. and H. H. Sunwoo. 2003. Egg antibody farming and IgY technology for food and biomedical applications. Nutritional reevaluation of egg and chicken meat, International Symposium Proceedings of Korean Society of Poultry Science. 39-48
  25. Sunwoo, H. H., T. Nakano, W. T. Dixon and J. S. Sim. 1996. Immune responses in chickens against lipopolysaccharide of Escherichia coli and salmonella typhimurium. Poult. Sci. 75:342-345 https://doi.org/10.3382/ps.0750342
  26. Thomas, J. B., A. C. Lisa and C. N. Joseph. 1997. Antigens of edwardsiella icataluri recognized by serum antibodies from naturally infected channel catfish. Fish and Shellfish Immunology. 7:261-271 https://doi.org/10.1006/fsim.1997.0081
  27. Westphal, O. and K. Jann. 1965. Bacterial lipopolysaccharide extraction with phenol water and further applications of the procedure. In Methods in Carbohydrate Chemistry (Ed. R. L. Whister), 5:83-96. New York, New York: Academic

Cited by

  1. Sensitivity of Escherichia coli to Seaweed (Ascophyllum nodosum) Phlorotannins and Terrestrial Tannins vol.22, pp.2, 2006, https://doi.org/10.5713/ajas.2009.80213