Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Morphometric and Ultrastructural Change of Myelin-Associated Glycoprotein (MAG)-Immunoreactive Oligodendrocytes by Aging

노화에 의한 Myelin-Associated Glycoprotein (MAG) 면역반응 희소돌기아교세포의 형태계측학적 및 미세구조적 변화

  • Cho, Ik-Hyun (College of Veterinary Medicine, Konkuk University) ;
  • Park, Chang-Hyun (Electron Microscope Facility, College of Medicine, Korea University) ;
  • Lee, Jong-Hwan (College of Veterinary Medicine, Konkuk University) ;
  • Bae, Chun-Sik (College of Veterinary Medicine and Biotechnology Research Institutes, Chonnam National University) ;
  • Ye, Sang-Kyu (Department of Pharmacology, Seoul National University Collge of Medicine) ;
  • Lee, Beob-Yi (Department of Anatomy, College of Medicine, Konkuk University) ;
  • Park, Seung-Hwa (Department of Anatomy, College of Medicine, Konkuk University) ;
  • Koh, Ki-Seok (Department of Anatomy, College of Medicine, Konkuk University) ;
  • Kim, Jin-Suk (College of Veterinary Medicine, Konkuk University) ;
  • Chang, Byung-Joon (College of Veterinary Medicine, Konkuk University)
  • 조익현 (건국대학교 수의과대학) ;
  • 박창현 (고려대학교 의과대학 전자현미경실) ;
  • 이종환 (건국대학교 수의과대학) ;
  • 배춘식 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 예상규 (서울대학교 의과대학 약리학교실) ;
  • 이법이 (건국대학교 의과대학 해부학교실) ;
  • 박승화 (건국대학교 의과대학 해부학교실) ;
  • 고기석 (건국대학교 의과대학 해부학교실) ;
  • 김진석 (건국대학교 수의과대학) ;
  • 장병준 (건국대학교 수의과대학)
  • Published : 2006.06.30
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Abstract

To investigate the role of myelin-associated glycoprotein (MAG) in the normal aging process, aging-related morphometric and ultrastructural analyses of the MAG-positive (MAG-(+)) oligodendrocytes were carried out in the cerebral cortex of the Sprague-Dawley rats. In the aged rats, the density of MAG-(+) oligodendrocytes was significantly decreased in the cortical layer (IV-VI) compared with that of the adult rats. However, the percentage of medium and dark types of oligodendrocytes was significantly increased by aging. In the aged rats, the mean nuclear area of the MAG-(-) oligodendrocytes was interestingly reduced compared with that of MAG-(+) oligodendrocytes. In addition, MAG immunoreactive products were markedly decreased in the medium-dark type of oligodendroglial cytoplasm and processes, and were scarcely localized in the dark type of oligodendrocytes of the aged rats. These results suggest that degeneration of oligodendrocytes-myelin system by aging is associated with down regulation of MAG, and that may contribute to further understanding of the biology of MAG in the oligodendrocytes-myelin system.

정상적인 노화과정에 있어서 마이엘린연합당단백질(MAG)의 기능을 알아보고자 Sprague-Dawley 계통 흰쥐의 대뇌피질에서 MAG 면역양성반응세포(희소돌기아교세포)에 대한 형태계측학적 및 미세구조적인 분석을 시행하였다. 노화된 흰쥐의 대뇌피질(IV-VI)에서 MAG면역양성반응세포의 밀도는 정상 흰쥐에 비하여 유의하게 감소하였다. 그러나 medium과 dark 형의 희소돌기아교세포의 비율은 증가하였다. 노화된 흰쥐의 대뇌피질에서 MAG면역음성반응세포의 핵의 평균면적은 MAG면역양성반응세포의 핵의 평균면적보다 유의하게 감소하였다. MAG의 면역반응물은 노화된 흰쥐의 대뇌피질의 medium-dark형의 희소돌기아교세포의 세포질과 돌기에서 뚜렷이 감소하였고 dark형의 희소돌기아교세포에서는 거의 관찰되지 않았다. 이러한 결과는 노화에 의한 희소돌기아교세포와 마이엘린의 변성은 MAG의 감소와 관계가 있을 것으로 생각되며, 희소돌기아교세포와 마엘린 계통에서 MAG의 기능을 연구하는 기초자료로서 유용할 것으로 사료된다.

Keywords

References

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