Advances in Traditional Medicine

  • 제6권4호
  • /
  • Pages.293-299
  • /
  • 2006
  • /
  • 2662-4052(pISSN)
  • /
  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
권호 표지
DOI QR코드

DOI QR Code

Regions in China identification and quality control of radix Codonopsis by chemical fingerprint: Evaluation of lobetyolin from different cultivated

  • Chou, Gui X (Department of Biology, Hong Kong University of Science and Technology) ;
  • Gao, Qiu T (Department of Biology, Hong Kong University of Science and Technology) ;
  • Li, Jun (Department of Biology, Hong Kong University of Science and Technology) ;
  • Duan, Ran (Department of Biology, Hong Kong University of Science and Technology) ;
  • Cheung, Anna WH (Department of Biology, Hong Kong University of Science and Technology) ;
  • Chu, Glanice KY (Department of Biology, Hong Kong University of Science and Technology) ;
  • Jiang, Zhi Y (Department of Biology, Hong Kong University of Science and Technology) ;
  • Dong, Tina TX (Department of Biology, Hong Kong University of Science and Technology) ;
  • Tsim, Karl WK (Department of Biology, Hong Kong University of Science and Technology)
  • 발행 : 2006.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

By using high-performance liquid chromatography-photodiode array detection, a simple and accurate chromatographic fingerprint method was developed for the identification of Radix Codonopsis (roots of Codonopsis) from different sources. Eighteen herbs of Codonopsis at different habitats in China, including roots from Codonopsis pilosula, Codonopsis pilosula var. modesta and Codonopsis tangshen were analyzed by the fingerprint. The amount of lobetyolin was calibrated, which was found to be more consistent in roots of Codonopsis pilosula as compared to that of Codonopsis pilosula var. modesta and Codonopsis tangshen. Having the fingerprint results, hierarchical clustering analyses were performed to classify the eighteen herbs into three groups: Codonopsis pilosula, Codonopsis pilosula var. modesta and Codonopsis tangshen. This clustering analysis agrees very well with the pharmacognostic identification result, and which could be used as a tool in the quality control of Radix Codonopsis.

키워드

참고문헌

  1. European Medicines Agency. (2001) Note for Guidance on Quality of Herbal Medicinal Products, pp. 6, London.
  2. Gu M, Ouyang F, Su ZG. (2003) Comparison of high-speed counter-current chromatography and high-performance liquid chromatography on fingerprinting of Chinese traditional medicine. J. Chromatogr. A 1022, 139-144. https://doi.org/10.1016/j.chroma.2003.09.038
  3. Jin ZL,Wen J, Li SF. (1996) Anti-aging effect of shenqi mixture tonic. Chin. Trad. Herb Drugs 27, 90-92.
  4. Liu JH, Bao YM, Song JJ, An LJ. (2003) Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in PC12 cells. Acta Pharmacol. Sin. 24, 913-917.
  5. Lu GH, Chan K, Liang YZ, Leung K, Chan CL, Jiang ZH, Zhao ZZ. (2005) Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs J. Chromatogra. A 1073, 383-392. https://doi.org/10.1016/j.chroma.2004.11.080
  6. Ng TB, Liu F, Wang HX. (2004) The antioxidant effects of aqueous and organic extracts of Panax quinquefolium, Panax notoginseng, Codonopsis pilosula, Pseudostellaria heterophylla and Glehnia littoralis. J. Ethnopharmacol. 93, 285-288. https://doi.org/10.1016/j.jep.2004.03.040
  7. Pan SY. (1992) Circadian effects of scopolamine on memory, exploratory behavior, and muscarinic receptors in mouse brain. Acta Pharmacol. Sin. 13, 323-326.
  8. Singh B, Song H, Liu XD, Hardy M, Liu GZ, Vinjamury SP, Martirosian CD. (2004) Dangshen (Codonopsis pilosula) and Bai guo (Gingko biloba) enhance learning and memory. Altern. Ther. Health Med. 10, 52-56.
  9. State Food and Drug Administration of China. (2000) Note for Studying Fingerprint of Traditional Chinese Medicine Injections, pp. 3, Shanghai.
  10. US Food and Drug Administration. (2000) FDA Guidance for Industry - Botanical Drug Products, pp. 4, Rockville.
  11. Wang ZT, Du Q, XU GJ, Wang RJ, Fu DZ, Ng TB. (1997) Investigations on the protective action of Condonopsis pilosula (Dangshen) extract on experimentally-induced gastric ulcer in rats. Gen. Pharmacol. 28, 469-473.
  12. Xia Q, Zhao KJ, Huang ZG, Zhang P, Dong, TTX, Li SP, Tsim KWK. (2005) Molecular genetic and chemical assessment of Rhizoma Curcumae in China. J. Agric. Food Chem. 53, 6019-6026. https://doi.org/10.1021/jf0508495
  13. Yan SK, Xin WF, Luo GA, Wang YM, Cheng YY. (2005) An approach to develop two-dimensional fingerprint for the quality control of Qingkailing injection by high-performance liquid chromatography with diode array detection. J. Chromatogra. A 1090, 90-97. https://doi.org/10.1016/j.chroma.2005.07.066
  14. Zhao KJ, Dong TTX, Tsim KWK. (2003) Molecular genetic and chemical assessment of Radix Angelica (Danggui) in China. J. Agric. Food Chem. 51, 2576-2583. https://doi.org/10.1021/jf026178h
  15. Zheng TZ, Li W, Qu SY, Ma YM, Ding YH, Wei YL. (1998) Effects of Dangshen on isolated gastric muscle strips in rats. World J. Gastroenterol. 4, 354-356.
  16. Zheng XY. (2005) Pharmacopoeia of the People's Republic of China, pp. 199, Chemistry Industry Press: Beijing, China.
  17. Zou P, Hong Y, Koh HL. (2005) Chemical fingerprinting of Isatis indigotica root by RP-HPLC and hierarchical clustering analysis, J. Pharm. Biomed. Anal. 38, 514-520. https://doi.org/10.1016/j.jpba.2005.01.022