The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Involvement of Endoplasmic Reticulum Stress Response in the Neuronal Differentiation

  • Cho, Yoon-Mi (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Jang, Yoon-Seong (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Jang, Young-Min (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Seo, Jin-Young (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Kim, Ho-Shik (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Lee, Jeong-Hwa (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Jeong, Seong-Whan (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Kim, In-Kyung (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Kwon, Oh-Joo (Department of Biochemistry, MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea)
  • Published : 2007.12.31
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Abstract

Expressions of endoplasmic reticulum stress response (ERSR) genes were examined during the neuronal differentiation of rat fetal cortical precursor cells (rCPC) and rat pheochromocytoma PC12 cells. When rCPC were differentiated into neuronal cells for 7 days, early stem cell marker, nest in, expression was decreased from day 4, and neuronal markers such as neurofilament-L, -M and Tuj1 were increased after day 4. In this condition, expressions of BIP, ATF6, and phosphorylated PERK as well as their down stream signaling molecules such as CHOP, ATF4, XBP1, GADD34, Nrf2 and $p58^{IPK}$ were significantly increased, suggesting the induction of ERSR during neuronal differentiation of rCPC. ERSR was also induced during the differentiation of PC12 cells for 9 days with NGF. Neurofilament-L transcript was time-dependently increased. Both mRNA and protein levels of Tuj1 were increased after the induction, and the significant increase in NeuN was observed at day 9. Similar to the expression patterns of neuronal markers, BIP/GRP78 and CHOP mRNAs were highly increased at day 9, and ATF4 mRNA was also increased from day 7. These results strongly suggest the induction and possible role of ERSR in neuronal differentiation process. Further study to identify targets responsible for neuronal induction will be necessary.

Keywords

References

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