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Production of Cloned Korean Native Goat (Capra hircus) by Somatic Cell Nuclear Transfer

  • Park, H.S. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Jung, S.Y. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Kim, T.S. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Park, J.K. (Gyeongnam Province Advanced Swine Research Institute) ;
  • Moon, T.S. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Hong, S.P. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Jin, J.I. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Lee, J.S. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Lee, J.H. (EASY BIO System, Inc.) ;
  • Sohn, S.H. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Lee, C.Y. (Regional Animal Industry Center, Jinju National University) ;
  • Moon, Y.S. (Department of Animal Science and Biotechnology, Jinju National University)
  • 투고 : 2006.07.25
  • 심사 : 2006.11.17
  • 발행 : 2007.04.01

초록

The objectives of the present study were to initiate cloning of Korean native goat by somatic cell nuclear transfer (NT) and to examine whether unovulated (follicular) oocytes can support the same developmental ability of NT embryos as ovulated (oviductal) oocytes after hCG injection in stimulated cycles of the goat. The in vivo-matured and immature oocytes were collected from the oviducts and follicles of superovulated does, respectively, and the immature oocytes were maturated in vitro. Ear skin fibroblasts derived from a 3-yr-old female Korean native goat were used as the donors of nuclei or karyoplasts. Following fusion, activation and in vitro culture to a 2- to 4-cell stage, 49 in vitro-derived and 105 in vivo-derived embryos were transferred to 6 and 17 recipient does, respectively. One doe and three does of the respective groups were identified as pregnant by ultrasonography on day 30 after embryo transfer. However, only one doe, which had received in vivo-derived embryos, delivered a normal female kid of 1.9 kg on d 149. The cloned kid gained more weight than her age-matched females as much as 87% during the first 4 mo after birth (17.7 vs. $9.4{\pm}0.8$ kg) and reached puberty at 6-mo age a few months earlier than normal female does. The telomere length of the kid, which was similar to that of the donor fibroblast at 2-mo age, decreased 8% between 2- and 7-mo ages. Moreover, at 7-mo age, she had 21% shorter telomere than her age-matched goats. To our knowledge, this is the first case in which a cloned animal born with a normal weight exhibited accelerated growth and development. The unusually rapid growth and development of the cloned goat may have resulted from SCNT-associated epigenetic reprogramming involving telomere shortening.

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참고문헌

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