방풍갈근탕(防風葛根湯)의 항알레르기효과에 관한 연구

Study on Anti-allergic Effect and Safety of Bangpung-galgeun-tang

  • 이주은 (동의대학교 한의과대학 생리학교실 한의학연구소) ;
  • 박성하 (동강한방병원) ;
  • 강경화 (동의대학교 한의과대학 생리학교실 한의학연구소) ;
  • 이용태 (동의대학교 한의과대학 생리학교실 한의학연구소)
  • Lee, Joo-Eun (Department of Physiology Research Institute of Oriental Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Park, Seong-Ha (Donggang Oriental Hospital) ;
  • Kang, Kyung-Hwa (Department of Physiology Research Institute of Oriental Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, Yong-Tae (Department of Physiology Research Institute of Oriental Medicine, College of Oriental Medicine, Dong-Eui University)
  • 발행 : 2007.10.25

초록

The purpose of this study was to examine the anti allergic effect in vivo and in vitro, and to observe single and four weeks repeated toxicity in mice of Bangpung-galgeun-tang (BGT). We investigated anti DNP IgE-mediated passive cutaneous anaphylaxis in rodents and compound 48/80-induced active systemic anaphylatic shock in mice after oral administration with BGT of 0.4 g/kg and 0.8 g/kg for 8 days, and also examined MTT assay, ${\beta}-hexosaminidase$ activity, IL-4 and $TNF-{\alpha}$ from RBL-2H3 and $TNF-{\alpha}$ from Raw264.7 after pre-treatment with BGT of 0.25 mg/ml, 0.5 mg/ml, 1 mg/ml and 2 mg/ml. To ascertain safety and toxicity of BGT, we divided into single and four weeks repeated administration test. In single test, three groups were administrated different dosages and routes (2 g/kg/i.p., 4 g/kg/i.p. and 15 g/kg /p.o.) of BGT, and in four weeks repeated test, 0.8 g/kg BGT was administrated. Control groups were administrated with only saline according to on Korean Food and Drug Administration, respectively. We observed attentively motality, abnormal clinical sign, body weight change, organ weight, AST and ALT of mice after BGT administration. BGT inhibited passive cutaneous anaphylaxis and active systemic anaphylatic shock by oral administration. All the concentrations of BGT from 0.25 to 2 mg/ml didn't have an effect on cell viability and cytotoxicity. In RBL-2H3, ${\beta}-hexosaminidase$ release, IL-4 and $TNF-{\alpha}$, and in Raw264.7, $TNF-{\alpha}$ were significantly reduced by treated all concentrations of BGT. During toxicity experiment period, there was no difference in body weight change, organ weight, AST and ALT among different dose groups. Death were found 3 mice from day 2 to day 3 in single test i.p. group. (2 g/kg, 4 g/kg). Several individuals of single test i.p. group were observed that decreased locomotor activity, exophthalmos, bloodshot eyes, loss of eyesight and so on in early period after administration. But there was no difference in clinical signs among p.o. group. These results indicate that BGT have inhibition effects on allergy and suggest that no observable effect level of the test orally administration was considered to be more than 2 g/kg in mice under the conditions employed in this study.

키워드

참고문헌

  1. 대한 천식 및 알레르기 학회. 천식과 알레르기 질환. 군자출판사, 66: 431-433, 2002
  2. 彭懷仁 主編. 中華醫方精選辭典. 上海, 上海科學技術文獻出版社, p 931, 1998
  3. S. Katayama, H. Shionoya, S. Ohtake. A new method for extraction of extravasated dye in the skin and the influence of fasting stress on passive cutaneous allergy in guinea pigs and rats. Microbiol. Immunol. 22: 89-101, 1978 https://doi.org/10.1111/j.1348-0421.1978.tb00352.x
  4. Dastych, J., Walczak-Drzewiecka, A., Wyczolkowska, J, Metcalfe, D.D. Murine mast cells exposed to mercuric chloride release granule-associated N-acetyl-beta-d-hexosaminidase and secrete IL-4 and TNF-alpha. J Allergy Clin Immunol. 103: 1108-1114, 1999 https://doi.org/10.1016/S0091-6749(99)70186-7
  5. Schwartz, L.B., Austen, K.F., Wasserman, S.I. Immunologic release of beta-hexosaminidase and beta-glucuronidase from purified rat serosal mast cells. J Immunol. 123: 1445-1450, 1979
  6. 식품의약품안전청. 의약품등의 독성시험기준. 식품의약품안전청 고시 2005-60호, 서울, 식품의약품안전청, 1999
  7. 국립독성연구원. 표준작업지침서. 서울, 국립독성연구원, 2007
  8. 은희철 외. 피부면역학. 서울대학교출판부, pp 1-2, 1999
  9. Halfon, N., Newacheck, P.W. Trends in the hospitalization for acute childhood asthma, 1970-84. Am J Public Health. 76(11):1308-1311, 1986 https://doi.org/10.2105/AJPH.76.11.1308
  10. Mitchell, E.A. Increasing prevalence of asthma in children. N Z Med J. 96(734):463-464, 1983
  11. Smith, J.M., Harding, L.K., Cumming, G. The changing prevalence of asthma in school children. Clin Allergy. 1(1):57-61, 1971 https://doi.org/10.1111/j.1365-2222.1971.tb02447.x
  12. 김유영, 조상헌, 김우경, 박재경, 김윤근, 송숙희, 지영구, 하미나, 안윤옥, 이상일, 장석일, 민경업. 설문지와 메타콜린 기관지유발시험을 이용하여 조사한 한국의 소아 천식 유병률. 대한알레르기학회지 16(2):175-184, 1996
  13. 黃兆勝 主編. 中藥學. 北京, 人民衛生出版社, p 42, 58, 59, 124, 426, 2005
  14. Ehrlich, P. Beitrage zur kenntnis der Anilinfarbunger und ihrer Verwendung in drmikroskopischen Technik. Arch Mikrosk Anast 13: 263-277, 1877 https://doi.org/10.1007/BF02933937
  15. Moon, P.D., Na, J.J., Jeong, H.J., Hong, S.H., Kim, S.J., Chae, H.J., Kim, H.R., Choi, J.O., Lee, S.H., Shin, J.Y., Kim, H.M. Inhibitory effect of Gamibojungikaitang extract on mast cell-mediated allergicreaction in murine Model. J Pharm Pharm Sci 8(1):94-101, 2005
  16. Ennis, M., Pearce, F.L., Weston, P.M. Some studies on the release of histamine from mast cells stimulated with polylysine. Br. J. Pharmacol 70: 329-334, 1980 https://doi.org/10.1111/j.1476-5381.1980.tb07940.x
  17. 강경진, 전병득, 채옥희, 이무삼. 상백피가 흰쥐 복강 비만세포의 히스타민 유리와 Calcium uptake에 미치는 영향. 대한면역학회지 15: 91-99, 1993
  18. 채옥희, 이종인, 이무삼. 상백피의 colchicine 유도 비만세포 활성화 억제효과. 대한해부학회지 32: 735-747, 1999
  19. B.S. Bochner, L.M. Lichtenstein. Anaphylaxis. N. Engl. J. Med. 324: 1785-1790, 1991 https://doi.org/10.1056/NEJM199106203242506
  20. Sheffer, A.L. Anaphylaxis: clinical aspects. Allergy Asthma Proc. 25(1):31-32, 2004
  21. Dainaka, J., Ichikawa, A., Koibuchi, Y., Nakagawa, M., Tomita, K. Effect of the tridecamer of compound 48/80, a $Ca^{2+}$ -dependent histamine releaser, on phospholipid metabolism during the early stage of histamine release from rat mast cells. Biochem Pharmacol. 35(21):3739-3744, 1986 https://doi.org/10.1016/0006-2952(86)90659-3
  22. Lane, S.J., Lee, T.H. Mast cell effector mechanisms. J Allergy Clin Immunol. 98(5 Pt 2):S67-71, 1996 https://doi.org/10.1016/S0091-6749(96)70019-2
  23. Jirapongsananuruk, O., Leung, D.Y. Clinical applications of cytokines: new directions in the therapy of atopic diseases. Ann Allergy Asthma Immunol. 79(1):5-20, 1997 https://doi.org/10.1016/S1081-1206(10)63078-5
  24. 정해영. 생명과학.분자의학을 위한 사이토카인 분자생물학. 월드사이언스, pp 117-124, 2002
  25. 이중달. 그림으로 설명한 병리학. 서울, 고려의학, p 29, 1991
  26. 강재성 외. 세포분자면역학. 서울, 범문사, p 283, 435, 2002
  27. Kim, J.C., Kim, S.H., Shin, D.H., Ahn, T.H., Kim, H.C., Kim, Y.B., Jiang, C.Z., Han, J., Chung, M.K. Effects of prenatal exposure to the environmental pollutant 2-bromopropane on embryo-fetal development in rats. Toxicology. 196(1-2): 77-86, 2004 https://doi.org/10.1016/j.tox.2003.11.006
  28. Kim, J.C., Shin, D.H., Kim, S.H., Kim, J.K., Park, S.C., Son, W.C., Lee, H.S., Suh, J.E., Kim, C.Y., Ha, C.S., Chung, M.K. Subacute toxicity evaluation of a new camptothecin anticancer agent CKD-602 administered by intravenous injection to rats. Regul Toxicol Pharmacol. 40(3):356-369, 2004 https://doi.org/10.1016/j.yrtph.2004.09.002