Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anticariogenic Properties of the Ethanol Extract of Tribuli fructus against Streptococcus mutans

백질려 추출물이 Streptococcus mutans에 대한 항치아우식에 미치는 영향

  • Lee, Da-Hong (Department of Food and Nutrition, Wonkwang University) ;
  • Yu, Hyeon-Hee (Department of Food and Nutrition, Kunsan National University) ;
  • Jung, Su-Young (Department of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • Moon, Hae-Dalma (Department of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • Kim, Su-Min (Department of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • You, Yong-Ouk (Department of Oral Biochemistry, School of Dentistry, Wonkwang University)
  • 이다홍 (원광대학교 식품영양학과) ;
  • 유현희 (군산대학교 식품영양학과) ;
  • 정수영 (원광대학교 치과대학 구강생화학교실) ;
  • 문해닮아 (원광대학교 치과대학 구강생화학교실) ;
  • 김수민 (원광대학교 치과대학 구강생화학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 유용욱 (원광대학교 치과대학 구강생화학교실)
  • Published : 2007.10.25
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Abstract

Streptococcus mutans is considered one of the primary etiologic agents of dental caries. we studied the effect of the ethanol extracts of Tribuli fructus (T. fructus) on the growth, biofilm formation, acid production, adhesion and insoluble glucan synthesis of S. mutans. The ethanol extracts of T. fructus showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.025, 0.05, 0.1, 0.2, 0.3, 0.5 mg/ml compared to the control group. In the biofilm assay, the ethanol extracts of T. fructus inhibited formation of biofilm synthesized by S. mutans at the concentration of 0.05 mg/ml. The extracts markedly inhibited S. mutans adherence to HA treated with saliva, and cell adherence was repressed by more than 50% at the concentration 0.05 mg/ml. On the activity of glucosyltransferase which synthesizes water insoluble glucan form sucrose, ethanol extract of T. fructus showed more than 10% inhibition over the concentration of 0.025 mg/ml. Hence, we conclude that T. fructus might be a candidate of anticaries agent. Further studies are necessary to clarify the active constituents of T. fructus responsible for such biomolecular activities.

Keywords

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