Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Analysis of Plasma Proteome before and after Oral Administration of Acidic Polysacharide from Panax Ginseng, Double-blind, Placebo-controlled, Randomised Trial

인삼 다당체 경구 투여 전 ${\cdot}$ 후 혈장의 proteome 분석;무작위배정, 이중 맹검, 위약비교시험

  • Lee, Seon-Goo (Department of Oriental Pathology College of Oriental Medicine, Sangji University) ;
  • Cho, Young-Ho (Department of Oriental Pathology College of Oriental Medicine, Sangji University) ;
  • Kang, Tae-Gyu (Department of Oriental Pathology College of Oriental Medicine, Sangji University) ;
  • Yang, Ha-Young (Department of Oriental Pathology College of Oriental Medicine, Sangji University) ;
  • Yoo, Byong-Chul (Research Institute, National Cancer center) ;
  • Cho, Chong-Kwan (East-West Cancer Center, College of Oriental Medicine, Daejeon University) ;
  • Yoo, Hwa-Seung (East-West Cancer Center, College of Oriental Medicine, Daejeon University)
  • 이선구 (상지대학교 한의과대학 병리학교실) ;
  • 조영호 (상지대학교 한의과대학 병리학교실) ;
  • 강태규 (상지대학교 한의과대학 병리학교실) ;
  • 양하영 (상지대학교 한의과대학 병리학교실) ;
  • 유병철 (국립암센터 연구소) ;
  • 조종관 (대전대학교 둔산한방병원 동서암센터) ;
  • 유화승 (대전대학교 둔산한방병원 동서암센터)
  • Published : 2007.10.25
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Abstract

Ginsing polysaccharide, known to have an immune regulating effect, was administered to 23 randomly selected healthy male subjects with a mean age of 23 years in accordance with an IRB approval. Then, these subjects underwent physico-chemical tests and serum proteome was analyzed from the blood sample taken from these subjects. Analyses of proteome involved image analysis, protein sections and protein identification in sequence after two-dimensional electrophoresis was carried out. During the physico-chemical test, 4 subjects were excluded from the study. In the proteome analysis, identified were 5 spots such as SP40, 40, Cytokeratin 9, hypothetical protein LOC544932, Apolipoprotein E ,similar to Human albumin, which showed differences in the amount of protein expression. In conclusion, changes of 5 proteins were remarkable before and after administration of ginsing polysaccharides. In certain cases, hepatic and renal slight injury occurred. Thus, further clinical study on dosage regimen would be necessary for securing the basis for concentration-dependent effectiveness and safety.

Keywords

References

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