Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Neuroprotective Effects of Rehmanniae Radix on PC12 Cells and Hippocampal Neural Cells

선지황(鮮地黃)이 PC12 세포 및 뇌해마 신경세포 손상에 미치는 영향

  • Jo, Jae-Heun (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Shin, Jung-Won (Department of Anatomy & Neurobiology, School of Medicine, Washington University in St.) ;
  • Shim, Eun-Shep (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Kim, Bum-Hoi (Department of Anatomy & Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Sohn, Young-Joo (Department of Gynecology, College of Oriental Medicine, Sangji University) ;
  • Jung, Hyuk-Sang (Department of Anatomy & Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Sohn, Nak-Won (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University)
  • 조재헌 (경희대학교 동서의학대학원 신경과학 및 뇌질환전공) ;
  • 신정원 (워싱턴대학교 의과대학 해부학 및 신경생물학교실) ;
  • 심은섭 (경희대학교 동서의학대학원 신경과학 및 뇌질환전공) ;
  • 김범회 (경희대학교 한의과대학 해부학교실 및 한의학연구소) ;
  • 손영주 (상지대학교 한의과대학 부인과학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실 및 한의학연구소) ;
  • 손낙원 (경희대학교 동서의학대학원 신경과학 및 뇌질환전공)
  • Published : 2007.12.25
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Abstract

The present study investigated neuroprotective effects Rehmanniae Radix on PC12 cells and hippocampal neural cells. PC12 cells were damage by $H_2O_2$ and nitric oxide and organotypic hippocampal slice cultures were damaged by oxygen-glucose deprivation. Then methanol extract of Rehmanniae Radix was treated with 0.5, 5, and $50\;{\mu}g/ml$ in culture media. Effects of Rehmanniae Radix were evaluated with cell viability assay, PI-staining, and TUNEL-labeling. Treatment of Rehmanniae Radix ($with\;5\;and\;50\;{\mu}g/ml$) produced significant increase of cell viability of PC12 cells damaged by $H_2O_2$ and by SNP-induced nitric oxide. Treatment of Rehmanniae Radix produced significant decrease of PI-uptake % in CA1 ($with\;5\;and\;50\;{\mu}g/ml$) and DG ($with\;50\;{\mu}g/ml$) regions of organotypic hippocampal slice cultures damaged by oxygen-glucose deprivation. Moreover, treatment of Rehmanniae Radix produced significant decrease of TUNEL- positive cells in CA1 ($with\;5\;and\;50\;{\mu}g/ml$) and DG ($with\;50\;{\mu}g/ml$) regions of organotypic hippocampal slice cultures damaged by oxygen-glucose deprivation. These results suggest that methanol extract of Rehmanniae Radix has neuroprotective effects on PC12 cells damaged by oxidative stress and on organotypic hippocampal slice cultures damaged by oxygen-glucose deprivation.

Keywords

References

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