상황버섯보리의 기능성에 관한 연구

Effects of Phellinus linteus-Barley corn Water Extract in Triton WR-1339(TX)-Induced Hiperlipidemic Rats

  • 석민희 (동의대학교 한의과대학 생리학교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실) ;
  • 최영현 (생화학교실, 동의대학교 한의학 연구소) ;
  • 최병태 (부산대학교 한의학전문대학원) ;
  • 박성하 (동강한방병원) ;
  • 이용태 (동의대학교 한의과대학 생리학교실)
  • Suk, Min-Hee (Department of Oriental Medicine, Dong-Eui University) ;
  • Kang, Kyung-Hwa (Department of Oriental Medicine, Dong-Eui University) ;
  • Choi, Young-Hyun (Department of Biochemistry, Research Institute of Oriental Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Choi, Byung-Tae (Department of Anatomy, Pusan Nationa University Graduate School of Oriental Medicine) ;
  • Park, Sung-Ha (Donggang Oriental Hospital) ;
  • 발행 : 2007.12.25

초록

This study was designed to observe the effects of the Phellinus linteus-Barley corn water extract (PLBC) on the improvement of the blood biochemical aspects; serum AST, ALT, LDH, ALP, Phospholipid, Trigryceride, Total Cholesterol, HDL, LDL, of Triton WR-1339(TX)-induced hiperlipidemic rats. Experimental groups were designed to normal control group(Normal), hiperlipidemic control group(Control), hiperlipidemic control group treated with Barley corn water extract(1000mg/1kg ; BC), hiperlipidemic control group treated with Phellinus linteus-Barley corn water extract (500, 1000, 1500mg/1kg; PLBC-1, PLBC-2, and PLBC-3, respectively). Serum AST levels of BC and all PLBC treated groups were significantly decreased (p<0.005) compared to Control group. Serum ALT levels of BC and PLBC-2 groups were decreased (p<0.05), PLBC-1 and PLBC-3 groups were significantly decreased (p<0.005) compared to Control group. Serum LDH levels of BC group was decreased but no significant, PLBC-1 and PLBC-2 groups were decreased (p<0.05), PLBC-3 group was significantly decreased (p<0.025) compared to Control group. Serum ALP levels of BC and PLBC-1 groups were decreased (p<0.05), PLBC-2 group was decreased but no significant, PLBC-3 group was significantly decreased (p<0.005) compared to Control group. Serum Phospholipid levels of BC group was decreased but no significant, PLBC-1 group was decreased (p<0.025), PLBC-2 and PLBC-3 groups were significantly decreased (p<0.005) compared to Control group. Serum Triglyceride levels of BC group was increased but no significant, PLBC-1 group was decreased but no significant, PLBC-2 and PLBC-3 groups were significantly decreased (p<0.005) compared to Control group. Serum Total cholesterol levels of BC group was decreased (p<0.05), PLBC-1, PLBC-2 and PLBC-3 groups were significantly decreased (p<0.005) compared to Control group. Serum HDL levels of BC group was increased (p<0.05), PLBC-1 and PLBC-2 groups were significantly increased (p<0.005), PLBC-3 group was increased (p<0.025) compared to Control group. Serum LDL levels of BC, PLBC-1 and PLBC-2 groups were decreased but no significant, PLBC-3 group was significantly decreased (p<0.005) compared to Control group. These results suggested that Phellinus linteus-Barley corn water extract improve Triton WR-1339(TX)-induced hiperlipidemic symptom in rats.

키워드

참고문헌

  1. 최재수. 고지혈증과 그 치료약. 생명과학 2(2):134-137, 1992
  2. 최재수, 양한석. 천연에 존재하는 고지혈증 개선 활성성분의 탐색. 생명과학 3(2):79-90, 1993
  3. Dalin, R., Hiroyuki, W., Hideomi, A., Takahiro, N. Study on Antihypertensive and Antihyperlipidemic effects of marine algaae. Fishersies Science. 60(1):83-88, 1994 https://doi.org/10.2331/fishsci.60.83
  4. Dominique, H., Paul, H., David, N.B. Effect of lipase inhibitor, triton WR-1339 and tetrahydrolipstatin, on the synthesis and secrtion of lipids by rat hepatocytes. FEBS. 286(1,2):186-188, 1991 https://doi.org/10.1016/0014-5793(91)80970-E
  5. Gerber, L.E., Erdman, J.W. Hyperlpiddemia in rat fed retinoic acid. Lipid. 16(7):496-501, 1981 https://doi.org/10.1007/BF02535047
  6. Raul, G.M., Ivete, A.R., Mario, H.H. Effects of triton WR-1339 and heparin on the transfer of surface lipids from triglycerideirich emulsions to HDL in rats. Lipid. 25(11):701-705, 1990 https://doi.org/10.1007/BF02544037
  7. Karen, F.K., Dawn, R.M., Linda, L.A., Steven, E.R., Leslie, D.S., James, M.W. In vivo Correction of LDL receptor deficiency in the WHHR with recombinant adenoviruses. J. Bio. Chem. 269(18):13695-13702, 1994
  8. Noriaki, K., Toru, K., Atsushi, M., Masayuki, Y., Kenji, I., Yutaka, N,, Chuichi, K. Induction of mRNA for LDL receptors in heterozygous Watanabe Heritable Hyperlipidemic Rabbit treated with CS-514(Pravastatin) and Cholestyramine. Circulation. 79(5):1084-1090, 1989 https://doi.org/10.1161/01.CIR.79.5.1084
  9. Mushkin, M.L., Dolgov, A.V. Activity of cholesterol metabolism enzymes and lipid levels in the rat liver, aorta, adrenals and serun after exposure to triton WR-1339. Vopr. Med. Khim. 32(3):98-101, 1986
  10. Robbins, S.L., Cotran, R., Kumar, V. Robbins pathologic basis of disease. W. B. Saunders. 4th edition. pp 556-561, 1989
  11. 방혜정, 강윤호. 고지혈증의 治方에 관한 문헌적 고찰. 동서의학 21(2):70-81, 1996
  12. Hayshi, H., Shitara, M., Yamasaki, F. The origin of lipid accumulated in the liver lysosomes after admimistration of triton WR-1339. J. Biochem. 92(5):1585-1590, 1982 https://doi.org/10.1093/oxfordjournals.jbchem.a134083
  13. Lottenberg, A.M., Oliveira, H.C., NAkandakare, E.R., Quintao, E.C. Effect of dietary fish oil on the rate of very LDL, triacyglycerol formation and on the metabolism of chylomicrons. Lipid. 27(5):326-330, 1992 https://doi.org/10.1007/BF02536145
  14. Zeniya, M., Reuben, A. Triton WR-1339-induced changes in serum lipids and biliary lipid secretion. Am. J. Physiol. 254(3):346-354, 1988
  15. Hornick, C.A., Kita, T., Hamilton, R.L., kane, J,P., Havel, R.J. Secretion of lipoproteins from the liver of normal and WHHR. Proc. Natl. Acad. Sci. USA. 80(19):6096-6100, 1983
  16. Sharma, R.D. Effect of various isoflavones on lipid levels in triton-treated rats. Atherosclerosis. 33: 371-375, 1979 https://doi.org/10.1016/0021-9150(79)90189-8
  17. Hayashi, H., Ninobi, S., Matsumoto, Y., Suga, T. Effect of triton WR-1339 on lipoprotein lipolytic activity and lipid content of rat liver lysosome. J. Biochem(Tokyo). 89(2):573-579, 1981
  18. Larusso, N.F., Kost, L.J., Carter, J.A., Barham, S.S. Triton WR-1339, a lysosomotropic compound, is excreted into bile and alters the biliary excretion of lysosomal enzymes and lipids. Hepatology. 2(2):209-215, 1982 https://doi.org/10.1002/hep.1840020204
  19. 김진택, 안상현, 박인식. Triton WR-1339주사에 의한 고지혈증 유발시 간세포내 지방축적에 관한 형태학적 연구. 동국대학교 한의학연구소 논문집 6: 107-115, 1997
  20. 김진택, 박인식, 안상현. Triton WR-1339주사에 의한 고지혈증 유발 생쥐 신장에 미치는 참홑파래 추출물의 지방축적 억제영향. 동국대학교 한의학연구소 논문집 6(2):87-98, 1998
  21. Baran, J.S., Laos, I., Langford, D.D., Miller, J.E., Jett, C., Taite, B., Tohrbacher, E. 3-Alkyl-3-hydroxyglutaric acid ; a new class of hypocholesterolemic HMG CoA reductase inhibitors. J. Med. Chem. 28(5):597-601, 1985 https://doi.org/10.1021/jm50001a011
  22. Goldfarb, S. Rapid increase in hepatic HMG CoA reductase activity and in vivo cholesterol synthesis after triton WR-1339 injection. J. Lipid. Res. 19(4):489-494, 1978
  23. Kasim, S.E., Leboeuf, R.C., Khilnani, S., Tallapaka, L., Dayananda, D., Jen, K.L. Mechanisms of triglyceride-lowering effect of an HMG-CoA reductase inhibitor in a hypertriglyceridemic animal model, the Zucker obese rat. J. Lipid. Res. 33(1):1-7, 1992
  24. Goldfarb, S.J. Rapid increase in hepatic HMG CoA reductase activity and in vivo cholesterol synthesis after Triton WR 1339 injection. Lipid Res., 19: 489-494, 1978
  25. Schlotz, M.C., Scanu, A., Page, I.M. Effect of triton on lipoprotein lipase of rat plasma. Am. J. Physiol. 188(2):399-402, 1957
  26. 이귀녕 외. 임상병리파일. 서울, 의학문화사, p 279, 1398, 1996
  27. 이규범. 임상병리핸드북. 서울, 고문사, pp 116-119, 1997
  28. 대한병리학회. 병리학. 서울, 고문사, pp 406-410, 2000
  29. 김명순 외. 생화학. 서울, 형설출판사, pp 142-144, 1999
  30. 김약수 외. 병리검사 매뉴얼. 서울, 고문사, p 14, 16, 26, 63, 1993
  31. 허준. 동의보감. 서울, 남산당, p 719, 1993
  32. 이시진. 본초강목. 서울, 고문사, p 980, 1975
  33. Yamada, S. Mycelial culture method of Phellinus linteus. Korean patent, pp 92-1194, 1994
  34. Choi, J.H., Ha, T.M., Kim, Y.H., Rho, Y.D. Studies on the main factors affecting the mycelial growth of Phellinus linteus. Kor. J. Mycol., 24: 214-222, 1996
  35. Maron, D.M., Ames, B.N. Revies methods for the Salmonella mutagenicity test. Mutation Res. 113: 173-215, 1983 https://doi.org/10.1016/0165-1161(83)90010-9
  36. Ji, J.H., Kim, M.N., Chung, C.K., Ham, S.S. Antimutagenic and cytotoxicity effects of Phellinus linteus extracts. J. Kor. Soc. Food Sci. Nutr., 29(2):322-328, 2000
  37. Ji, J.H., Kim, M.N., Chung, C.K., Ham, S.S. Antigenotoxic effects of Phellinus linteus and Agaricus blazei Murill extracts. J. Kor. Soc. Food Sci. Nutr., 29(3):513-517, 2000
  38. Jung, M.E., Ham, S.S., Nam, S.M., Kang, I.J., Kim, S.J., Chung, C.K. Biochemical and histological effects of Phellinus linteus methanol extract on liver lipid methabolism of rat fed CCl4 and high fat. J. Kor. Soc. Food Sci. Nutr. 30(2):331-337, 2001
  39. Lee, H.J., Lee, H.J., Park, J.M., Song, G.Y., Kang, K.S., Kim, S.H. Study on antitumor and immunomodulatory effects of Combodian Phellinus linteus. Kor. J. Oriental Physiology and Pathology 16(2):332-337, 2002
  40. Kim, J.S., Kim, J.H., Lee, H.J., Khil, J.H., Kim, S.H., Kim, D.H. A study on cytokine modulating effect of three origins of Phellinus linteus. Kor. J. Oriental Physiology and Pathology 17(4):898-904, 2003