Effect of Fermented Herbal Extracts, HP-1 on Enzyme Activities and Gene Expressions Related to Alcohol Metabolism in Ethanol-loaded Rats

발효한약추출물 HP-1이 알코올을 투여한 쥐의 알코올 대사에 미치는 영향

  • Jung, Yong-Joon (School of Biological Sciences, Seoul National University) ;
  • Han, Dong-Oh (Acupuncture & Meridian Sceince Research Center) ;
  • Choi, Bo-Hee (PaschaBio Co.,DY Co.) ;
  • Park, Chul (Acupuncture & Meridian Sceince Research Center) ;
  • Lee, Hye-Jung (Acupuncture & Meridian Sceince Research Center, Department of Acupuncture & Meridianology) ;
  • Kim, Sung-Hoon (Department of Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Hahm, Dae-Hyun (Acupuncture & Meridian Sceince Research Center, Institute of Oriental Medicine, Kyung Hee University)
  • 정용준 (서울대학교 자연과학대학 생명과학부) ;
  • 한동오 (경희대학교 침구경락과학연구센터) ;
  • 최보희 ((주)파스카바이오,(주)디와이) ;
  • 박철 (경희대학교 침구경락과학연구센터) ;
  • 이혜정 (경희대학교 침구경락과학연구센터, 경희대학교 한의과대학 경혈학교실) ;
  • 김성훈 (경희대학교 한의과대학 병리학교실) ;
  • 함대현 (경희대학교 침구경락과학연구센터, 경희대학교 한의학연구소)
  • Published : 2007.04.25

Abstract

Recently, much attention has been paid to developing various kinds of fermented herbal extracts, a new type of traditional herbal medicine, in the field of Korean traditional medicine. The fermentation of medicinal herbs is intended to exert a favorable influence on digestability, bioavailavility and pharmacological activity of herbal extract in the gastrointestinal tract. It also produces a number of fermentation products that intensify the nutritional and pharmacological aspects of the medicinal herbs. In order to develop a functional beverage of alleviating the aftereffects of the previous drinks, the extracts (HP-1) of fermented herbal mixture, including Artemisia capillaris Thunb., Lonicera japonica Thunberg, and Hovenia dulcis Thunb., were prepared and the medicinal effect as a hangover cure was evaluated in ethanol-loaded rats. The enzyme activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase-2 (ALDH2) were analyzed by measuring the concentration of blood ethanol and acetaldehyde. The mRNA expression of ADH and ALDH2 was also investigated through RT-PCR analysis. In the HP-1-treated group, the concentration of blood ethanol was significantly reduced at one hour after loading of ethanol, as compared to that in the saline-treated group. The reduced ethanol was converted to acetaldehyde, which resulted in rapid increase in acetaldehyde concentration in an hour. Acetaldehyde was started to decrease at 5 hours after ethanol loading. It implies that HP-1 is highly effective to stimulate the activities of ADH and ALDH2. The HP-1 treatment also activated the mRNA expression of ADH and ALDH. This study suggests that fermented herbal extract, HP-1 can be used as a functional beverage of alleviating the alcohol-induced hangover symptoms by stimulating the activities and gene expression of hepatic alcohol metabolizing enzymes.

Keywords

References

  1. Swift, R. and Davidson, D. Alcohol hangover, mechanisms and mediators. Alcohol Health Res. World. 22: 54-60, 1998
  2. Lieber, C.S. Alcohol and the liver: Metabolism of ethanol, metabolic effects and pathogenesis of injury. Acta. Med. Scand. (Suppl). 703: 11-55, 1985
  3. Theorell, H.S. and Ronnichsen, R. Studies on liver alcohol dehydrogenase. I. Equilibria and initial reaction velocities. Acta. Chem. Scand. 5: 1105-1126, 1951 https://doi.org/10.3891/acta.chem.scand.05-1105
  4. Marjanen, L. Intracelluar localization of aldehyde dehydrogenase in rat liver. Biochem. J. 127: 663-639, 1972
  5. Gill, K., Amit, Z. and Smith, B.R. The regulation of alcohol consumption in rats: the role of alcohol, metabolizing enzymes, catalase and aldehyde dehydrogenase. Alcohol 13(4):347-355, 1996 https://doi.org/10.1016/0741-8329(96)00006-7
  6. Sur, J.S. Alcohol metabolism and nutritional effects. Food Indus. Nutr. 4: 13-19, 1999
  7. Lieber, C.S. and Decarli, L.M. Hepatic microsomal ethanol oxidizing system: In vitro characteristics and adaptive properties in vivo. J. Biol. Chem. 245: 2505-2512, 1970
  8. Lieber, C.S. Liver adaptation and injury in alcoholism. New Eng. J. Med. 288: 356-362, 1973 https://doi.org/10.1056/NEJM197302152880710
  9. Kim, C.I. Cause and effect of hangover. Food Indus. Nutr. 4: 26-30, 1999
  10. Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. Lipids. In: Harper's Biochemistry. Appleton & Lange, Connecticut, p 260, 1993
  11. Kim, K.W., Yang, J.S., Lee, J.S., Cho, Y. S., Kang, S. K. and Chung, H.K. Activity of alcohol dehydrogenase and ethanol, acetaldehyde levels in normal adults blood. Korean Indus. Hyg. Assoc. J. 4: 240-247, 1994
  12. Helander, A. and Tottmar, O. Effect of acute ethanol administration on human blood aldehyde dehydrogenase activity. Alcohol Clin. Exp. Res. 12: 643-646, 1988 https://doi.org/10.1111/j.1530-0277.1988.tb00257.x
  13. Nordmann, R., Ribiere, C. and Rouach, H. Implication of free radical mechanisms in ethanol-induced cellular injury. Free Radic. Biol. Med. 12: 219-240, 1992 https://doi.org/10.1016/0891-5849(92)90030-K
  14. Lieber, C.S. Ethanol metabolism, cirrhosis and alcoholism. Clin. Chem. Acta. 257(1):59-84, 1997 https://doi.org/10.1016/S0009-8981(96)06434-0
  15. 김창민, 신민규, 안덕균, 이경순 外. 完譯中藥大辭典. (주)鼎談, 2: 582-587, 7: 3500-3506, 8: 3938-3939, 1999