Protective Effect of Pueraria Radix Extract on the Cisplatin-induced Cytotoxicity of HEI-OC1 Cells Via Scavenging of Free Radicals

갈근 추출물이 Cisplatin으로 손상된 HEI-OC1 청각세포보호와 유리라디칼 소거능에 미치는 영향

  • Yu, Hyeon-Hee (Department of Food and Nutrition, Kunsan National University) ;
  • Seo, Se-Jeong (Department of Oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, VCRC) ;
  • Moon, Hae-Dalma (Department of Oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, VCRC) ;
  • Park, Rae-Kil (Vestibulocochlear Research Center & Department of Microbiology, School of Medicine, VCRC) ;
  • So, Hong-Seob (Vestibulocochlear Research Center & Department of Microbiology, School of Medicine, VCRC) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine) ;
  • Jung, Su-Young (Department of Food and Nutrition, Wonkwang University)
  • 유현희 (군산대학교 식품영양학과) ;
  • 서세정 (원광대학교 치과대학 구강생화학교실) ;
  • 문해닮아 (원광대학교 치과대학 구강생화학교실) ;
  • 박래길 (원광대학교 의과대학 미생물학과) ;
  • 소홍섭 (원광대학교 의과대학 미생물학과) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 정수영 (원광대학교 식품영양학과)
  • Published : 2007.04.25

Abstract

The radix of Pueraria thunbergiana BENTHAM (Leguminosae) is traditionally prescribed to attenuate the clinical manifestations of inner ear dysfunction and various clinical situations including fever, gastrointestinal disorders, skin problems, migraine headaches, lowering cholesterol and treating chronic alcoholism in Oriental Medicine. In the present study, we examined the effect of ethanol extract of P. thunbergiana radix (EPR) on cisplatin-mediated HEI-OC1 auditory cell death. In addition, to investingate the protection mechanism of EPR on free radicals. Treatment of EPR protected cells from cisplatin and reduced lipid peroxidation in a dose-dependent manner. Furthermore, EPR demonstrated significant scavenging activity against various free radicals, including superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. These results indicate that EPR protects cisplatin-induced damages of HEI-OC1 cells through inhibition of lipid peroxidation and augmenting scavenging activities against free radials.

Keywords

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