독활로부터 항치아우식 활성을 가진 stigmasterol 분리

Isolation of Anti-cariogenic Agent, Stigmasterol, from Aralia continentali

  • 유현희 (군산대학교 식품영양학과) ;
  • 문해닮아 (원광대학교 치과대학 구강생화학교실) ;
  • 황지영 (원광대학교 치과대학 구강생화학교실) ;
  • 김선영 (원광대학교 치과대학 구강생화학교실) ;
  • 정승일 (전주생물소재연구소) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 유용욱 (원광대학교 치과대학 구강생화학교실)
  • Yu, Hyeon-Hee (Department of Food and Nutrition, Kunsan National University) ;
  • Moon, Hae-Dalma (Department of Oral Biochemistry and VCRC, School of Dentistry, Wonkwang University) ;
  • Hwang, Ji-Young (Department of Oral Biochemistry and VCRC, School of Dentistry, Wonkwang University) ;
  • Kim, Seon-Young (Department of Oral Biochemistry and VCRC, School of Dentistry, Wonkwang University) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • You, Yong-Ouk (Department of Oral Biochemistry and VCRC, School of Dentistry, Wonkwang University)
  • 발행 : 2007.02.25

초록

In the present study, we has been isolated the anti-cariogenic component, stigmasterol, from Aralia continentalis (A. continentalis) and identified by MS, $^1$H-NMR and $^{13}$C-NMR and also investigated the anti-cariogenic properties of stigmasterol. The methanol extract of ,A. continentalis showed concentration-dependent inhibitory activity against the growth and acid production of S. mutans. The MeOH extract was suspended in H$_2$O and sequentially partitioned with n-hexane, CHCl$_3$, EtOAc, and n-BuOH. The CHCl$_3$ fraction showed remarkable antibacterial activity against S. mutans. The anti-cariogenic compound, stigmasterol, has been isolated successively through the screening system and various chromatography methods. Anti-cariogenic properties of stigmasterol were also investigated. From this active chloroform subfraction, isolation and identification finally gave (24E)-stigmasta-5,22-dien-3${\beta}$-ol (stigmasterol) {[a]$_D\;^{25}$ -48.33$^{\circ}C$(C 0.28, CHCl$_3$)} by spectroscopic methods (MS, $^1$H-NMR and $^{13}$C-NMR) as an active principle. The compound, stigmasterol, showed significant growth, acid production, adhesion and water-insoluble glucan synthesis inhibitory effect against S. mutans. These results suggest that stigmasterol from ,A. continentalis may inhibit cariogenic properties of S. mutans and these properties may provide some scientific rationales that the local inhabitants used the extracts for treatment of dental diseases.

키워드

참고문헌

  1. 한국구강보건협회. 한국인 구강질환 실태조사 결과보고, 1976
  2. 김무길. 대도시인의 구강보건실태 및 상대구강보건의료수요 조사연구. 대한구강보건학회지, 4(1):19-44, 1980
  3. 박종만. 소도시인의 구강보건실태 및 상대구강보건의료수요 조사연구. 대한구강보건학회지, 5(1):7-33, 1981
  4. 오상일, 김종배. 비도시인의 구강보건실태 및 상대구강보건의료수요 조사연구. 대한구강보건학회지, 5(1):55-82, 1981
  5. 김종배 외. 국민구강건강조사 보고서, 1991
  6. 국민구강보건연구소. 1995년 국민구강건강조사보고서, 1995
  7. 보건복지부. 2003년 국민구강건강실태조사보고서. 2004
  8. http://www.who.int/세계보건기구, 2001
  9. Tsuneno, N., Masa, T., Masao, H. Dental caries prevention by traditional chinese medicines. Plant Med 44:100-106, 1982 https://doi.org/10.1055/s-2007-971412
  10. 김종배, 최유진. 공중구강보건학. 서울, 고문사, pp 68-84, 1990
  11. 허준 편. 원저 동의학 연구소 역. 동의보감. 서울, 여강출판사, p 773, 1994
  12. 허준 편. 동의보감 국역 위원회 역. 동의보감. 서울, 법인문화사, p 1632, 1999
  13. 유현희, 서세정, 김연화, 이혜연, 금기천, 나종찬, 전병훈, 유용욱. 독활 메탄올 추출물의 Streptococcus mutans에 대한 성장, 산생성, 부착 및 비수용성 글루칸 합성 억제에 미치는 영향. 동의생리병리학회지, 19(1):87-91, 2003
  14. 유현희, 정승일, 정수영, 문해닮아, 황지영, 정한솔, 유용욱 독활로부터 분리한 continentalic acid의 항치아우식 활성. 동의생리병리학회지, 20(3):617-622, 2006
  15. Kang, S.S., Park, J.G., Hyun, J.W., Sung, M.S., Yang, Y.M., Chang, H.S., Paik, W.H. The cytotoxic activity of sterol derivatives from Pulsatilla Chinensis Regal, J Kor Cancer 28(1):146-150, 1996
  16. Bae, K.H., Seo, W.J., Kwon, T.H., Baek, S.H., Lee, S.W., Jin, K.D. Anticariogenic ${\beta}$-carboline alkaloids from Commelina communis. Arch Pharm Res 15(3):220-223, 1992 https://doi.org/10.1007/BF02974058
  17. Koo, H., Gomes, B.P., Rosalen, P.L., Ambrosano, G.M., Park, Y.K., Cury, J.A. In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Arch Oral Biol 45(2):141-148. 2000 https://doi.org/10.1016/S0003-9969(99)00117-X