Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Influence of Extracts of Biotae Orientalis on the Efficiency of Hair Growth in Hair Removed C57BL/6N Mice

측백엽(側柏葉) 추출물의 발모효과(發毛效果)에 관한 실험적 연구

  • Tak, Min-Jin (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Jung, Il-Kook (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Kim, Dae-Keun (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Jung, Han-Sol (Department of Oriental Pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
  • 탁민진 (우석대학교 한의과대학 해부학교실) ;
  • 정일국 (우석대학교 한의과대학 해부학교실) ;
  • 김대근 (우석대학교 한의과대학 해부학교실) ;
  • 정한솔 (우석대학교 한의과대학 한방병리학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Published : 2007.02.25
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Abstract

Biotae Orientalis has been widely used for treatment of relaxion of smooth muscle, gastrointestinal hemorrhage and alopecia in Oriental Medicine. This experiment examined the effect of an extracts, obtained from the acetone and MeOH extracts of dried or fresh Biotae Orientalis, on hair growing activity of the C57BL/6N mice after topical application to skin. First, We examined on hair growth activity of extracts of Biotae Orientalis compare to control and 1% minoxidil groups. Second, We investigated on the number of hair follicle and mast cells after topical application of extracts of the Biotae Orientalis to skin for 16 day. Third, We investigated immunoreactive density of vascular endothelial growth factor(VEGF), protein kinase C-${\alpha}$(PKC-${\alpha}$) and stem(mast) cell factor(SCF) in skin of C57BL/6N mice by immunohistochemical methods. I fourth investigated changes of subpopulation of splenocytes and thymocytes in C57BL/6N mice for 16day using laser flow cytometry. The results were as follows : Hair growing effect of acetone and MeOH extracts of dried and fresh Biotae Orientalis was observed in 70%, 90% and 60% in hair removed skin area in 16 day respectively. Immunoreactive density of VEGF and PKC-${\alpha}$ in skin of experimental groups was weakly stained compare to control group in 10 day. Immunoreactive density of stem cell factor in skin of experimental group was heavily stained compare to control group in 10 day. Splenic TH/TC Iymphocytes of lived MeOH extracts group significantly increased compare to control group. TH cells in thymic T lymphocytes were increased compare to control group. These experiment suggest that acetone and MeOH extracts of Biotae Orientalis may be used for topical treatment of alopecia areata.

Keywords

References

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