Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Development of Biologically Active Compounds from Edible Plant Sources-XX. Isolation of Lipids from the Roots of Brassica campestris ssp rapa

식용식물자원으로부터 활성물질의 탐색-XX. 순무(Brassica campestris ssp rapa)뿌리로부터 지질화합물의 분리

  • Bang, Myun-Ho (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Dae-Young (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Han, Min-Woo (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Oh, Young-Jun (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Chung, Hae-Gon (Ganghwa Agricultural R&D Center) ;
  • Jeong, Tae-Sook (National Research Laboratory of Lipid Metabolism & Atherosclerosis, KRIBB) ;
  • Choi, Myung-Sook (Department of Biochemistry, College of Pharmacy, Kyung Hee University) ;
  • Lee, Kyung-Tae (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • 방면호 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이대영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 한민우 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 오영준 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 정해곤 (강화군 특화작목연구소) ;
  • 정태숙 (한국생명공학연구원 지질대사연구실) ;
  • 최명숙 (경희대학교 약학대학 약품생화학교실) ;
  • 이경태 (경북대학교 식품영양학과 영양생화학실) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2007.09.30
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Abstract

The roots of Brassica campestris ssp rapa were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_{2}O$. From the EtOAc and n-BuOH fractions, five compounds were isolated through the repeated silica gel column chromatographies. From the result of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as palmitic acid methyl ester (1), linolenic acid methyl ester (2), linoleic acid methyl ester (3), ${\beta}-sitosterol$ (4) and daucosterol (5).

순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH 추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, $H_{2}O$분획으로 나누었으며, EtOAc분획과 n-BuOH분획에 대해 silica gel 및 ODS column chromatography를 실시하여 5종의 화합물을 분리 정제하였다. $^{1}H-NMR$, $^{13}C-NMR$, DEPT spectrum 및 Mass spectrum 등을 통하여 palmitic acid methyl ester(1), linolenic acid methyl ester(2), linoleic acid methyl ester(3), ${\beta}-sitosterol$(4), daucosterol(5)으로 구조를 결정하였다.

Keywords

References

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