Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Antiproliferative Effect of RST Associated with the Inhibition of Cyclooxygenase-2 Expression and Prostaglandin E2 Release in Human Lung Carcinoma Cells

산두근 추출물이 인체폐암세포의 COX-2 발현 및 PGE2 생성에 미치는 영향

  • Kim, Kang-Tae (Department of Pathology, College of Oriental Medicine, Dongeui University) ;
  • Eom, Hyun-Sup (Department of Pathology, College of Oriental Medicine, Dongeui University) ;
  • Chi, Gyoo-Yong (Department of Pathology, College of Oriental Medicine, Dongeui University)
  • 김강태 (동의대학교 한의과대학 병리학교실) ;
  • 엄현섭 (동의대학교 한의과대학 병리학교실) ;
  • 지규용 (동의대학교 한의과대학 병리학교실)
  • Published : 2007.08.25
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Abstract

In this study the effect of water extract of Sophora tonkinensis Gapnep (RST) was investigated on the growth of human lung carcinoma A549 cells. Exposure of A549 cells to RST resulted in the growth inhibition in a dose-dependent manner as measured by MTT assay. The antiproliferative effect by RST treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. RST treatment did not induce the cell cycle arrest and the levels of tumor suppressor p53 as well as cyclin-dependent kinase inhibitor p21(WAF1/CIP1). It was found that RST treatment decreased the levels of cyclooxygenase (COX) -2 mRNA and protein expression without significant changes in the expression of COX-1 and inducible nitric oxide synthase (iNOS), which was correlated with a decrease in prostaglandin E2 (PGE2) synthesis. RST treatment also slightly inhibited the levels of human telomerase reverse transcriptase (hTERT) mRNA and protein expression, and the activity of telomerase. Taken together, these findings suggested that RST-induced inhibition of human lung carcinoma A549 cell growth was aoosciated with the inhibition of COX-2 expression and PGE2 production. These results provided important new insights into the possible molecular mechanisms of the anti-cancer activity of RST.

Keywords

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