Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of 2-Chloromethyl-1-Dihydroxyphosphinylpyrrolidine(2C-1DPP) on Differentiation Induction of Human Leukemia HL-60 Cells

2-Chloromethyl-1-Dihydroxyphosphinylpyrrolidine (2C-1DPP)에 의한 백혈병 세포주 HL-60의 분화유도 효과

  • Kim, Youg-Mi (Division of Natural Science, College of Natural Sciences, Wonkwang University) ;
  • Ju, Seong-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Jun-Ho (Division of Natural Science, College of Natural Sciences, Wonkwang University) ;
  • Oh, Jung-Mi (Division of Bio-Nano Chemistry, College of Natural Sciences, Wonkwang University) ;
  • Lee, Chae-Ho (Division of Bio-Nano Chemistry, College of Natural Sciences, Wonkwang University) ;
  • Kim, Eun-Cheol (Division of Oral and Maxillofacial Pathology, College of Dentistry, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Won-Sin (Division of Natural Science, College of Natural Sciences, Wonkwang University) ;
  • Kim, Won-Sin (Division of Natural Science, College of Natural Sciences, Wonkwang University)
  • 김영미 (원광대학교 자연과학대학 생명과학부) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 박준호 (원광대학교 자연과학대학 생명과학부) ;
  • 오정미 (원광대학교 자연과학대학 생명나노화학부) ;
  • 이채호 (원광대학교 자연과학대학 생명나노화학부) ;
  • 김은철 (원광대학교 치과대학 구강병리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 김원신 (원광대학교 자연과학대학 생명과학부) ;
  • 김원신 (원광대학교 자연과학대학 생명과학부)
  • Published : 2007.08.25
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Abstract

We have examined the induction of HL-60 cell differentiation by treatment of 2-chloromethyl-1-dihydroxyphosphinyl pyrrolidine(2C-1DPP), which is derivative of piperidine and pyrrolidine by ${\alpha}-phosphoramidoakylation$ reaction. It was observed that HL-60 cell proliferation was dose- and time-dependently inhibited by treatment with 2C-1DPP. 2C-1DPP treatment caused a significant change in NBT reduction and enhanced ATRA-induced NBT reduction. Treatment of 2C-1DPP to HL-60 cells increased only CD11b expression in the cells, and also increased markedly G0/G1 stage arrest of HL-60 cells. These results can suggest that 2C-1DPP induced the differentiation of HL-60 cells to granulocytes lineage and enhanced ATRA-induced differentiation. Moreover, DNA expression levels of p27 were up-regulated during 2C-1DPP-dependent HL-60 cell differentiation. Our results suggest that 2C-1DPP have potential as a therapeutic agent in human leukemia.

Keywords

References

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