Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Capsosiphon Fulvescens Decreases Melanin Synthesis Via Downregulation of Tyrosinase and TRP-2 Expression

  • Yoo, Hyun-Ju (The Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Jo, Mi-Gyeong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Park, Si-Jun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Pyo, Hyeong-Bae (R&D Center, Hanbul Cosmetics Co.) ;
  • Lim, Kyu-Sang (The Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ki-Nam (The Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • Published : 2007.08.25
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Abstract

The green marine algae, Capsosiphon fulvescens (CF) is a food supplement cultivated in south coast of Southern Korea. The purpose of this study was to investigate the mechanism of CF-induced hypopigmentation. The present study was designed to determine the effect of CF extracton melanogenesis in B16 cells, particularly its specific effects on tyrosinase and tyrosinase-related protein 2 (TRP-2). We measured melanin contents and analyzed melanosome associated protein levels using Western blot and Reverse transcription-polymerase chian reaction (RT-PCR) analysis. CF extract markedly inhibited melanin synthesis and tyrosinase activity. In addition, cellular dendricity was slightly decreased by CF extract. In further experiments, CF extract significantly reduced the protein levels of tyrosinase and TRP-2 in B16 cells. RT-PCR analysis revealed that tyrosinase and TRP-2 mRNA levels were unaffected by CF treatment. Therefore, these results suggest that hypopigmentary effect of CF was due to post-translational degradationof tyrosinase and TRP-2.

Keywords

References

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