Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Herbal Cocktail Sagunja Protects $H_2O_2$-induced H9c2 Cardiomyoblast Cell Death through the Induction of Heme Oxygenase-1

  • Park, Chan-Ny (Vestibulo Cochlear Research Center and Department of Microbiology, School of Medicine) ;
  • Moon, Byung-Soon (Department of Internal Medicine School of Oriental Medicine) ;
  • Jeon, Seon-Bok (Department of Public Health, Graduate School of Wonkwang University) ;
  • Kim, Nam-Song (Department of Public Health, Graduate School of Wonkwang University) ;
  • Chung, Sang-Young (Department of Surgery, Chonnam National University Medical School) ;
  • Park, Jin-Woo (Department of Biochemistry, Chonbuk National University) ;
  • Park, Rae-Kil (Vestibulo Cochlear Research Center and Department of Microbiology, School of Medicine)
  • Published : 2007.08.25
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Abstract

Sagunjatang (Sagunja), containing Radix Astragali, Radix Ginseng, Fructus Schizandrae, Radix Ophiopogonis and Radix Glycyrrhizae, has been used as a prescription for ischemic heart and brain diseases in Korean traditional medicine. This study was designed to investigate the protective mechanisms of Sagunja on $H_2O_2$-induced cytotoxicity of H9c2 cardiomyocytes. Treatment with $H_2O_2$ resulted in death of H9c2 cells, characterized by apparent apoptotic features, including the fragmentation of nucleus and increase in sub-GO/G1fraction of cell cycle. However, Sagunja markedly suppressed the apoptotic characteristics of H9c2 cells induced by $H_2O_2$ with decrease of intracellular peroxide level. In addition, Sagunja suppressed the features of mitochondrial dysfunction, including change of mitochondrial membrane potential, in $H_2O_2$- treated cells. Additionally, Sagunja induced the expression of HO-1 protein in both time-and dose-dependent manner. The role of HO-1 in ROS-scavenging activity of Sagunja is proposed.

Keywords

References

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