The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Single-Channel Recording of TASK-3-like $K^+$ Channel and Up-Regulation of TASK-3 mRNA Expression after Spinal Cord Injury in Rat Dorsal Root Ganglion Neurons

  • Jang, In-Seok (Department of Thoracic Surgery, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • La, Jun-Ho (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim, Gyu-Tae (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Lee, Jeong-Soon (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim, Eun-Jin (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Lee, Eun-Shin (Department of Rehabilitation Medicine, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim, Su-Jeong (Yeungnam University Institute of Biomedical Engineering, Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Seo, Jeong-Min (Yeungnam University Institute of Biomedical Engineering, Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Ahn, Sang-Ho (Yeungnam University Institute of Biomedical Engineering, Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Park, Jae-Yong (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Hong, Seong-Geun (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kang, Da-Won (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Han, Jae-Hee (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University)
  • Published : 2008.10.31
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Abstract

Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain $K^+$ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like $K^+$ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like $K^+$ channel was $33.0{\pm}0.1$ pS at - 60 mV, and TASK-3 activity fell by $65{\pm}5%$ when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG $K^+$ channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background $K^+$ channel under SCI-induced acidic condition.

Keywords

References

  1. Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma 12: 1−21, 1995 https://doi.org/10.1089/neu.1995.12.1
  2. Braughler JM, Hall ED. Effects of multi-dose methylprednisolone sodium succinate administration on injured cat spinal cord neurofilament degradation and energy metabolism. J Neurosurg 61: 290−295, 1984
  3. Cooper BY, Johnson RD, Rau KK. Characterization and function of TWIK-related acid sensing $K^+$ channels in a rat nociceptive cell. Neuroscience 129: 209−224, 2004
  4. Farooque M, Hillered L, Holtz A, Olsson Y. Effects of methylprednisolone on extracellular lactic acidosis and amino acids after severe compression injury of rat spinal cord. J Neurochem 66: 1125−1130, 1996
  5. Holzer P. Acid-sensitive ion channels in gastrointestinal function. Curr Opin Pharmacol 3: 618−625, 2003
  6. Kang D, Choe C, Kim D. Thermosensitivity of the two-pore domain $K^+$ channels TREK-2 and TRAAK. J Physiol 564: 103−116, 2005
  7. Kang D, Han J, Talley EM, Bayliss DA, Kim D. Functional expression of TASK-1/TASK-3 heteromers in cerebellar granule cells. J Physiol 554: 64−77, 2004a
  8. Kang D, Kim D. Single-channel properties and pH sensitivity of two-pore domain K+ channels of the TALK family. Biochem Biophys Res Commun 315: 836−844, 2004
  9. Kang D, Kim D. TREK-2 (K2P10.1) and TRESK (K2P18.1) are major background K+ channels in dorsal root ganglion neurons. Am J Physiol Cell Physiol 291: C138−146, 2006
  10. Kang D, Mariash E, Kim D. Functional expression of TRESK-2, a new member of the tandem-pore $K^+$ channel family. J Biol Chem 279: 28063−28070, 2004b
  11. Kim D. Fatty acid-sensitive two-pore domain K+ channels. Trends Pharmacol Sci 24: 648−654, 2003
  12. La JH, Kang D, Park JY, Hong SG, Han J. A novel acid-sensitive K (＋) channel in rat dorsal root ganglia neurons. Neurosci Lett 406: 244−249, 2006
  13. Lang-Lazdunski L, Bachet J, Rogers C. Repair of the descending thoracic aorta: impact of open distal anastomosis technique on spinal cord perfusion, neurological outcome and spinal cord histopathology. Eur J Cardiothorac Surg 26: 351−358, 2004
  14. Medhurst AD, Rennie G, Chapman CG, Meadows H, Duckworth MD, Kelsell RE, Gloger, II, Pangalos MN. Distribution analysis of human two pore domain potassium channels in tissues of the central nervous system and periphery. Brain Res Mol Brain Res 86: 101−114, 2001
  15. Oh U, Hwang SW, Kim D. Capsaicin activates a nonselective cation channel in cultured neonatal rat dorsal root ganglion neurons. J Neurosci 16: 1659−1667, 1996
  16. Rau KK, Cooper BY, Johnson RD. Expression of TWIK-related acid sensitive $K^+$ channels in capsaicin sensitive and insensitive cells of rat dorsal root ganglia. Neuroscience 141: 955−963, 2006
  17. Rush AM, Cummins TR, Waxman SG. Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons. J Physiol 579: 1−14, 2007
  18. Smith GT. Pharmacological characterization of ionic currents that regulate high-frequency spontaneous activity of electromotor neurons in the weakly electric fish, Apteronotus leptorhynchus. J Neurobiol 66: 1−18, 2006
  19. Zhang XF, Gopalakrishnan M, Shieh CC. Modulation of action potential firing by iberiotoxin and NS1619 in rat dorsal root ganglion neurons. Neuroscience 122: 1003−1011, 2003