The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Forskolin Enhances Synaptic Transmission in Rat Dorsal Striatum through NMDA Receptors and PKA in Different Phases

  • Cho, Hyeong-Seok (Department of Pharmacology, College of Medicine, The Catholic University of Korea) ;
  • Lee, Hyun-Ho (Department of Pharmacology, College of Medicine, The Catholic University of Korea) ;
  • Choi, Se-Joon (Department of Pharmacology, College of Medicine, The Catholic University of Korea) ;
  • Kim, Ki-Jung (Department of Pharmacology, College of Medicine, The Catholic University of Korea) ;
  • Jeun, Seung-Hyun (Department of Pharmacology, College of Medicine, The Catholic University of Korea) ;
  • Li, Qing-Zhong (Department of Pharmacology, College of Medicine, The Catholic University of Korea) ;
  • Sung, Ki-Wug (Department of Pharmacology, College of Medicine, The Catholic University of Korea)
  • Published : 2008.12.31
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Abstract

The effect of forskolin on corticostriatal synaptic transmission was examined by recording excitatory postsynaptic currents (EPSCs) in rat brain slices using the whole-cell voltage-clamp technique. Forskolin produced a dose-dependent increase of corticostriatal EPSCs (1, 3, 10, and $30{\mu}M$) immediately after its treatment, and the increase at 10 and $30{\mu}M$ was maintained even after its washout. When the brain slices were pre-treated with (DL)-2-amino-phosphonovaleric acid (AP-V, $100{\mu}M$), an NMDA receptor antagonist, the acute effect of forskolin ($10{\mu}M$) was blocked. However, after washout of forskolin, an increase of corticostriatal EPSCs was still observed even in the presence of AP-V. When KT 5720 ($5{\mu}M$), a protein kinase A (PKA) inhibitor, was applied through the patch pipette, forskolin ($10{\mu}M$) increased corticostriatal EPSCs, but this increase was not maintained. When forskolin was applied together with AP-V and KT 5720, both the increase and maintenance of the corticostriatal EPSCs were blocked. These results suggest that forskolin activates both NMDA receptors and PKA, however, in a different manner.

Keywords

References

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