The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Voltage-dependent $Ca^{2+}$ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach

  • Kim, Young-Chul (Department of Physiology, Chungbuk National University, College of Medicine) ;
  • Suzuki, Hikaru (Department of Physiology, Nagoya City University Medical School) ;
  • Xu, Wen-Xie (Department of Physiology, College of Medicine, Shanghai Jiaotong University) ;
  • Hashitani, Hikaru (Department of Physiology, Nagoya City University Medical School) ;
  • Choi, Woong (Department of Pharmacology, Chungbuk National University, College of Medicine) ;
  • Yun, Hyo-Yung (Department of Surgery, Chungbuk National University, College of Medicine) ;
  • Park, Seon-Mee (Department of Internal Medicine, Chungbuk National University, College of Medicine) ;
  • Youn, Sei-Jin (Department of Internal Medicine, Chungbuk National University, College of Medicine) ;
  • Lee, Sang-Jeon (Department of Surgery, Chungbuk National University, College of Medicine) ;
  • Lee, Sang-Jin (Department of Physiology, Chungbuk National University, College of Medicine)
  • Published : 2008.12.31
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Abstract

The properties of voltage dependent $Ca^{2+}$ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times $min^{-1}$ respectively, in the presence of $1{\mu}M$ nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under $K^+$ -rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With $Cs^+$-rich pipette solution at $V_h=-80\;mV$, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine ($1{\mu}M$) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent $Ca^{2+}$ currents ($VDCC_L$).

Keywords

References

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