The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patients

  • Park, Kyoung-Sun (Division of Molecular and Life Sciences, Hanyang University) ;
  • Choi, Mi-Ran (Division of Molecular and Life Sciences, Hanyang University) ;
  • Jung, Kyoung-Hwa (Division of Molecular and Life Sciences, Hanyang University) ;
  • Kim, Seung-Hyun (Department of Neurology, Hanyang University Hospital) ;
  • Kim, Hyun-Young (Department of Neurology, Hanyang University Hospital) ;
  • Kim, Kyung-Suk (Bioengineering Institute, CoreStem Inc.) ;
  • Cha, Eun-Jong (Department of Biomedical Engineering, College of Medicine, Chungbuk National University) ;
  • Kim, Yang-Mi (Department of Physiology, College of Medicine, Chungbuk National University) ;
  • Chai, Young-Gyu (Division of Molecular and Life Sciences, Hanyang University)
  • Published : 2008.12.31
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Abstract

Human bone marrow mesenchymal stem cells (hBM-MSCs) represent a potentially valuable cell type for clinical therapeutic applications. The present study was designed to evaluate the effect of long-term culturing (up to $10^{th}$ passages) of hBM-MSCs from eight individual amyotrophic lateral sclerosis (ALS) patients, focusing on functional ion channels. All hBM-MSCs contain several MSCs markers with no significant differences, whereas the distribution of functional ion channels was shown to be different between cells. Four types of $K^+$ currents, including noise-like $Ca^{+2}$-activated $K^+$ current ($IK_{Ca}$), a transient outward $K^+$ current ($I_{to}$), a delayed rectifier $K^+$ current ($IK_{DR}$), and an inward-rectifier $K^+$ current ($K_{ir}$) were heterogeneously present in these cells, and a TTX-sensitive $Na^+$ current ($I_{Na,TTX}$) was also recorded. In the RT-PCR analysis, Kv1.1,, heag1, Kv4.2, Kir2.1, MaxiK, and hNE-Na were detected. In particular, ($I_{Na,TTX}$) showed a significant passage-dependent increase. This is the first report showing that functional ion channel profiling depend on the cellular passage of hBM-MSCs.

Keywords

References

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