Anti-metastatic Effect of Samguikoeui-Tang Via Inhibition of Matrix Metalloproteinases Activities

금속단백분해효소의 활성 저해를 통한 삼귀고의탕의 전이억제 효과

  • Kim, Sung-Moo (Cancer Preventive Material Development Research Center, Kyunghee University) ;
  • Rhee, Yun-Hee (Cancer Preventive Material Development Research Center, Kyunghee University) ;
  • Lee, Joo-Ho (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim, Sung-Hoon (Cancer Preventive Material Development Research Center, Kyunghee University) ;
  • Lee, Eun-Ok (Cancer Preventive Material Development Research Center, Kyunghee University)
  • 김성무 (경희대학교 한의과대학 암예방소재개발연구센터) ;
  • 이연희 (경희대학교 한의과대학 암예방소재개발연구센터) ;
  • 이주호 (경희대학교 동서의학대학원) ;
  • 김성훈 (경희대학교 한의과대학 암예방소재개발연구센터) ;
  • 이은옥 (경희대학교 한의과대학 암예방소재개발연구센터)
  • Published : 2008.12.25

Abstract

This study was performed to examine the anti-metastatic effect of ethanol extract of Samguikoeui-Tang (SGKE), a formula consisting of four oriental herbs, in highly-metastatic HT1080 human fibrosarcoma cells. SGKE significantly inhibited the adhesion of HT1080 cells to matrigel at nontoxic concentrations in a dose-dependent manner, while it did not exert cytotoxicity against HT1080 cells up to the concentration of 100 ${\mu}g$/ml. Also, SGKE depressed the activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) by gelatin zymography. However, SGKE did not affect the mRNA expression of MMP-2 and TIMP-2, an inhibitor of MMP-2, by RT-PCR analysis. In addition, the effect of SGKE on HT1080 cell invasion was determined using Boyden chamber assay. SGKE suppressed the invasion of HT1080 cells in a dose-dependent manner. Taken together, these results suggest that SGKE has an anti-metastatic effect via inhibition of MMP-2 and -9 activities.

Keywords

References

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